3 research outputs found
Development of the Acellular Dermal Matrix and Experimental Substantiation of Its Use in the Anterior Abdominal Wall hernia Repair
Objective:Β To develop a biological implant that is an acellular dermal matrix (ADM), evaluate its use as a support material in tensionfree hernioplasty for ventral hernia, and compare it to that of the commercially available implant PermacolTM.Β Β Materials and methods:Β ADM was derived from the porcine dermis (Landrace breed) decellularized using detergents and enzymes. The quality of devitalization was assessed in vitro. We performed sublay hernioplasty in 4-month-old Landrace pigs using ADM (experimental group) and PermacolTMΒ (control group). The specimens were explanted on day 120 of the experiment for histological and immunohistochemical examination.Β Β Results:Β All cellular elements were removed by the detergent-enzymatic treatment of the dermis; the native architecture of the dermis was slightly disrupted. The specimens of the commercially available chemically cross-linked biomaterial PermacolTMΒ had better mechanical properties than ADM specimens; however, there were no significant differences in terms of cytotoxicity. The state of the tissues after the explantation (number of fibroblasts and endothelial cells) showed no differences in the result of using ADM and PermacolTM. In 120 days, the materials integrated into the tissues without the formation of adhesions or inflammation.Β Β Conclusions:Β Our findings show that ADM does not have cytotoxic properties, has adequate biomechanical parameters to effectively reinforce supporting soft tissues, does not cause an inflammatory response during implantation, and integrates fully into tissues. Our study demonstrates the effectiveness and utility of the developed ADM in surgical treatment of anterior abdominal wall defects
ΠΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ Π΄Π»Ρ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π»Π΅ΡΠ΅Π½ΠΈΡ ΠΏΠΎΠ²Π΅ΡΡ Π½ΠΎΡΡΠ½ΡΡ ΡΠ°Π½
Aim. To study the efficacy of dermal hydrogel application in the experimental treatment of superficial scarified wounds in rats.Materials and methods. The hydrogel was obtained from porcine dermis by alkaline hydrolysis. The DNA concentration was determined using the Nano Drop ND-1000 spectrophotometer. The study included 30 male Sphinx rats. Scarified wounds were created on the rat skin, then the rats were divided into two groups: group 1 β rats without treatment, or control group (n = 15), group 2 β rats with wound treatment with the dermal hydrogel for 5 days, or experimental group (n = 15). On day 3, 7, and 14 of the experiment, we explanted skin samples from the wound area and performed routine H&E staining.Results. On day 3 of the experiment, moderate inflammation, edema, and collagen fiber disorganization were revealed in the experimental group, and pronounced inflammation with purulent exudate was found in the control group. On day 7 of the experiment, inflammation and foci of stratified epithelium were detected in the control group. The histologic analysis of the skin samples from the experimental group showed pronounced plethora of the vessels, necrotic changes of the dermis, and edema. The total thickness of the epidermis and the thickness of its stratum corneum were greater than in the control group samples. On day 14, the differences between the groups were minimal and the epidermis was thickened in the experimental group animals.Conclusion. The study examined the effects of the dermal hydrogel on scarified wounds in rats. We found faster skin regeneration (by 1.5β2 days) in the experimental group compared to the controls. Besides, the rats of the experimental group were characterized by an increase in the number of fibroblasts in the dermis and thickened epidermis in the affected area.Π¦Π΅Π»Ρ β ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΡΡΡΠ΅ΠΊΡΠΈΠ²Π½ΠΎΡΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΡΠΈ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½ΠΎΠΌ Π»Π΅ΡΠ΅Π½ΠΈΠΈ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΡΡ
ΡΠΊΠ°ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠ°Π½ Ρ ΠΊΡΡΡ.ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΈΠ· ΡΠ²ΠΈΠ½ΠΎΠΉ Π΄Π΅ΡΠΌΡ Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠΈΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Ρ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ ΡΠ΅Π»ΠΎΡΠ½ΠΎΠ³ΠΎ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°. Π ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΡΡ
ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ ΠΠΠ Ρ ΠΏΠΎΠΌΠΎΡΡΡ ΡΠΏΠ΅ΠΊΡΡΠΎΡΠΎΡΠΎΠΌΠ΅ΡΡΠ° Nano Drop ND-1000. ΠΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ Π½Π° 30 ΡΠ°ΠΌΡΠ°Ρ
ΠΊΡΡΡ ΠΏΠΎΡΠΎΠ΄Ρ ΡΡΠΈΠ½ΠΊΡ. ΠΡΡΡΠ°ΠΌ Π½Π°Π½ΠΎΡΠΈΠ»ΠΈ ΡΠΊΠ°ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΠ΅ ΡΠ°Π½Ρ, Π·Π°ΡΠ΅ΠΌ ΠΆΠΈΠ²ΠΎΡΠ½ΡΠ΅ Π±ΡΠ»ΠΈ ΡΠ°Π·Π΄Π΅Π»Π΅Π½Ρ Π½Π° Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ: Π³ΡΡΠΏΠΏΠ° 1 β Π±Π΅Π· Π»Π΅ΡΠ΅Π½ΠΈΡ, ΠΈΠ»ΠΈ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½Π°Ρ Π³ΡΡΠΏΠΏΠ° (n = 15), Π³ΡΡΠΏΠΏΠ° 2 β Π»Π΅ΡΠ΅Π½ΠΈΠ΅ ΡΠ°Π½Ρ Π΄Π΅ΡΠΌΠ°Π»ΡΠ½ΡΠΌ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Π΅ΠΌ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 5 ΡΡΡ (n = 15). ΠΠ° 3-ΠΈ, 7-Π΅ ΠΈ 14-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ»Π°Π½ΡΠΈΡΠΎΠ²Π°Π»ΠΈΡΡ ΠΎΠ±ΡΠ°Π·ΡΡ ΠΊΠΎΠΆΠΈ ΠΈΠ· ΠΎΠ±Π»Π°ΡΡΠΈ ΡΠ°Π½Ρ, ΠΊΠΎΡΠΎΡΡΠ΅ ΠΏΠΎΠ΄Π²Π΅ΡΠ³Π°Π»ΠΈΡΡ Π³ΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΠ° 3-ΠΈ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΊΠΎΠΆΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ 2 ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΎΡΡ ΡΠΌΠ΅ΡΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Ρ ΠΏΠΎΠ²Π΅ΡΡ
Π½ΠΎΡΡΠ½ΡΠΌ ΠΎΡΠ΅ΠΊΠΎΠΌ ΠΈ Π΄ΠΈΡΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°ΡΠΈΠ΅ΠΉ ΠΊΠΎΠ»Π»Π°Π³Π΅Π½ΠΎΠ²ΡΡ
Π²ΠΎΠ»ΠΎΠΊΠΎΠ½, Π° ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ β Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅ Ρ Π³Π½ΠΎΠΉΠ½ΡΠΌ ΡΠΊΡΡΡΠ΄Π°ΡΠΎΠΌ. ΠΠ° 7-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° Ρ ΠΊΡΡΡ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ Π½Π°Π±Π»ΡΠ΄Π°Π»ΠΈ Π²ΠΎΡΠΏΠ°Π»Π΅Π½ΠΈΠ΅, ΠΎΠ΄Π½Π°ΠΊΠΎ ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΠΎΡΠ°Π³ΠΈ ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΠΌΠ½ΠΎΠ³ΠΎΡΠ»ΠΎΠΉΠ½ΠΎΠ³ΠΎ ΡΠΏΠΈΡΠ΅Π»ΠΈΡ. ΠΠΈΡΡΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΠΉ Π°Π½Π°Π»ΠΈΠ· ΠΊΠΎΠΆΠΈ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ 2 ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π» Π±ΠΎΠ»Π΅Π΅ Π²ΡΡΠ°ΠΆΠ΅Π½Π½ΠΎΠ΅ ΠΏΠΎΠ»Π½ΠΎΠΊΡΠΎΠ²ΠΈΠ΅ ΡΠΎΡΡΠ΄ΠΎΠ², Π½Π΅ΠΊΡΠΎΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΡ Π΄Π΅ΡΠΌΡ ΠΈ Π΅Π΅ ΠΎΡΠ΅ΠΊ. ΠΠ±ΡΠ°Ρ ΡΠΎΠ»ΡΠΈΠ½Π° ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ° ΠΈ ΡΠΎΠ»ΡΠΈΠ½Π° Π΅Π³ΠΎ ΡΠΎΠ³ΠΎΠ²ΠΎΠ³ΠΎ ΡΠ»ΠΎΡ Π±ΡΠ»Π° Π±ΠΎΠ»ΡΡΠ΅, ΡΠ΅ΠΌ Π² ΠΎΠ±ΡΠ°Π·ΡΠ°Ρ
ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ. ΠΠ° 14-Π΅ ΡΡΡ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ° ΡΠ°Π·Π»ΠΈΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΈΠ·ΡΡΠ°Π΅ΠΌΡΠΌΠΈ Π³ΡΡΠΏΠΏΠ°ΠΌΠΈ Π±ΡΠ»ΠΈ ΠΌΠΈΠ½ΠΈΠΌΠ°Π»ΡΠ½Ρ, ΠΎΡΠΌΠ΅ΡΠ°Π»ΠΈ ΡΡΠΎΠ»ΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ° Ρ ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΡ 2 ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠΎΠΉ.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. Π ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π½ΠΎΠΌ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΠΏΡΠΎΠ΄Π΅ΠΌΠΎΠ½ΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠΈ Π³ΠΈΠ΄ΡΠΎΠ³Π΅Π»Ρ Π½Π° ΠΎΡΠ½ΠΎΠ²Π΅ Π΄Π΅ΡΠΌΡ ΡΠ²ΠΈΠ½ΡΠΈ Π΄Π»Ρ Π»Π΅ΡΠ΅Π½ΠΈΡ ΡΠΊΠ°ΡΠΈΡΠΈΡΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
ΡΠ°Π½ ΠΊΡΡΡ ΠΏΠΎΠ»Π½ΠΎΠ΅ Π²ΠΎΡΡΡΠ°Π½ΠΎΠ²Π»Π΅Π½ΠΈΠ΅ ΠΊΠΎΠΆΠΈ Π² ΠΏΠΎΡΠ°ΠΆΠ΅Π½Π½ΠΎΠΉ ΠΎΠ±Π»Π°ΡΡΠΈ Π½Π°ΡΡΡΠΏΠ°Π»ΠΎ Π½Π° 1,5β2 ΡΡΡ Π±ΡΡΡΡΠ΅Π΅, ΡΠ΅ΠΌ Π² ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΠ΅. ΠΠΎΠΌΠΈΠΌΠΎ ΡΡΠΎΠ³ΠΎ Π±ΡΠ»ΠΎ Π·Π°ΡΠ΅Π³ΠΈΡΡΡΠΈΡΠΎΠ²Π°Π½ΠΎ ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ»ΠΈΡΠ΅ΡΡΠ²Π° ΡΠΈΠ±ΡΠΎΠ±Π»Π°ΡΡΠΎΠ² Π² Π΄Π΅ΡΠΌΠ΅ ΠΈ ΡΡΠΎΠ»ΡΠ΅Π½ΠΈΠ΅ ΡΠΏΠΈΠ΄Π΅ΡΠΌΠΈΡΠ° ΠΎΡΠ½ΠΎΡΠΈΡΠ΅Π»ΡΠ½ΠΎ Π°Π½Π°Π»ΠΎΠ³ΠΈΡΠ½ΠΎΠ³ΠΎ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Ρ Ρ ΠΊΡΡΡ ΠΊΠΎΠ½ΡΡΠΎΠ»ΡΠ½ΠΎΠΉ Π³ΡΡΠΏΠΏΡ