6,337 research outputs found

    Xanthomonas campestris pv. campestris race 1 is the main causal agent of black rot of Brassicas in Southern Mozambique

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    Severe outbreaks of bacterial black rot caused by Xanthomonas campestris pv. campestris (Xcc) were observed in Brassica production fields of Southern Mozambique. The causal agent of the disease in the Mahotas and Chòkwé districts was identified and characterised. In total, 83 Xanthomonas-like strains were isolated from seed samples and leaves of cabbage and tronchuda cole with typical symptoms of the disease. Forty-six out of the 83 strains were found to be putative Xcc in at least one of the tests used: Classical biochemical assays, enzyme-linked immunosorbent assay (ELISA) with monoclonal antibodies, Biolog identification system, polymerase chain reaction (PCR) with specific primers and pathogenicity tests. The ELISA tests were positive for 43 strains. Biolog identified 43 strains as Xanthomonas, but only 32 as Xcc. PCR tests with primers targeting a fragment of the hrpF gene were positive for all 46 strains tested. Three strains were not pathogenic or weakly pathogenic and all other strains caused typical black rot symptoms in brassicas. Race type differentiation tests revealed the Xcc strains from Mozambique as members of race 1. The prevalence of this pathogenic race of the Xcc pathogen in Mozambique should be considered when black rot resistant cultivars are evaluated or introduced into the production regions of this country

    \u3ci\u3eRagwort, Ragwort\u3c/i\u3e

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    Into every life, providence scatters a handful of extraordinary hours charged with creating its unique design— the before and after hours that emboss the weave

    Low-loss criterion and effective area considerations for photonic crystal fibers

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    We study the class of endlessly single-mode all-silica photonic crystal fibers with a triangular air-hole cladding. We consider the sensibility to longitudinal nonuniformities and the consequences and limitations for realizing low-loss large-mode area photonic crystal fibers. We also discuss the dominating scattering mechanism and experimentally we confirm that both macro and micro-bending can be the limiting factor.Comment: Accepted for Journal of Optics A - Pure and Applied Optic

    Neuronal Inhibition under the Spotlight

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    In this issue of Neuron, Lin et al. (2015) report an optical method to precisely manipulate the activity of GABAA receptors by designing a mutant receptor that binds photosensitive compounds. This allows for studying GABAA receptors in situ and represents a valuable tool to investigate how inhibition affects brain physiology

    Imposing high-symmetry and tuneable geometry on lanthanide centres with chelating Pt and Pd metalloligands

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    Exploitation of HSAB preferences allows for high-yield, one-pot syntheses of lanthanide complexes chelated by two Pd or Pt metalloligands, [MII(SAc)4]2− (SAc− = thioacetate, M = Pd, Pt). The resulting complexes with 8 oxygen donors surrounding the lanthanides can be isolated in crystallographically tetragonal environments as either [NEt4]+ (space group: P4/mcc) or [PPh4]+ (space group: P4/n) salts. In the case of M = Pt, the complete series of lanthanide complexes has been structurally characterized as the [NEt4]+ salts (except for Ln = Pm), while the [PPh4]+ salts have been structurally characterized for Ln = Gd–Er, Y. For M = Pd, selected lanthanide complexes have been structurally characterized as both salts. The only significant structural difference between salts of the two counter ions is the resulting twist angle connecting tetragonal prismatic and tetragonal anti-prismatic configurations, with the [PPh4]+ salts approaching ideal D4d symmetry very closely (φ = 44.52–44.61°) while the [NEt4]+ salts exhibit intermediate twist angles in the interval φ = 17.28–27.41°, the twist increasing as the complete 4f series is traversed. Static magnetic properties for the latter half of the lanthanide series are found to agree well in the high temperature limit with the expected Curie behavior. Perpendicular and parallel mode EPR spectroscopy on randomly oriented powder samples and single crystals of the Gd complexes with respectively Pd- and Pt-based metalloligands demonstrate the nature of the platinum metal to strongly affect the spectra. Consistent parametrization of all of the EPR spectra reveals the main difference to stem from a large difference in the magnitude of the leading axial term, B02, this being almost four times larger for the Pt-based complexes as compared to the Pd analogues, indicating a direct Pt(5dz2)–Ln interaction and an arguable coordination number of 10 rather than 8. The parametrization of the EPR spectra also confirms that off-diagonal operators are associated with non-zero parameters for the [NEt4]+ salts, while only contributing minimally for the [PPh4]+ salts in which lanthanide coordination approximates D4d point group symmetry closely.LHD acknowledges support from NSF-CCT EMT 08-517. (08-517 - NSF-CCT EMT

    Equilibrium Labor Turnover, Firm Growth, and Unemployment

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    This paper considers equilibrium quit turnover in a frictional labor market with costly hiring by firms, where large firms employ many workers and face both aggregate and firm specific productivity shocks. There is exogenous firm turnover as new (small) startups enter the market over time, while some existing firms fail and exit. Individual firm growth rates are disperse and evolve stochastically. The paper highlights how dynamic monopsony, where firms trade off lower wages against higher (endogenous) employee quit rates, yields excessive job-to-job quits. Such quits directly crowd out the reemployment prospects of the unemployed. With finite firm productivity states, stochastic equilibrium is fully tractable and can be computed using standard numerical techniques

    Ballistic reflection at a side-gate in a superconductor-semiconductor-superconductor structure

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    We have fabricated a sub-micron-sized structure consisting of an InAs-based 2DEG, two narrow Nb leads and a gate, where the indirect ballistic transport between the non-oppositely superconducting contacts can be controlled by the voltage applied to the gate. This new kind of tuneable junction can be used for applications and allows several fundamental questions related to the transport mechanism to be studied. First results of experiments carried out in this respect are presented.Comment: 6 pages, 4 eps-figure
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