Design of a novel flow-and-shoot microbeam

Presented here is a novel microbeam technology—the Flow-And-ShooT (FAST) microbeam—under development at RARAF. In this system, cells undergo controlled fluidic transport along a microfluidic channel intersecting the microbeam path. They are imaged and tracked in real-time, using a high-speed camera and dynamically targeted, using a magnetic Point and Shoot system. With the proposed FAST system, RARAF expects to reach a throughput of 100 000 cells per hour, which will allow increasing the throughput of experiments by at least one order of magnitude. The implementation of FAST will also allow the irradiation of non-adherent cells (e.g. lymphocytes), which is of great interest to many of the RARAF users. This study presents the design of a FAST microbeam and results of first tests of imaging and tracking as well as a discussion of the achievable throughput

Design and numerical assessment of a rapid-construction corrugated steel-concrete-steel protective structure

A protective structure should be sufficiently resilient to protect its occupants from the harmful effects of an impact or explosion. In many instances, protective structures are also required to be assembled quickly, and be cost-effective. Steel-concrete-steel (SCS) sandwich structures combine the benefits of steel; ductility and anti-scabbing, and concrete; energy absorption and rigidity. Despite these favourable characteristics, the performance of profiled-plate steel-concrete-steel structures under blast and impact loads has yet to be studied in detail. This article presents the results from a numerical study investigating the efficacy of a newly proposed profiled-plate arched steel-concrete-steel structure under the loading from an extremely near-field high explosive detonation. It is observed that as arch thickness (concrete infill depth) increases, a greater proportion of energy is absorbed through concrete crushing and a larger concrete mass is mobilised. It is shown that a 240 mm arch thickness is adequate to resist the blast load from a 5.76 kg TNT charge, therefore proving the suitability of the proposed protective structure

Angle of Incidence Effects on Far-Field Positive and Negative Phase Blast Parameters

The blast overpressure acting on a rigid target is known to vary between the normally reflected overpressure and the incident overpressure as a function of the angle between the target and the direction of travel of the blast wave. Literature guidance for determining the exact effects of angle of incidence are unclear, particularly when considering the negative phase. This paper presents the results from a series of well controlled experiments where pressure transducers are used to record the pressure-time history acting on the face of a large, rigid target at various angles of incidence for varying sizes of hemispherical PE4 charge and stand-off distances. The test data demonstrated remarkable repeatability, and excellent agreement with semi-empirical predictions for normally reflected overpressures. The oblique results show that peak overpressure, impulse and duration are highly dependent on angle of incidence for the positive phase, and are invariant of angle of incidence for the negative phase

Observations from Preliminary Experiments on Spatial and Temporal Pressure Measurements from Near-Field Free Air Explosions

It is self-evident that a crucial step in analysing the performance of protective structures is to be able to accurately quantify the blast load arising from a high explosive detonation. For structures located near to the source of a high explosive detonation, the resulting pressure is extremely high in magnitude and highly non-uniform over the face of the target. There exists very little direct measurement of blast parameters in the nearfield, mainly attributed to the lack of instrumentation sufficiently robust to survive extreme loading events yet sensitive enough to capture salient features of the blast. Instead literature guidance is informed largely by early numerical analyses and parametric studies. Furthermore, the lack of an accurate, reliable data set has prevented subsequent numerical analyses from being validated against experimental trials. This paper presents an experimental methodology that has been developed in part to enable such experimental data to be gathered. The experimental apparatus comprises an array of Hopkinson pressure bars, fitted through holes in a target, with the loaded faces of the bars flush with the target face. Thus, the bars are exposed to the normally or obliquely reflected shocks from the impingement of the blast wave with the target. Pressure-time recordings are presented along with associated Arbitary-Langrangian-Eulerian modelling using the LS-DYNA explicit numerical code. Experimental results are corrected for the effects of dispersion of the propagating waves in the pressure bars, enabling accurate characterisation of the peak pressures and impulses from these loadings. The combined results are used to make comments on the mechanism of the pressure load for very near-field blast events

A Numerical Investigation of Blast Loading and Clearing on Small Targets

When a blast wave strikes a finite target, diffraction of the blast wave around the free edge causes a rarefaction clearing wave to propagate along the loaded face and relieve the pressure acting at any point it passes over. For small targets, the time taken for this clearing wave to traverse the loaded face will be small in relation to the duration of loading. Previous studies have not shown what happens in the late-time stages of clearing relief, nor the mechanism by which the cleared reflected pressure decays to approach the incident pressure. Current design guidance assumes a series of interacting clearing waves propagate over the target face - this assumption is tested in this article by using numerical analysis to evaluate the blast pressure acting on small targets subjected to blast loads. It is shown that repeat propagations of the rarefaction waves do not occur and new model is proposed, based on an over-expanded region of air in front of the loaded face of the target