A new method for collecting isothiocyanates released from plant residues incorporated in soil

A method was developed for collecting isothiocyanates (ITCs) from compost and their detection by gas chromatography-mass spectrometry. Identification of the compounds was based on retention time, molecular weight and previously published information (Wiley Database, Hewlett Packard ChemStation Library). The release of ITCs from freeze-dried plant material of Brassica carinata, Cleome spinosa and Tropaeolum majus cultivars was investigated by incorporation in compost contained in pots. Allyl isothiocyanate, methyl isothiocyanate and benzyl isothiocyanate, respectively, were released after hydrolysis of plant tissues. Abundance of ITCs was monitored for 12 days and was shown to decrease rapidly following incorporation until the fourth day for all types of plant tissue. © 2007 Association of Applied Biologists