730 research outputs found

    Maximum likelihood estimation of stochastic volatility models

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    This paper discusses the Monte Carlo maximum likelihood method of estimating stochastic volatility (SV) models. The basic SV model can be expressed as a linear state space model with log chi-square disturbances. The likelihood function can be approximated arbitrarily accurately by decomposing it into a Gaussian part, constructed by the Kalman filter, and a remainder function, whose expectation is evaluated by simulation. No modifications of this estimation procedure are required when the basic SV model is extended in a number of directions likely to arise in applied empirical research. This compares favorably with alternative approaches. The finite sample performance of the new estimator is shown to be comparable to the Monte Carlo Markov chain (MCMC) method

    Stress-related differential expression of multiple β-carotene ketolase genes in the unicellular green alga Haematococcus pluvialis

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    The unicellular green alga Haematococcus pluvialis is used as a biological production system for astaxanthin. It accumulates large amounts of this commercially interesting ketocarotenoid under a variety of environmental stresses. Here we report the identification and expression of three different β-carotene ketolase genes (bkt) that are involved in the biosynthesis of astaxanthin in a single strain of the alga. Bkt1 and bkt2 proved to be the crtO and bkt previously isolated from two different strains of H. pluvialis. Bkt3 is a novel third gene, which shared 95% identical nucleotide sequence with bkt2. Nitrogen deficiency alone could not induce the alga cells to produce astaxanthin in 3 days even though it enhances the expression of the bkt genes to three times of that in normal growing cells within 16 h. High light irradiation (125 μmol m -2 s -1) or 45 mM sodium acetate greatly increased the expression of bkt genes to 18 or 52 times of that in normal growing cells, resulting in an accumulation of substantial astaxanthin (about 6 mg g -1 dry biomass) in 3 days. It is suggested that the existence of the multiple bkt genes and their strong up-regulation by different stress conditions is one of the reasons that H. pluvialis accumulates large amounts of astaxanthin in an instant response to stress environments. © 2005 Elsevier B.V. All rights reserved.postprin

    Engineered maize as a source of astaxanthin:processing and application as fish feed

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    Isolation and characterization of a carotenoid oxygenase gene from Chlorella zofingiensis (Chlorophyta)

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    The green alga Chlorella zofingiensis produces large amounts of the valuable ketocarotenoid astaxanthin under dark, heterotrophic growth conditions, making it potentially employable for commercial production of astaxanthin as feed additives, colorants, and health products. Here, we report the identification and characterization of a β-carotene oxygenase (CRTO) gene that is directly involved in the biosynthesis of ketocarotenoids in C. zofingiensis. The open reading frame of the crtO gene, which is interrupted by three introns of 243, 318, and 351 bp, respectively, encodes a polypeptide of 312 amino acid residues. Only one crtO gene was detected in the genome of C. zofingiensis. Furthermore, the expression of the crtO gene was transiently up-regulated upon glucose treatment. Functional complementation in Escherichia coli showed that the coding protein of the crtO gene not only exhibits normal CRTO activity by converting β-carotene to canthaxanthin via echinenone, but also displays a high enzymatic activity of converting zeaxanthin to astaxanthin via adonixanthin. Based on the bifunctional CRTO, a predicted pathway for astaxanthin biosynthesis in C. zofingiensis is described, and the CRTO is termed as carotenoid 4,4′-β-ionone ring oxygenase. © Springer-Verlag 2005.postprin

    Fossil carotenoids and paleolimnology of meromictic Mahoney Lake, British Columbia, Canada

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    Vertical distribution of fossil carotenoids in a sediment core from meromictic Mahoney Lake was studied. Besides okenone and demethylated okenone, lutein and zeaxanthin and-carotene isomers were identified. No carotenoids typical for purple nonsulfur or green sulfur bacteria were detected. The ratio of zeaxanthin to lutein (above 1:1 in all samples) indicates a dominance of cyanobacteria over green algae in the phytoplankton assemblages of the past. Okenone, which is found exclusively in Chromatiaceae, was the dominating carotenoid in all sediment zones. The oldest sediment layers containing okenone were deposited 11 000 years ago. Between 9000 and 7000 and since 3000 years b.p., Chromatiaceae reached a considerable biomass in the lake. Vertical changes in okenone concentration were not related to changes of paleotemperatures. In contrast, okenone concentrations decreased during periods of volcanic ash input. During most of the lake history, however, mean okenone concentrations were positively correlated with sedimentation rates. This indicates that vertical changes of okenone concentration in the sediment reflect past changes of purple sulfur bacterial biomass in the lake. According to these results, the past limnology of Mahoney Lake resembled that of the present with a sulfide-containing monimolimnion and a well-developed population of okenone-bearing purple sulfur bacteria

    Behavioral and Electrophysiological Effects of Transcranial Direct Current Stimulation of the Parietal Cortex in a Visuo-Spatial Working Memory Task

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    Impairments of working memory (WM) performance are frequent concomitant symptoms in several psychiatric and neurologic diseases. Despite the great advance in treating the reduced WM abilities in patients suffering from, e.g., Parkinson’s and Alzheimer’s disease by means of transcranial direct current stimulation (tDCS), the exact neurophysiological underpinning subserving these therapeutic tDCS-effects are still unknown. In the present study we investigated the impact of tDCS on performance in a visuo-spatial WM task and its underlying neural activity. In three experimental sessions, participants performed a delayed matching-to-sample WM task after sham, anodal, and cathodal tDCS over the right parietal cortex. The results showed that tDCS modulated WM performance and its underlying electrophysiological brain activity in a polarity-specific way. Parietal tDCS altered event-related potentials and oscillatory power in the alpha band at posterior electrode sites. The present study demonstrates that posterior tDCS can alter visuo-spatial WM performance by modulating the underlying neural activity. This result can be considered an important step toward a better understanding of the mechanisms involved in tDCS-induced modulations of cognitive processing. This is of particular importance for the application of electrical brain stimulation as a therapeutic treatment of neuropsychiatric deficits in clinical populations
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