19 research outputs found
The NIH-NIAID Filariasis Research Reagent Resource Center
Filarial worms cause a variety of tropical diseases in humans; however, they are difficult to study because they have complex life cycles that require arthropod intermediate hosts and mammalian definitive hosts. Research efforts in industrialized countries are further complicated by the fact that some filarial nematodes that cause disease in humans are restricted in host specificity to humans alone. This potentially makes the commitment to research difficult, expensive, and restrictive. Over 40 years ago, the United States National Institutes of Health–National Institute of Allergy and Infectious Diseases (NIH-NIAID) established a resource from which investigators could obtain various filarial parasite species and life cycle stages without having to expend the effort and funds necessary to maintain the entire life cycles in their own laboratories. This centralized resource (The Filariasis Research Reagent Resource Center, or FR3) translated into cost savings to both NIH-NIAID and to principal investigators by freeing up personnel costs on grants and allowing investigators to divert more funds to targeted research goals. Many investigators, especially those new to the field of tropical medicine, are unaware of the scope of materials and support provided by the FR3. This review is intended to provide a short history of the contract, brief descriptions of the fiilarial species and molecular resources provided, and an estimate of the impact the resource has had on the research community, and describes some new additions and potential benefits the resource center might have for the ever-changing research interests of investigators
RecombinantWolbachia surface protein (WSP)-induced T cell responses in Wuchereria bancrofti infections
Human lymphatic filariasis is a debilitating parasitic
disease characterized by downregulation of the host’s
immune response in asymptomatic carriers along with
profound hyperreactivity in chronic patients apart from
putatively immune endemic normals. The endosymbiont
Wolbachia, a bacterium of filarial nematodes has received
much attention as possible chemotherapeutic target and its
involvement in disease pathogenesis. The role of recombinant
Wolbachia surface protein (rWSP), one of the most
abundantly expressed proteins of the endosymbiont, in
modulating cell-mediated immune responses in patients
harboring Wuchereria bancrofti infections was evaluated in
the current study. rWSP-induced lymphoproliferation with
peripheral blood mononuclear cells suggested an impaired
proliferative response in asymptomatic microfilaremic (MF)
and symptomatic chronic pathology (CP) patients compared
to endemic normals (EN). This was further supported by a significantly diminished expression of CD69 along with
elevated levels of CD127 and CD62L in filarial patients
(MF and CP) compared to EN. Further, rWSP induced
the expression of regulatory T cell markers CTLA-4 and
CD25 along with suppressor cytokines IL-10 and TGF-β
in MF and CP patients compared to EN. However, the
rWSP-stimulated expression of IFN-Îł was diminished
significantly in filarial patients compared to endemic
normals. Thus, these findings suggest that WSP may also
contribute to the suppression of immune responses seen
in filarial patients
Macrophage and T cell produced IL-10 promotes viral chronicity
Chronic viral infections lead to CD8(+) T cell exhaustion, characterized by impaired cytokine secretion. Presence of the immune-regulatory cytokine IL-10 promotes chronicity of Lymphocytic Choriomeningitis Virus (LCMV) Clone 13 infection, while absence of IL-10/IL-10R signaling early during infection results in viral clearance and higher percentages and numbers of antiviral, cytokine producing T cells. IL-10 is produced by several cell types during LCMV infection but it is currently unclear which cellular sources are responsible for induction of viral chronicity. Here, we demonstrate that although dendritic cells produce IL-10 and overall IL-10 mRNA levels decrease significantly in absence of CD11c(+) cells, absence of IL-10 produced by CD11c(+) cells failed to improve the LCMV-specific T cell response and control of LCMV infection. Similarly, NK cell specific IL-10 deficiency had no positive impact on the LCMV-specific T cell response or viral control, even though high percentages of NK cells produced IL-10 at early time points after infection. Interestingly, we found markedly improved T cell responses and clearance of normally chronic LCMV Clone 13 infection when either myeloid cells or T cells lacked IL-10 production and mice depleted of monocytes/macrophages or CD4(+) T cells exhibited reduced overall levels of IL-10 mRNA. These data suggest that the decision whether LCMV infection becomes chronic or can be cleared critically depends on early CD4(+) T cell and monocyte/macrophage produced IL-10
CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells from cancer patients
Longitudinal Monitoring of the Development of Antifilarial Antibodies and Acquisition of Wuchereria bancrofti in a Highly Endemic Area of Haiti
Signatures of balancing selection in toll-like receptor (TLRs) genes – novel insights from a free-living rodent
Interference with PD-L1/PD-1 co-stimulation during antigen presentation enhances the multifunctionality of antigen-specific T cells
PC61 (Anti-CD25) Treatment Inhibits Influenza A Virus-Expanded Regulatory T Cells and Severe Lung Pathology during a Subsequent Heterologous Lymphocytic Choriomeningitis Virus Infection
IL-21 restricts virus-driven Treg cell expansion in chronic LCMV infection
Foxp3+ regulatory T (Treg) cells are essential for the maintenance of immune homeostasis and tolerance. During viral infections, Treg cells can limit the immunopathology resulting from excessive inflammation, yet potentially inhibit effective antiviral T cell responses and promote virus persistence. We report here that the fast-replicating LCMV strain Docile triggers a massive expansion of the Treg population that directly correlates with the size of the virus inoculum and its tendency to establish a chronic, persistent infection. This Treg cell proliferation was greatly enhanced in IL-21R-/- mice and depletion of Treg cells partially rescued defective CD8+ T cell cytokine responses and improved viral clearance in some but not all organs. Notably, IL-21 inhibited Treg cell expansion in a cell intrinsic manner. Moreover, experimental augmentation of Treg cells driven by injection of IL-2/anti-IL-2 immune complexes drastically impaired the functionality of the antiviral T cell response and impeded virus clearance. As a consequence, mice became highly susceptible to chronic infection following exposure to low virus doses. These findings reveal virus-driven Treg cell proliferation as potential evasion strategy that facilitates T cell exhaustion and virus persistence. Furthermore, they suggest that besides its primary function as a direct survival signal for antiviral CD8+ T cells during chronic infections, IL-21 may also indirectly promote CD8+ T cell poly-functionality by restricting the suppressive activity of infection-induced Treg cells