13 research outputs found
Induction of defense responses in common bean plants by arbuscular mycorrhizal fungi
Interaction between arbuscular mycorrhizal fungi as a bio-agent and Rhizoctonia root rot disease of common bean plant was investigated in this study under natural conditions in pot experiment. A mixture of Egyptian formulated AM (Multi-VAM) in suspension form (1x10(6)unitL(-1) in concentration) was used at dilution of 5mlL(-1) water. The results demonstrated that colonization of bean plants with AM fungi significantly increased growth parameters, yield parameters and mineral nutrient concentrations and reduced the negative effects on these parameters as well as both disease severity and disease incidence. Different physical and biochemical mechanisms have been shown to play a role in enhancement of plant resistance against Rhizoctonia solani, namely, improved plant nutrition, improved plant growth, increase in cell wall thickening, cytoplasmic granulation, and accumulation of some antimicrobial substances (phenolic compounds and defense related enzymes)
Chemical Compositions and Antiproliferative Effect of Citrus sinensis and Citrus aurautium Flowers in The Nest Anatolian Ecological Conditions
The aim of this was to assessment chemical composition,
antiproliferative of valuable essential oils from Citrus sinensis and
Citrus aurantium flowers commonly used in Anatolia. According to the
results of the gas chromatography-mass spectrometry analysis, nerolidol
(22.13 \%), linalool (14.06 \%) were found to be highest in the
essential oil obtained from C. sinensis. Nerolidol (28.07 \%),
2,6,10-dodecatriene-1-ol (15.11 \%), were found to be highest in the
essential oil obtained from C. aurantium. The antiproliferative activity
for both natural products was conducted following the European
Pharmacopoeia 8.0 protocol. According to this protocol, we in both
products for in vitro antiproliferative activity against HeLa, HT29,
A549, Hep3B, MCF7 cancer cells and FL normal cells using a cell
proliferation assay, cytotoxicity assay (LDH), and phase-contrast
microscopic image evaluation techniques. Both essential oils exhibited
the strong antiproliferative effect together with low cytotoxicity
values at the low and mid concentration (1.9-2.9 Log(10) mu g/mL). Both
samples altered the morphological shape of the cells from being cell
shrinkage and rounding to being decreased cell elongation and volume at
high concentration (2.9-4.4 Log(10) mu g/mL). However, these essentials
oils caused high cytotoxic effect against FL normal cells compared to
that of cancer cells, indicating that these were not promising suitable
antiproliferative agents against cells tested. We believe that both
essential oils need to be developed by making a more rational research
because they have affected normal FL cells. As a conclusion, it seems
substantial to continue to improve both essential oils to help the
cancer problem further