Comparative Microbial Analysis of Earthworm Casts Collected From Ikenne, Ogun State, Nigeria

A comparative assessment of the physicochemical parameters and microbial profile of three types of earthworm casts (Pellet, Mass and Turret) were determined. The total viable count (TVC), coliform counts, yeast and mould counts were determined using standard procedures. The pH ranged from 7.8 for Mass cast to 8.6 for Pellet cast. Moisture content of the casts ranged from 29.43% for pellet casts to 47.10% for turret casts. Organic matter was 1.05%, 0.72% and 0.63% for pellet, turret and mass casts respectively. TVC was 4.8 x 107cfu/g, 6.1 x 107cfu/g and 1.2 x 108cfu/g for Mass, Turret and Pellet casts respectively. Pellet cast recorded the highest coliform count (1.5 x 107) while Turret cast the least (7.3 x 106 cfu/g). Mould counts was 8.3 x 106 cfu/g for turret cast and 1.2 x 107cfu/g for pellet and mass casts. The lowest yeasts count was 1.2 x 107cfu/g in mass cast while the highest was 3.8 x 107cfu/g for pellet cast. Microbial distribution in the three casts types showed that Staphylococcus aureus is common to all Cast-types. Citrobacter spp, Pseudomonas fluorescens, Penicillium chrysogenum and S. rosei were isolated from Pellet cast only. Aspergillus fumigatus, Fusarium oxisporum, Pseudomonas aeruginosa and Penicillium oxalicum were isolated from only Turret Cast while Aspergillus terreus, Fusarium compacticum, Klebsiella aerogenes and Streptococcus feacalis were isolated from Mass Cast only. The presence of Bacillus licheniformis in Pellet Cast is an advantage plus for Pellet cast usage since this bacteria contributes to nutrient cycling and displays antifungal activities.Keywords: Pellet cast, Turret cast, Mass cast, Microbial Count

Comparative Analysis of the Growth Performance and Haemolymph Biochemical Properties of Normal and Albino Giant Land Snail- Archachatina marginata

This study was conducted to compare the growth performance and haemolymph metabolites concentrations of two snail ecotypes-normal skin and albino snail raised in captivity for eight weeks. The growth performance was measured by body weight gain, shell length gain and shell circumference gain  while the measured haemolymph metabolites were glucose, protein, lipids, Na+, K+ ,Ca2+, Cl- and PO42-. Normal snail recorded better growth performance and growth rate (3.432x) than albino snails (2.166x). Similarly significantly higher (P 0.05) was recorded in the concentrations of minerals of the two snails species haemolymph. The domestication of the two snail ecotypes is thus recommended for heliculturist.Keyword: Albino snails, Archachatina marginata, biochemical value,  haemolymph

Point-of-admission neonatal hypoglycaemia in a Nigerian tertiary hospital: incidence, risk factors and outcome.

Background: Neonatal hypoglycaemia is a major metabolic problem. It may result in mortality or severe handicap among survivors. Many babies admitted for neonatal care are at high risk for hypoglycaemia. The present study set out to determine its point-of-admission prevalence, clinical presentation and outcome. Methods: Consecutive neonates who met the study criteria had plasma glucose determined at admission into the special care baby unit of Wesley Guild Hospital. Hypoglycaemia was defined as plasma glucose of ≤ 2.5mmol/L. Babies with and without hypoglycaemia were compared for risk factors, clinical features and outcome. Results: A total of 150 neonates were studied out of which 49 (32.7%) had hypoglycaemia. The mean age, 38.3 ± 71.6 in hours was significant ly lower among neonates with hypoglycaemia than those without hypoglycaemia [p = 0.006].Lowsocioeconomic class (p = 0.034), admission weight less t h a n 2500g ( p = 0.009 ) , hypothermia (p = 0.001) and preterm birth (p = 0.020) were significantly more common in babies with hypoglycaemia. Poor suck (p = 0.010), cyanosis (p = 0.020), convulsion (p = 0.040) and pallor (p = 0.048) were also more common among babies with hypoglycaemia. The mortality rate in babies with hypoglycaemia was 32.7%, higher than 18.8% in babies without hypoglycaemia but the difference was not statistically significant (p = 0.060). Conclusion: Hypoglycaemia is common among high-risk neonates and is often associated with morbidity and mortality. Routine monitoring of blood glucose is therefore recommended for this class of babiesKeyWords: Prevalence, Point-of-admission , Neonatal Hypoglycaemia, Morbidity and Mortality, Nigeria

Effectiveness of an Oral Health Care Training Workshop for School Teachers: A Pilot Study

Background: School teachers play key roles in imparting appropriate and up-to-date knowledge to pupils and students. However, most teachers in developing countries like Nigeria have poor knowledge and motivation about oral health which may be due to inadequate training in the area of oral health. This might be one of the reasons for the poor oral hygiene among them and their students.Objectives: To evaluate the effectiveness of an oral health care training programme organized for teachers in Eruwa, Oyo state, Nigeria. Methods: An intervention study was conducted among 40 school teachers who attended a two day oral health training workshop at Eruwa, headquarters of Ibarapa East Local Government Area. Training methods included lectures and demonstrations on aetiology, clinical features, treatment and prevention of common oral diseases. Pre- and post- evaluation written tests were administered to the participants to assess the effectiveness of the training. The knowledge scores were rated as poor, fair and good knowledge scoring <50.0%, 50.0-60.0% and > 60.0% respectively. Frequencies, percentages and means of relevant variables were generated. Paired t-test was used to compare means at P<0.05.Result: The mean age of the teachers was 40.13 ±7.24 years. There were 16 (40.0%) males and 24 (60.0%) females. Twenty (50.0%) of the participants had poor pre-training knowledge as compared to 7 (17.5%) after the training. Thirteen (32.5%) had fair pre-training knowledge as compared to 17 (42.5%) post training. Only (2.5%) of the participants had good pre-training knowledge as compared to 18 (45.0%) post training. The mean scores of the pre- and post-evaluation tests were 31.70 ±11.31 and 48.20 ±11.16 respectively. There was a statistically significant difference between the mean scores of the pre and post evaluations P<0.05.Conclusions: The training workshop improved the knowledge of school teachers regarding oral health.Keywords: Effectiveness, Oral health training, Teachers

Changes in preterm birth and stillbirth during COVID-19 lockdowns in 26 countries

Preterm birth (PTB) is the leading cause of infant mortality worldwide. Changes in PTB rates, ranging from −90% to +30%, were reported in many countries following early COVID-19 pandemic response measures (‘lockdowns’). It is unclear whether this variation reflects real differences in lockdown impacts, or perhaps differences in stillbirth rates and/or study designs. Here we present interrupted time series and meta-analyses using harmonized data from 52 million births in 26 countries, 18 of which had representative population-based data, with overall PTB rates ranging from 6% to 12% and stillbirth ranging from 2.5 to 10.5 per 1,000 births. We show small reductions in PTB in the first (odds ratio 0.96, 95% confidence interval 0.95–0.98, P value <0.0001), second (0.96, 0.92–0.99, 0.03) and third (0.97, 0.94–1.00, 0.09) months of lockdown, but not in the fourth month of lockdown (0.99, 0.96–1.01, 0.34), although there were some between-country differences after the first month. For high-income countries in this study, we did not observe an association between lockdown and stillbirths in the second (1.00, 0.88–1.14, 0.98), third (0.99, 0.88–1.12, 0.89) and fourth (1.01, 0.87–1.18, 0.86) months of lockdown, although we have imprecise estimates due to stillbirths being a relatively rare event. We did, however, find evidence of increased risk of stillbirth in the first month of lockdown in high-income countries (1.14, 1.02–1.29, 0.02) and, in Brazil, we found evidence for an association between lockdown and stillbirth in the second (1.09, 1.03–1.15, 0.002), third (1.10, 1.03–1.17, 0.003) and fourth (1.12, 1.05–1.19, <0.001) months of lockdown. With an estimated 14.8 million PTB annually worldwide, the modest reductions observed during early pandemic lockdowns translate into large numbers of PTB averted globally and warrant further research into causal pathways

Changes in preterm birth and stillbirth during COVID-19 lockdowns in 26 countries.

Preterm birth (PTB) is the leading cause of infant mortality worldwide. Changes in PTB rates, ranging from -90% to +30%, were reported in many countries following early COVID-19 pandemic response measures ('lockdowns'). It is unclear whether this variation reflects real differences in lockdown impacts, or perhaps differences in stillbirth rates and/or study designs. Here we present interrupted time series and meta-analyses using harmonized data from 52 million births in 26 countries, 18 of which had representative population-based data, with overall PTB rates ranging from 6% to 12% and stillbirth ranging from 2.5 to 10.5 per 1,000 births. We show small reductions in PTB in the first (odds ratio 0.96, 95% confidence interval 0.95-0.98, P value <0.0001), second (0.96, 0.92-0.99, 0.03) and third (0.97, 0.94-1.00, 0.09) months of lockdown, but not in the fourth month of lockdown (0.99, 0.96-1.01, 0.34), although there were some between-country differences after the first month. For high-income countries in this study, we did not observe an association between lockdown and stillbirths in the second (1.00, 0.88-1.14, 0.98), third (0.99, 0.88-1.12, 0.89) and fourth (1.01, 0.87-1.18, 0.86) months of lockdown, although we have imprecise estimates due to stillbirths being a relatively rare event. We did, however, find evidence of increased risk of stillbirth in the first month of lockdown in high-income countries (1.14, 1.02-1.29, 0.02) and, in Brazil, we found evidence for an association between lockdown and stillbirth in the second (1.09, 1.03-1.15, 0.002), third (1.10, 1.03-1.17, 0.003) and fourth (1.12, 1.05-1.19, <0.001) months of lockdown. With an estimated 14.8 million PTB annually worldwide, the modest reductions observed during early pandemic lockdowns translate into large numbers of PTB averted globally and warrant further research into causal pathways

Short CoommunicationActivities of glycosidases in the foot muscles of African giant land snail, Archachatina marginata during aestivation

The growth performance and the activities of glycosidases (amylase, cellulase and α-glucosidase) in the foot muscle of giant land snail, Archachatna marginata were examined during aestivation. Aestivation significantly affected the growth performance of the snails as active snails gained 16.4±0.02g while the aestivated snails lost 15.5±0.1g. Three glycosidases were detected in the foot muscle of the snails at varying levels: 33-37 Abs/min (α-glucosidase), 11-15 Abs/min (amylase) and 28-31 Abs/min (cellulase) with the active snails having significantly higher activities (Abs/min) in α-glucosidase and cellulase. Aestivation thus significantly affects the foot muscle activities of A.marginata.Keywords/phrases: Aestivation, Glycosidases, Growth, SnailEthiop. J. Biol. Sci., 8(2): 165-170, 200

Prevention of neonatal tetanus: Much preached, less practiced!

Nigerian family with two consecutive perinatal deaths from neonatal tetanus is reported. The important role of missed opportunities in the failure of immunization programmes and the persistence of neonatal tetanus in the developing world is highlighted. Other important factors are also emphasised in order to reawaken a need for concerted effort towards the elimination of the disease.Keywords: immunization; missed opportunities; neonatal tetanus Nigerian Medical Practitioner Vol. 50 (2) 2006: pp 39-4

Influence of Different Management Systems on Gut Microbes and Chemical Constituents of Giant Land Snail (Archachatina marginata)

The impact of management systems on the African giant land snail, Archachatina marginata found in Abeokuta, Nigeria was investigated. The gut microbial load, haemolymph biochemical values (proteins, lipids, glucose, Na+, Ca2+,K+, CL- PO4 2+) and proximate composition (crude protein, fat, fibre, ash and carbohydrates) of the flesh were determined in these snails. There were significantly (p&lt;0.05) higher colony forming units (cfu) in the gut of snails from the wild (5-24 x103) than the domesticated snails (3-13 x103). The haemolymph biochemical values and flesh proximate composition were significantly higher in the snails from the wild than the domesticated ones. However, antinutrients and mineral composition of the flesh were not significantly affected by the management systems. The implication of these results on snail meat value in Nigeria is discussed.Keywords: Archachatina marginata, management systems, heliculture, gut microbe

An ethno-zoological survey of insects and their allies among the remos (Ogun State) South Western Nigeria

The research was carried out to study the socio-economic  characteristic of the Remos of Ogan State of Ngeria; to determine their relationship with insects which they regard as edible, which theyuse for rituals and which they use for medicinal purposes.Two hundred and eighty-five questionnaires were administered. The data collected was analysed by using frequency counts and percentages. Common edible insects were found to include termites(Macrotermes bellicossus), cricket (Brachytrypes sp) and (Analeptes trifaciata). The insects used for rituals and medicinal purposes include the butterfly used for making love portion; Cryptotheliarongeoti used as a defense against any evil; Brachytrypes sp, Lycosa sp and Apis melifera used for good fortune. The honey bee (Apis melifera) is used to prevent dizziness, while the sting of Ant is usedfor healing wound, curing of impotence in males and is also used as a cure for “thunderbolt”. The study found that in this community insects are valuable for both food and medicinal purposes. It recommends that more work on medicinal and nutritional value of these insects be undertaken