NGF-response of EGF-dependent progenitor cells obtained from human sympathetic ganglia

SIGNALLING molecules are thought to play a significant role in determining the fate of neural crest progenitor cells. The human sympathetic chain was identified at 6.5, 7.5, 8.2, 10.2 and 11.4 postconception (PC) weeks demonstrating low affinity nerve growth factor (NGF) receptors, and was processed for tissue culture. In the presence of epidermal growth factor (EGF), floating spheres of proliferating progenitor cells were developed in vitro. In the absence of EGF progenitor cells differentiated into tyrosine hydroxylase (TH)-immunoreactive neuronal and TH-negative flat cells. NGF treatment significantly increased neurite outgrowth and survival of TH-immunoreactive cells. The multipotent cells we isolated differ from previously reported sympathoadrenal progenitors in that they give rise to TH immunoreactive neurones precociously sensitive to NGF

Seeing into the past: integrating 3D documentation and non-invasive prospecting methods for the analysis, understanding and reconstruction of the ancient Pompeii. The case of the House of Obellio Firmo (IX, 14)

In 2015 the Department of History and Cultures of the Bologna University took part in the Grande Progetto Pompei - Piano della Conoscenza, with the task of providing a modern and complete documentation of the so-called Lotto 3 in Pompeii. The new survey was carried out by means of integrated innovative diagnostic survey techniques in order to provide a total documentary research of the whole sector. In 2016 a new project was started in agreement with the competent Superintendency, and focused on the study and preservation of the House of Obellio Firmo, included in the Lotto 3 of the Roman city. The new research contemplates an in-depth analysis of the building, employing systematic laser scanning and photogrammetry methods to generate an accurate 3D model of the house. This model is going to constitute the starting point for the further analysis of the wall stratigraphies and for the mapping and monitoring of the structures’ state of decay. The full-scale analytical documentation of the building also includes a detailed geophysical mapping of all the accessible domestic spaces, by using the ground penetrating radar technique. The preliminary results achieved by the non-invasive prospecting survey, integrated with the analysis of the surviving walls and building techniques, supply valid information for the archaeological interpretation of the house’s history. In order to allow the management and sharing of the information collected, the data are going to be organised within a building information model (BIM) with a triple objective: the reconstruction of a fragment of the ancient urban landscape in Pompeii during the oldest phase, with particular attention directed to the Samnitic period; the outlining of a precise strategy of intervention for the restoration and preservation of the House of Obellio Firmo; the re-opening of the building to sightseeing tours and its restitution to public use

Noninvasive near-infrared live imaging of human adult mesenchymal stem cells transplanted in a rodent model of Parkinson’s disease

Background: We have previously shown that human mesenchymal stem cells (hMSCs) can reduce toxin-induced neurodegeneration in a well characterized rodent model of Parkinson's disease. However, the precise mechanisms, optimal cell concentration required for neuroprotection, and detailed cell tracking need to be defined. We exploited a near-infrared imaging platform to perform noninvasive tracing following transplantation of tagged hMSCs in live parkinsonian rats.Methods: hMSCs were labeled both with a membrane intercalating dye, emitting in the near-infrared 815 nm spectrum, and the nuclear counterstain, Hoechst 33258. Effects of near-infrared dye on cell metabolism and proliferation were extensively evaluated in vitro. Tagged hMSCs were then administered to parkinsonian rats bearing a 6-hydroxydopamine-induced lesion of the nigrostriatal pathway, via two alternative routes, ie, intrastriatal or intranasal, and the cells were tracked in vivo and ex vivo using near-infrared technology.Results: In vitro, NIR815 staining was stable in long-term hMSC cultures and did not interfere with cell metabolism or proliferation. A significant near-infrared signal was detectable in vivo, confined around the injection site for up to 14 days after intrastriatal transplantation. Conversely, following intranasal delivery, a strong near-infrared signal was immediately visible, but rapidly faded and was completely lost within 1 hour. After sacrifice, imaging data were confirmed by presence/absence of the Hoechst signal ex vivo in coronal brain sections. Semiquantitative analysis and precise localization of transplanted hMSCs were further performed ex vivo using near-infrared imaging.Conclusion: Near-infrared technology allowed longitudinal detection of fluorescent-tagged cells in living animals giving immediate information on how different delivery routes affect cell distribution in the brain. Near-infrared imaging represents a valuable tool to evaluate multiple outcomes of transplanted cells, including their survival, localization, and migration over time within the host brain. This procedure considerably reduces the number of animal experiments needed, as well as interindividual variability, and may favor the development of efficient therapeutic strategies promptly applicable to patients

Inefficient skeletal muscle oxidative function flanks impaired motor neuron recruitment in Amyotrophic Lateral Sclerosis during exercise

This study aimed to evaluate muscle oxidative function during exercise in amyotrophic lateral sclerosis patients (pALS) with non-invasive methods in order to assess if determinants of reduced exercise tolerance might match ALS clinical heterogeneity. 17 pALS, who were followed for 4 months, were compared with 13 healthy controls (CTRL). Exercise tolerance was assessed by an incremental exercise test on cycle ergometer measuring peak O2 uptake ([Formula: see text]O2peak), vastus lateralis oxidative function by near infrared spectroscopy (NIRS) and breathing pattern ([Formula: see text]E peak). pALS displayed: (1) 44% lower [Formula: see text]O2peak vs. CTRL (p\u2009<\u20090.0001), paralleled by a 43% decreased peak skeletal muscle oxidative function (p\u2009<\u20090.01), with a linear regression between these two variables (r2\u2009=\u20090.64, p\u2009<\u20090.0001); (2) 46% reduced [Formula: see text]Epeak vs. CTRL (p\u2009<\u20090.0001), achieved by using an inefficient breathing pattern (increasing respiratory frequency) from the onset until the end of exercise. Inefficient skeletal muscle O2 function, when flanking the impaired motor units recruitment, is a major determinant of pALS clinical heterogeneity and working capacity exercise tolerance. CPET and NIRS are useful tools for detecting early stages of oxidative deficiency in skeletal muscles, disclosing individual impairments in the O2 transport and utilization chain

Neurology and the COVID-19 emergency

Following the COVID-19 outbreak, significant changes have been implemented on a national level in the organization of neurology units and associated stroke units. Regionallydesignated COVID-19 hospitals have implemented an aggressive policy to relocate as many beds as possible to COVID-19 patients. In order to do so, the preferred strategy has been to reduce the number of beds in neurology units, and in some cases several units have been consolidated into one. In other cases, particularly in the northern regions