Selective Detection of H₂S by Copper Complex Embedded in Vesicles through Metal Indicator Displacement Approach

A new approach for the detection of hydrogen sulfide (H₂S) was constructed within vesicles comprising phospholipids and amphiphilic copper complex as receptor. 1,2-Distearoyl-<i>sn</i>-glycero-3-phosphocholine (DSPC) vesicles with embedded metal complex receptor (<b>1.Cu</b>) sites have been prepared. The vesicles selectively respond to H₂S in a buffered solution and show colorimetric as well as spectral transformation. Other analytes such as reactive sulfur species, reactive nitrogen species, biological phosphates, and other anions failed to induce changes. The H₂S detection is established through a metal indicator displacement (MIDA) process, where Eosin-Y (EY) was employed as an indicator. Fluorescence, UV–vis spectroscopy, and the naked eye as the signal readout studies confirm the high selectivity, sensitivity, and lower detection limit of the vesicular receptor. The application of vesicular receptors for real sample analysis was also confirmed by fluorescence live cell imaging