14 research outputs found
A comprehensive overview of substrate specificity of glycoside hydrolases and transporters in the small intestine
No full textAntibodies against synthetic oligosaccharide antigens reactive with extracellular polysaccharides produced by moulds.
No full textA rapid and reliable method for the detection of molds in foods: using the latex agglutination assay.
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Specific recognition of antibody-oligonucleotide conjugates by radiolabeled antisense nucleotides : a novel approach for radio-immunotherapy of cancer
No full textAbstract in Articl
Use of a synthetic DNA oligonucleotide to probe the precision of RNA splicing in a yeast mitochondrial petite mutant
No full textA human gastric carcinoma contains a single mutated and an amplified normal allele of the Ki- ras
No full textA* protein of bacteriophage [phi]X174 carries an oligonucleotide which it can transfer to the 3-OH of a DNA chain
Get PDFThe bacteriophage φX174 gene A encodes two proteins: gene A protein and A* protein. Purified A* protein acts as a single-stranded, DNA-specific endonuclease which remains covalently attached to the 5′-end of the cleavage site. Incubation of A* protein with the synthetic heptamer CAACTTG or with oligonucleotides which yield this heptamer after cleavage with the A* protein yields oligonucleotides with the sequences CAACTTGAG, CAACTTGAGG and CAACTTGAGGA. This indicates that A* protein carries an oligonucleotide with the sequence -AG, AGG or -AGGA. The oligonucleotide can be transferred to the 3′-end of the heptamer CAACTTG. This suggests that A* protein reacts with a specific DNA sequence in the infected cell.
Author Keywords: Bacteriophage φX174; A* protein; DNA—protein complex; Synthetic oligonucleotide; DNA-ligating activit
Restoration of the biological activity of in vitro synthesized øX DNA by transfection of ung— spheroplasts or dUTPase treatment
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