Phytoestrogen Secoisolaciresinol Diglicosídeo changes levels the estradiol and relative body of ovariectomized female rats Wistar

Aims: To evaluate the influence of phytoestrogen Secoisolaciresinol diglicosídeo flaxseed over a long time period and its hormonal effects on weight loss in castrated rats.  Materials and Methods: Wistar rats 21 that underwent bilateral oophorectomy for induction of menopause were randomly divided into three groups: Control group (n=7) received diet based on 10% casein, Flaxseed group (n=7) received flaxseed diet based on casein plus 25% flaxseed and the Modified control group (n=7) received modified diet based on 10% casein with the addition of lipid and fiber. All diets were prepared according to the recommendations of the AIN 93, and the animals received water ad libitum. The rats were anesthetized with Thiopentax® for blood collection by cardiac puncture to determine 17β-estradiol. The acquired data were statistically analyzed by the nonparametric Kruskal-Wallis test and post hoc Mann-Whitney U for comparison two by two through Software S-Plus version 8.0, with a 0.05 level for the significant differences. Results: At the end of 180-day experiment we observed that the results suggest that SDG is present in flaxseed at 13.5 ± 0.4 mg/g, feed intake in FG (1641.1 ± 0.9), CG (1478.4 ± 0.1) and MCG (1605.8 ± 1.8) showed no significant difference between groups. Have the mass on animals body it is observed that the FG (275.5 ± 33.9), CG (294.0 ± 33.1) and MCG (339.5 ± 24.6), suggesting that was no statistical difference between groups (p<0.002). Serum levels of 17β-estradiol showed higher values in FG (48.7 ± 12.31 pg/dl), GC (29.0 ± 5.4 pg/dl), MCG (36.0 ± 5.60 pg/dl), with statistical differences (p = 0.0012). Conclusion: The consumption of Secoisolaciresinol Diglicosídeo in food intake does not influence on body mass of animals and levels of estradiol in ovariectomized rats. RESUMOFitoestrógeno Secoisolaciresinol Diglicosídeo altera níveis de 17β estradiol e peso relativo corporal de ratas ooforectomizadasObjetivo: avaliar a influência do fitoestrógeno Secoisolaciresinol Diglicosídeo por um longo período de tempo e os efeitos hormonais sobre a perda de peso em ratas castradas. Materiais e Métodos: 21 ratas Wistar submetidas à ooforectomia bilateral para indução da menopausa, divididas aleatoriamente em três grupos: grupo controle recebeu dieta à base de caseína a 10%, Grupo linhaça dieta acrescido de 25% de linhaça e grupo controle modificado dieta modificada à base de caseína 10% com a adição de lipídios e fibras.   Para realização da ooforectomia as ratas foram anestesiadas com Thiopentax® e para determinar o 17β-estradiol no soro dos animais foi realizada coleta de sangue por punção cardíaca. Os dados obtidos foram analisados estatisticamente através do teste não paramétrico Kruskal-Wallis e post hoc de Mann-Whitney para comparar dois a dois através de um software versão S-Plus 8.0, com um nível de 0,05 para diferenças significativas. Resultados: Ao final de 180 dias de experimento os resultados apresentaram que o SDG está presente na semente de linhaça 13,55 ± 0,4 mg/g, o consumo do GL (1641,1 ± 0,9), GC (1478,4 ± 0,1) e GCM (1605,8 ± 1,8), não mostraram diferença significativa entre os grupos. A massa corporal relativa dos animais apresentou no GL (275,5 ± 33,9), GC (294,0 ± 33,1) e GCM (339,5 ± 24,6) diferenças estatísticas entre os grupos (p<0,002), os níveis séricos de 17ß-estradiol apresentaram o GL (48,7 ± 12,31 pg/dl), GC (29,0 ± 5,4 pg/dl), GCM (36,0 ± 5,60 pg/dl), com diferença estatística entre os grupos (p=0,0012). Conclusão: Secoisolaciresinol Diglicosídeo não influenciou no consumo alimentar, influenciando no peso relativo corporal e níveis de estradiol das ratas ovariectomizadas

Exposure to flaxseed during lactation does not alter prostate area or epithelium height but changes lipid profile in rats Exposición a la linaza durante la lactación no cambia el área o altura epitelial de la próstata pero cambia el perfil lipídico en ratones

Flaxseed intake has increased owing to beneficial effects to health and prevention of diseases. Provided that it's an important source of lignan, a phytoestrogen, the present study aimed at evaluating the possible effect of the intake of this seed during lactation upon prostate, sexual hormones and lipidic profile of the offspring in adult life. Material and methods: 16 female Wistar rats were used. After delivery, they were divided into two different groups to receive one of the following diets during lactation: Control group (CG), with a casein based diet and Flaxseed group (FG), with a flaxseed based diet containing 25% flaxseed. At weaning, male pups received commercial chow until adult life (170 days old), when they were sacrificed. Results: No differences were perceived concerning offspring food intake and body weight at 170 days. There was a reduction in total cholesterol levels (FG = 45.71 &plusmn; 8.96 mg/dL; CG = 63.43 &plusmn; 15.69 mg/dL, p = 0.02) and triglycerides (FG = 54.29 &plusmn; 11.10 mg/dL; CG = 79.86 &plusmn; 25.68 mg/dL, p = 0.03). Also, no alterations were observed in prostatic morphology, testosterone or estradiol levelsin the two groups analyzed. Conclusion: Flaxseed intake during lactation did not produce histological alterations in prostatic alveolus or in sexual hormones, but programmed to a reduction in lipid profile in adult life with decreased cardiovascular risk.El consumo de la linaza ha aumentado debido a los efectos beneficiosos para la salud y la prevención de enfermedades. Siendo una importante fuente de lignanos, un fitoestrógeno, el presente estudio evaluó los efectos de la administración de esta semilla durante la lactación en la próstata, las hormonas sexuales y perfil lipídico de loshijos en la vida adulta. Material y métodos: Fueron utilizados 16 ratones Wistar hembras. Después del parto fueron divididas en dos grupos recibiendo durante la lactación las siguientes dietas: Grupo Control (GC), con ración a base de caseína y Grupo Linaza (GL), con ración a base de caseína conteniendo 25% de semilla de linaza. En el destete, las crías machos pasaron a recibir ración comercial hasta la edad adulta, cuando fueron muertos a los 170 días de vida. Resultados: No fueron verificadas diferencias sobre el consumo alimentario y peso corporal de los animales a los 170 días. Hubo una reducción en los niveles de colesterol total (GL = 45,71 &plusmn; 8,96 mg/dL; GC = 63,43 &plusmn; 15,69 mg/dL, p = 0,02) y triglicéridos (GL = 54,29 &plusmn; 11,10 mg/dL; GC = 79,86 &plusmn; 25,68 mg/dL, p = 0,03). Además, no se observaron alteraciones en la morfología de la próstata, la testosterona o los niveles de estradiol en los dos grupos analizados. Conclusión: La administración de la semilla durante la lactación no promueve alteraciones histológicas en los alvéolos de próstata o en las hormonas sexuales, pero programado para una reducción en el perfil lipídico en la vida adulta con una disminución del riesgo cardiovascular