Международный опыт восстановления объектов окружающей среды и его применение при производстве судебно-экологических экспертиз

The paper examines the issues of developing relevant and adequate methods to address the practical challenges of supporting the justice system with objective scientific evidence. Special emphasis is placed on the problem of ambiguity of the concept of «original» state of the environment prior to the investigated event and the choice of practical approaches to rehabilitation of damaged ecosystems.Рассмотрены вопросы разработки актуальных и адекватных методов, направленных на решение практических задач обеспечения судебной системы объективными результатами экспертных исследований. Особое внимание уделено проблеме неопределенности понятия «исходное» состояние объектов окружающей среды до расследуемого события и выбору путей восстановления объектов окружающей среды

Purinergic modulation of microglial cell activation

Microglial cells are resident macrophages in the brain and their activation is an important part of the brain immune response and the pathology of the major CNS diseases. Microglial activation is triggered by pathological signals and is characterized by morphological changes, proliferation, phagocytosis and the secretion of various cytokines and inflammatory mediators, which could be both destructive and protective for the nervous tissue. Purines are one of the most important mediators which regulate different aspects of microglial function. They could be released to the extracellular space from neurons, astrocytes and from the microglia itself, upon physiological neuronal activity and in response to pathological stimuli and cellular damage. Microglial activation is regulated by various subtypes of nucleotide (P2X, P2Y) and adenosine (A1, A2A and A3) receptors, which control ionic conductances, membrane potential, gene transcription, the production of inflammatory mediators and cell survival. Among them, the role of P2X7 receptors is especially well delineated, but P2X4, various P2Y, A1, A2A and A3 receptors also powerfully participate in the microglial response. The pathological role of microglial purine receptors has also been demonstrated in disease models; e.g., in ischemia, sclerosis multiplex and neuropathic pain. Due to their upregulation and selective activation under pathological conditions, they provide new avenues in the treatment of neurodegenerative and neuroinflammatory illnesses

Impact of Treadmill Running and Sex on Hippocampal Neurogenesis in the Mouse Model of Amyotrophic Lateral Sclerosis

Hippocampal neurogenesis in the subgranular zone (SGZ) of dentate gyrus (DG) occurs throughout life and is regulated by pathological and physiological processes. The role of oxidative stress in hippocampal neurogenesis and its response to exercise or neurodegenerative diseases remains controversial. The present study was designed to investigate the impact of oxidative stress, treadmill exercise and sex on hippocampal neurogenesis in a murine model of heightened oxidative stress (G93A mice). G93A and wild type (WT) mice were randomized to a treadmill running (EX) or a sedentary (SED) group for 1 or 4 wk. Immunohistochemistry was used to detect bromodeoxyuridine (BrdU) labeled proliferating cells, surviving cells, and their phenotype, as well as for determination of oxidative stress (3-NT; 8-OHdG). BDNF and IGF1 mRNA expression was assessed by in situ hybridization. Results showed that: (1) G93A-SED mice had greater hippocampal neurogenesis, BDNF mRNA, and 3-NT, as compared to WT-SED mice. (2) Treadmill running promoted hippocampal neurogenesis and BDNF mRNA content and lowered DNA oxidative damage (8-OHdG) in WT mice. (3) Male G93A mice showed significantly higher cell proliferation but a lower level of survival vs. female G93A mice. We conclude that G93A mice show higher hippocampal neurogenesis, in association with higher BDNF expression, yet running did not further enhance these phenomena in G93A mice, probably due to a ‘ceiling effect’ of an already heightened basal levels of hippocampal neurogenesis and BDNF expression

Land Degradation Neutrality — the modern approach for land management and policy-making

The land reform affected all the countries of the post-Soviet space and in the number of cases led to land degradation, as indicated in scientific literature and in the state reports. The paper presents the results of applying the modern international methodology for Land Degradation Neutrality (LDN) assessment for the Russian Federation. The tools of this methodology makes it possible to trace, on a retrospective basis, the dynamics of land degradation using following proxy indicators: land cover, land productivity and soil organic carbon stocks. The relevant data for 2000-2015 are provided in the paper. It is shown that, despite the convenience and relative simplicity of the international methodology, as well as the possibility for comparative analysis of the state of land for the entire territory of the country according to a unified methodology, nevertheless it can not be used as alternative methods through direct substitutions of the global LDN indicators to national counterparts. The main reasons for the contradictions revealed are the variety of socio-economic and socio-economic conditions, as well as the existing multi-level state system for land monitoring and assessment, which is traditional and conservative. To harmonizing national and global data it is proposed to enhance the national land monitoring system with global indicators for using them in the national land assessment system to monitor and verify the accuracy of statistical data obtained by traditional methods. In this case, global LDN indicators can be used in state and sectoral statistics to analyze general trends and stimulate the LDN targeting at the national and local level. The method for LDN calculation allows comparing poorly compared land categories by degradation indicators, although it will require substantial refinement for use in the Russian Federation

Realising the Carbon Benefits of Sustainable Land Management Practices: Guidelines for Estimation of Soil Organic Carbon in the Context of Land Degradation Neutrality Planning and Monitoring

Land degradation is one of the threats to human and natural systems. Fortunately, over the past few decades awareness of this challenge has grown, and 122 countries have committed to setting land degradation neutrality (LDN) targets, of which 84 have officially validated their targets, and 51 have put their targets into legislation. In this concept, LDN is achieved if new degradation is balanced by reversal of degradation elsewhere in the same land type by restoration or rehabilitation. The primary instrument for achieving LDN is through the implementation of sustainable land management (SLM) practices. Because of its multifunctional roles and its sensitivity to land management soil organic carbon (SOC) was selected as one of three indicators for LDN. Compared with the other global LDN indicators, that is, land cover change and land productivity dynamics (LPD) (measured as net primary productivity), SOC is challenging to manage and monitor at large scales. Moreover, SOC density in soils can vary greatly, even on the scale of meters, and fluctuates over time, for example between seasons. Comparative evaluation of SOC change between different SLM options (e.g. for land planning), tracking SOC dynamics through time (i.e. SOC monitoring) and effectively mapping SOC changes at large scales (e.g. for verifying LDN achievement) requires the combination of rigorous soil sampling schemes and the use of software tools/biophysical models for SOC assessment. To provide practical guidance to support the deployment of SLM interventions to maintain or enhance SOC stocks, for LDN and for other objectives such as landbased climate change adaption and/or mitigation a series of decision trees was developed, based on the latest available knowledge. This report reviews and compares available tools and models for SOC estimation. It presents practical guidance for land managers and puts forward policy-oriented proposals. Guidance for land managers emphasizes the selection of SLM practices to maintain or enhance soil organic carbon and achieve LDN. It addresses the choice of SLM practices suited to the local socio-economic and biophysical context; methods for measurement and monitoring of SOC; and the use of tools/ models for SOC assessment to estimate SOC and map SOC, and how to choose an appropriate tool/model according to the purpose. Policy-oriented options include to (i) share the guidance for land managers at the appropriate level; (ii) monitor SOC change as an indicator of SLM intervention to support assessment of LDN achievement in 2030; (iii) apply gender-responsive actions addressing gender-based differences and promote gender equality and women’s empowerment; (iv) design a framework for LDN Planning and means to support it