Functional reserves of students related to a special medical group for health reasons

The relevance of this work is determined by the high prevalence of physical inactivity among students assigned to a special medical group for health reasons. The purpose of the study is to analyze the impact of physical education in a special medical group on the functional reserves of students’ body. A group of 20 first-year students was observed. Girls were engaged in physical culture during one school year. The examination at the beginning and end of the observation period included anthropometry, physiometry and functional testing. The heart rate and respiration rate in most girls exceeded the upper limit of the physiological norm; the vital capacity of the lungs did not reach the lower limit of the norm during initial testing. Functional tests showed low results. Statistically significant decrease heart rate and respiration rate increasing the lung capacity was found on re-examination. The results of the functional test have improved. The results obtained led to the conclusion that body’s functional reserves are reduced in students assigned to a special medical group for health reasons. Feasible physical culture classes are accompanied by the expansion of the body’s functional reserves. &nbsp

Effects of experimental <i>Trichinella spiralis</i> larvae infection in rats and mice on hematological and biochemical parameters of the host at different infection stages

The purpose of the research is the study of effects of experimental Trichinella spiralis infection in rats and mice on hematological and biochemical blood parameters of the host at different infection stages.Materials and methods. The experiment was performed on mature outbred male rats and male mice that were infected by T. spiralis larvae orally administered at the rate of 10 larvae/1 g of body weight. Blood samples were taken from the rats at 40 days and 3 months after infection; from the mice, on day 40 after infection. The main parameters of peripheral blood in the rats were determined by a MicroCC-20 Plus hematological analyzer (High Technology, Inc. (USA)) using reagents from Clinical Diagnostic Solutions, LLC (Russia). Biochemical blood parameters in the rats and mice were determined by a Clima MC-15 analyzer, RAL Technical el Laboratoria, S.A. (Spain) using reagents produced by Diakon-DS, CJSC (Russia).Results and discussion. At 40 days and 3 months after the rats were experimentally infected with T. spiralis, we observed a consistent increase in the hemoglobin concentration, erythrocytes and leukocytes, and hematocrit, and a decrease in the alkaline phosphatase activity and ALT, and an increase in total protein. On day 40 after infection in the mice, an increase in total protein was observed as in the rats, and a decrease in blood urea. The changes detected in hematological and biochemical parameters in the rats and mice were associated with the toxic effect of Trichinella infection and the response of the host organism. Such changes are dependent on the infection stage and animal species

SOME WAYS OF PRODUCTION PROCESS OF LIVE PLAGUE VACCINE AND TECHNIQUES OF EVALUATION THE IMMUNOGENICITY (REVIEW)

The modern findings of scientific research in a live plague vaccine range such as production antibiotic-resistant subcultures of the vaccine strains, design of the low-development of reaction producers vaccine strains, production of oral (tablet) preparation are represented in the review. Some techniques of production improvement live plague vaccine are formulated: stabilization of a number alive microbial cells in a inoculation dose of a license vaccine by decreasing of cultivation temperature mode from 27 °C till 21°С, concentration of microbial cells in ampoule from (5-10) × 1010 till (1-4) × 1010 per 1 ml, reduction freezing-drying step from 28 till 18 h, decreasing of amount dose in ampoule from 100-125 till 20-50 and furthermore acceleration of production process of microbial cells concentrate (fermentation in the bioreactor, tangential microfiltration) and formulation combined (meat-soya nutritious base) medium and conditions of their preparation (time reduction enzymatic hydrolysis from 14 till 3 days). The tendenceis of refinement of a technological process of equipment link were researched, such as using bioreactors instead of apparatusfor a cultivation microbesShesterenko, disposable bags Flexboy, which are allowed to eliminate the application of glass bottles and operation of a bottle assembling, also using of robotizing sublimation installations for drying of a liquid form of live plague vaccine, which are allowed to exclude utilization of low-temperature refrigerators. Beside of regulating estimation method of immunogenicity (calculation of effective immunizing dose) and quantitative account of a phenomenon of survival white mice the cyto-enzymochemical and morphometric rates are suggested to estimate the quality of ultimate product live plague dry vaccine, changes of those are charactering immunologic activity and state of mobilizing and bactericidal abilities of a organism phagocyte system cells

LITHOLYTIC THERAPY AT THE TREATMENT OF CHOLELITHIASIS (REVIEW OF LITERATURE)

Treatment of cholelithiasis in patients with high operative risk need requires minimally invasive methods. Contact bilestones litholysis is a perspective direction. For this purpose it is recommended to use medications with the highest litholytic activity and the lowest side effects. It is necessary to seek for an adequate concrement dissolvent because of mineral and organic composition of bilestones. The article presents comparative analysis of characteristics of various dissolving agents proceeding from effectiveness and intensity toxic effects

Modification of low-density lipoproteins by low molecular weight carbonyl products of free-radical oxidation of lipids and carbohydrates plays a key role in atherosclerotic lesion of the vascular wall and in endothelial dysfunction

The review presents evidence of the participation of low-density lipoproteins (LDL) modified by low molecular weight dicarbonyl compounds formed during freeradical oxidation of lipids (malondialdehyde) and carbohydrates in the development of endothelial dysfunction and atherosclerotic vascular lesions. The authors believe that it is they, and not oxidized (hydroperoxide-containing) LDL, that are the main factors of pathogenesis. The role of dicarbonyl-modified LDL in LOX-1 dependent induction of processes leading to the development of endothelial dysfunction is discussed. The results of studies proving that damage to the glycocalyx (a layer of macromolecules that prevent the development of endothelial dysfunction) covering the luminal surface of the endothelium is caused by hyperproduction of reactive oxygen species. Ways of pharmacological correction of free-radical oxidation processes are discussed, due to which inhibition of atherogenesis and diabetogenesis can be achieved

Adaptive potential of spring durum wheat (<i>Triticum durum</i> Desf.) under the conditions of Primorsky Territory

Background. The research goal was to study spring durum wheat cultivars from different regions of Russia to select those that were most adapted to the conditions of Primorsky Territory and had high yield and grain quality.Materials and methods. The adaptive potential of 16 genotypes was determined through multi-environment trials at the Federal Scientific Center of Agricultural Biotechnology of the Far East named after A.K. Chaika in 2017–2021.Results. Considerable potential for high productivity was revealed in the cultivars: ‘Solnechnaya 573’ (3.9 t/ha), ‘Donskaya elegiya’ (3.8 t/ha), ‘Lyudmila’ (3.8 t/ha), ‘Omskii izumrud’ (3.8 t/ha), and ‘Voronezhskaya 7’ (3.8 t/ha). Cvs. ‘Pamyati Yanchenko’ (–2.1), ‘Zhemchuzhina Sibiri’ (–2.4), and ‘Voronezhskaya 7’ (–2.4) displayed significant stress resistance. ‘Lyudmila’ (4.2) and ‘Donskaya elegiya’ (4.0) had high genetic plasticity and produced high yields in the years with favorable conditions. The adaptability coefficient of cvs. ‘Solnechnaya 573’ (112.5%) and ‘Voronezhskaya 7’ (111.0%) exceeded 100%. ‘Donskaya elegiya’, ‘Lyudmila’, ‘Aleiskaya’, and ‘Voronezhskaya 9’ demonstrated the highest plasticity (bi &gt; 1). The most stable cultivars were ‘Omskii tsirkon’ (S2di – 0.00), ‘Pamyati Yanchenko’ (S2di – 0.02), ‘Voronezhskaya 7’ (S2di – 0.04), ‘Aleiskaya’ (S2di – 0.05), ‘Nikolasha’ (S2di – 0.06), and ‘Omskaya biryuza’ (S2di – 0.08). ‘Voronezhskaya 7’ and ‘Pamyati Yanchenko’ were able to produce an increased yield and had the minimum yield loss under unfavorable conditions (the indicator of the yield stability of the cultivars was 134.9 and 144.8, respectively). ‘Pamyati Yanchenko’ (4.8 and 1.9, respectively) and ‘Voronezhskaya 7’ (3.5 and 2.0, respectively) showed high homeostatic capacity and breeding value.Conclusions. ‘Voronezhskaya 7’ and ‘Solnechnaya 573’ were selected on the basis of complex evaluation for yield and adaptability under the local conditions. They can be recommended for further breeding and production

1-(8-Bromo-2-methyl-4-thioxo-3,4,5,6-tetra­hydro-2H-2,6-methano-1,3-benzoxazocin-11-yl)ethanone

In the title compound, C14H14BrNO2S, there are two similar non-equivalent mol­ecules in the asymmetric unit, displaying three chiral centres each. In the crystal structure, they are linked by inter­molecular N—H⋯O hydrogen bonds to form infinite chains, which are in turn connected by weak Br⋯H and S⋯H inter­actions

Reactions of 2-(5-methyl-2-phenyl-2H-1,2,3-diazaphosphol-4-yl)- 4H-1,3,2-benzodioxaphosphinin-4-one with chloral and hexafluoroacetone

Reaction of 2-(5-methyl-2-phenyl-2H-1,2,3-diazaphosphol-4-yl)-4H-1,3,2- benzodioxaphosphinin-4-one with chloral occurs at Piii atom of the 1,3,2-dioxaphosphinine cycle giving mostly 2-chlorocarbonylphenyl 2,2-dichlorovinyl (5-methyl-2-phenyl-2H-1,2,3-diazaphosphol-4-yl)phosphonate, whereas hexafluoroacetone incorporates into the 1,3,2-dioxaphosphorine cycle affording the corresponding 1,3,2-benzodioxaphosphepine. © 2011 Mendeleev Communications. All rights reserved

Влияние экспериментального заражения крыс и мышей личинками Trichinella spiralis на гематологические и биохимические показатели у хозяина на разных стадиях инвазии

The purpose of the research is the study of effects of experimental Trichinella spiralis infection in rats and mice on hematological and biochemical blood parameters of the host at different infection stages.Materials and methods. The experiment was performed on mature outbred male rats and male mice that were infected by T. spiralis larvae orally administered at the rate of 10 larvae/1 g of body weight. Blood samples were taken from the rats at 40 days and 3 months after infection; from the mice, on day 40 after infection. The main parameters of peripheral blood in the rats were determined by a MicroCC-20 Plus hematological analyzer (High Technology, Inc. (USA)) using reagents from Clinical Diagnostic Solutions, LLC (Russia). Biochemical blood parameters in the rats and mice were determined by a Clima MC-15 analyzer, RAL Technical el Laboratoria, S.A. (Spain) using reagents produced by Diakon-DS, CJSC (Russia).Results and discussion. At 40 days and 3 months after the rats were experimentally infected with T. spiralis, we observed a consistent increase in the hemoglobin concentration, erythrocytes and leukocytes, and hematocrit, and a decrease in the alkaline phosphatase activity and ALT, and an increase in total protein. On day 40 after infection in the mice, an increase in total protein was observed as in the rats, and a decrease in blood urea. The changes detected in hematological and biochemical parameters in the rats and mice were associated with the toxic effect of Trichinella infection and the response of the host organism. Such changes are dependent on the infection stage and animal species.Цель исследований – изучение влияния экспериментального заражения крыс и мышей Trichinella spiralis на гематологические и биохимические показатели крови у хозяина на разных стадиях инвазии.Материалы и методы. Опыт проводили на половозрелых аутбредных крысах-самцах и мышах-самцах, которых заражали пероральным введением личинок T. spiralis из расчета 10 личинок/1 г массы тела. Пробы крови отбирали у крыс через 40 сут и 3 мес. после заражения; у мышей – на 40-е сутки после заражения. Основные показатели периферической крови крыс определяли на гематологическом анализаторе «MicroCC-20 Plus» («High Technology, Inc.» (США)) с использованием реактивов ООО «Клиникал Диагностик солюшнз» (Россия). Биохимические показатели крови крыс и мышей определяли на анализаторе «Clima MC-15», RAL Technical el Laboratoria, S.A. (Испания) с использованием реактивов производства ЗАО «Диакон-ДС» (Россия).Результаты и обсуждение. Через 40 сут и 3 мес. после экспериментального заражения крыс T. spiralis отмечали последовательное повышение концентрации гемоглобина, числа эритроцитов и лейкоцитов и уровня гематокрита, снижение активности щелочной фосфатазы и АЛТ, а также повышение уровня общего белка. На 40-е сутки после заражения у мышей наблюдали повышение содержания общего белка, как и у крыс, а также снижение уровня мочевины в крови. Выявленные изменения гематологических и биохимических показателей у крыс и мышей связаны с токсическим влиянием трихинеллезной инвазии и ответной реакцией организма хозяина на нее. Изменения носят зависимый от стадии инвазии и вида животных характер