67 research outputs found
Vascular and apoptotic changes in the placode of myelomeningocele mice during the final stages of in utero development
JOAQUIM L. REIS, M.D., PH.D.,1,2 JORGE CORREIA-PINTO, M.D., PH.D.,3,4
MARIANA P. MONTEIRO, M.D., PH.D.,1 MADALENA COSTA, B.SC.,1
AND GROVER M. HUTCHINS, M.D.5
1Department of Anatomy, Abel Salazar Institute for the Biomedical Sciences and Unit for
Multidisciplinary Biomedical Research, University of Porto; 2Department of Neurosurgery, Santo
António General Hospital; 4Department of Pediatric Surgery, São João Hospital, Porto; 3Life and
Health Sciences Research Institute, School of Health Sciences, University of Minho, Braga, Portugal;
and 5Department of Pathology, Johns Hopkins School of Medicine, Baltimore, Maryland
Object. Myelomeningocele (MMC) is a primary neurulation defect that is associated with devastating neurological
disabilities in affected newborns. To better characterize the in utero neurodegenerative process of MMC, the authors
investigated the changes in vascular organization, apoptosis, and the presence of inflammatory cells during gestation
by using a mutant mouse model of MMC.
Methods. The curly tail/loop tail (ct/lp) mutant mouse model of MMC was chosen to obtain fetuses at different
stages of gestation. Mouse fetuses harboring MMC were harvested by caesarean section at embryonic Days 14.5, 16.5,
and 18.5 (complete mouse gestation at 19 days, 6 mice/group); littermate fetuses with the same gestational age but
without an MMC were used as controls. Samples of the MMC placode or normal spinal cord were stained for immunocytochemical
labeling with caveolin antibody (endothelium marker) and activated caspase-3 antibody (apoptosis marker).
Samples were morphometrically analyzed with a computer-assisted image analyzer.
Results. The MMC mice presented with an increase in vascular density from embryonic Days 16.5–18.5 and an
enhanced number of apoptotic cells at embryonic Day 18.5, compared with controls. There were scarce signals of an
inflammatory reaction in the MMC placode, as a few infiltrating neutrophils were seen only at embryonic Day 18.5.
Conclusions. Fetal placodes in MMC mice showed evidence of increased vascular density since embryonic Day
16.5 and increased apoptosis at embryonic Day 18.5. These new data support the view that in utero changes of the
MMC placode, occurring during the last stages of gestation, contribute to the neuropathological manifestations in fullterm
newborns with MMC. (DOI: 10.3171/PED/2008/2/8/150
Echocardiographic Diagnosis of Extrapericardial Tamponade Due to Dilated Gastric Roll following Oesophagectomy
Noninvasive measurement of shortening in the fiber and cross-fiber directions in the normal human left ventricle and in idiopathic dilated cardiomyopathy
Relationship of regional cross-fiber shortening to wall thickening in the intact heart: 3-D strain analysis using NMR tagging
This thesis explores social giving in the past century by looking at Andrew Carnegie and his influence on philanthropy and on the American business, TOMS, that integrates giving into its corporate structure. This historical research provides a conceptual context for the small business I created in August 2012. My business, Double Vision, applies the ideas of corporate social responsibility on a small scale to impact a community in the Dominican Republic. Included in the paper is an overview of the progression of social giving in the past century through the focus on Andrew Carnegie and TOMS, an analysis of the benefits and detriments of philanthropic giving, and my personal application. The purpose of this project is to increase my knowledge and understanding of corporate social responsibility and apply the ideas to a personal business venture
Quantification of principal strains in the normal human left ventricle using tagged magnetic resonance imaging in 3D
Relationship of regional cross-fiber shortening to wall thickening in the intact heart: 3-D strain analysis using NMR tagging
Pathology of the intima in coarctation of the aorta: a study using light and scanning electron microscopy
Assessment of the severity of coronary artery disease at postmortem examination. Are the measurements clinically valid?
A correlation of radiographic, functional, and morphologic changes in baboon lung allografts
- …