305 research outputs found
Laparoscopic removal of mullerian duct remnants in boys
Abstract: Purpose: Mullerian duct remnants (MDRs) are present in a male pseudohermaphroditic form characterized by failure of the mullerian duct to regress due to insufficient production or peripheral action of mullerian inhibiting substance. The MDR can be asymptomatic but it often results in infections, stones and voiding troubles. Furthermore, it may develop into a neoplasm. Therefore, surgery is mandatory for large MDRs and symptomatic patients. Laparoscopic removal is described.
Materials and Methods: Six males were treated from February 1998 to February 2003. Age at surgery was between 3 and 18 years (mean 8.6). All patients showed severe hypospadias and 2 had mixed gonadal dysgenesis with ambiguous genitalia. Three patients presented with urogenital infections and all had a large MDR. Laparoscopic procedures, which were preceded by cystoscopy, were performed using a 10 mm umbilical trocar for the camera and 3, 5 mm trocars for instruments placed in the suprapubic region and iliac fossa bilaterally. The remnants were ligated with endoscopic loops or an endoscopic GIA stapler and cut.
Results: Mean operative time was 2 hours. We noted no complications. In 2 cases there was deferential ectopia and in another of mixed gonadal dysgenesis bilateral gonadectomy was performed because of the risk of degeneration. Feeding started on postoperative day I and the patients were discharged home on day 5. After a followup of 8 months to 4 years all boys were healthy.
Conclusions: Multiple approaches are used in traditional surgery, often leading to complications. Laparoscopy improves the view, decreases surgical risk and operative time, avoids large scars and allows more rapid hospital discharge
Hepatic epidermoid cyst
open6noWe describe the extraordinary finding of a hepatic epidermoid cyst in a 5-year-old patient, treated successfully with laparoscopic deroofing and mucosal stripping. The pathologic examination revealed a cyst with the same features of a true epithelial splenic cyst whose origin is still controversial, even though a coelomic derivation is the most accredited hypothesis. A cyst in this anatomic district with such characteristics has never been described in existent literature.openDi Salvo, N.*; Libri, M.; Gargano, T.; Salfi N; Ruggeri, G.; Lima, M.Di Salvo, N.*; Libri, M.; Gargano, T.; Salfi N; Ruggeri, G.; Lima, M
Amniotic fluid absorption and growth functions in humans: what can we indirectly learn from congenital digestive atresias?
Background: Amniotic fluid (AF) was thought of just as a mechanical cushioning to the foetus. Nowadays, its role
during pregnancy is getting more attention, suggesting hitherto unknown aspects. The aim of the study is to speculate
on AF nutritional functions and its clinical repercussions based on what digestive tract (DT) atresias seem to
suggest.
Methods: A retrospective analysis of the patients admitted to our department for DT atresias between 2000 and
2020 was conducted. Patients’ birth weight (BW), gestational age (GA) at birth and diagnosis were recorded. The following
were excluded from the study: oesophageal atresias (OA) with tracheoesophageal fistula (TOF), colonic and
anal atresias and patients with associated major comorbidities. A control group was made of patients admitted to
our ward in the same period for congenital pulmonary airway malformations (CPAM). To standardize the BW, it was
coupled with birth GA calculating the newborn percentiles. The mean newborn percentiles of OAs, duodenal atresias
(DAs), jejunal atresias (JAs), and ileal atresias (IAs) were independently compared with the control group using Student’s
t-test. Lastly, the significance of the frequencies’ distribution of newborns born small for gestational age (SGA)
between the DT atresias and the control group was evaluated with the χ2 test, and the OR were calculated. A p-value
< 0.05 was considered statistically significant.
Results: A total of 231 patients were eligible for the study: 36 OAs without TOF, mean BW 2488.8 ± 491 g (range
1630–3750 g), mean GA 36.8 ± 2.1 weeks (31–40 weeks), mean newborn percentile 18 ± 22 (1–75); 20 DAs, mean BW
2586.8 ± 577.9 g (1250–3462 g), mean GA 36.2 ± 2.5 weeks (31–40 weeks), mean newborn percentile 31 ± 23 (3–79);
17 JAs, mean BW 2483.5 ± 621.7 g (900–3205 g), mean GA 34.8 ± 2.1 weeks (30–38 weeks), mean newborn percentile
44 ± 28 (4–96); 17 IAs, mean BW 2646.1 ± 769.8 g (1162.0–3888 g), mean GA 35.9 ± 3.2 weeks (30–41 weeks), mean
newborn percentile 44 ± 26 (1–82); and 141 CPAMs with mean BW 3287.4 ± 492.0 g (980–4580 g), mean GA 38.7 ±
1.8 weeks (26–41 weeks), mean newborn percentile 43 ± 26 (1–99). The number of SGA neonates was 18 between
OA patients (50%), 4 between DAs (20%), 1 between JAs (6%), 2 between IAs (12%) and 11 between CPAMs (8%). The
mean percentile of the OAs and DAs was lower than the control group with a p of < .01 and .03 while no statistical significance
was found in the comparison between DAs, JAs, IAs and CPAMs with a p of .06, .86 and .59. The incidence of
SGA in the control group resulted lower than the one in the DT atresias where it becomes higher the more proximal
the atresia is (p < .05). The OR of SGA newborn in the OA group was 11.8, in DA 3.0, in JA 0.7 and in IA 1.6.
Conclusion: AF showed to have a great impact on foetal growth, and its preferred site of absorption seemed to be
the stomach and duodenum. Its nutritional role could have an important predictive value in diagnosing DT atresia
both prenatally and postnatally
Safety profile of the newest antiepileptic drugs: a curated literature review
BACKGROUND: Despite the introduction of new antiepileptic drugs (AEDs), the quality of life and therapeutic response for patients with epilepsy remain unsatisfactory. In addition, whilst several antiepileptic drugs (AEDs) have been approved and consequently marketed in recent years, little is known about their long-term safety and tolerability. Availability of the newest AEDs, characterized by improved pharmacokinetic profiles, has positively impacted the treatment approach for patients with partial seizures in clinical practice. However, the main cause of treatment failure is still poor patient compliance due to the occurrence of adverse drug reactions (ADRs) that lead to treatment withdrawal in about 25% of cases before achieving maximal efficacy, and is associated with increasing health care costs. METHODS: In this Review, we conducted an online database search using Medline, PubMed, Embase, and the Cochrane Online Library to review the available studies highlighting the clinical relevance of side effects, pharmacological interactions, safety and tolerability of the newest AEDs: Brivaracetam (BRV), Cannabidiol (CBD), Eslicarbazepine acetate (ESL), Lacosamide (LCM), and Perampanel (PER). RESULTS: The principal benefit of the newest AEDs, in addition to reduced frequency and seizure severity, is the low number and severity of ADRs reported compared to more historic drugs. CONCLUSION: Early detection of ADRs could lead to an improvement in patients' quality of life, therefore it is important to monitor ADRs and to adequately perform post marketing surveillance in the clinical practice setting
Neutralising antibody activity against SARS-CoV-2 VOCs B.1.617.2 and B.1.351 by BNT162b2 vaccination
Ambroxol for the Treatment of Patients With Parkinson Disease With and Without Glucocerebrosidase Gene Mutations: A Nonrandomized, Noncontrolled Trial
Importance: Mutations of the glucocerebrosidase gene, GBA1 (OMIM 606463), are the most important risk factor for Parkinson disease (PD). In vitro and in vivo studies have reported that ambroxol increases β-glucocerebrosidase (GCase) enzyme activity and reduces α-synuclein levels. These observations support a potential role for ambroxol therapy in modifying a relevant pathogenetic pathway in PD. Objective: To assess safety, tolerability, cerebrospinal fluid (CSF) penetration, and target engagement of ambroxol therapy with GCase in patients with PD with and without GBA1 mutations. / Interventions: An escalating dose of oral ambroxol to 1.26 g per day. Design, Setting, and Participants: This single-center open-label noncontrolled clinical trial was conducted between January 11, 2017, and April 25, 2018, at the Leonard Wolfson Experimental Neuroscience Centre, a dedicated clinical research facility and part of the University College London Queen Square Institute of Neurology in London, United Kingdom. Participants were recruited from established databases at the Royal Free London Hospital and National Hospital for Neurology and Neurosurgery in London. Twenty-four patients with moderate PD were evaluated for eligibility, and 23 entered the study. Of those, 18 patients completed the study; 1 patient was excluded (failed lumbar puncture), and 4 patients withdrew (predominantly lumbar puncture-related complications). All data analyses were performed from November 1 to December 14, 2018. / Main Outcomes and Measures: Primary outcomes at 186 days were the detection of ambroxol in the CSF and a change in CSF GCase activity. / Results: Of the 18 participants (15 men [83.3%]; mean [SD] age, 60.2 [9.7] years) who completed the study, 17 (8 with GBA1 mutations and 9 without GBA1 mutations) were included in the primary analysis. Between days 0 and 186, a 156-ng/mL increase in the level of ambroxol in CSF (lower 95% confidence limit, 129 ng/mL; P < .001) was observed. The CSF GCase activity decreased by 19% (0.059 nmol/mL per hour; 95% CI, -0.115 to -0.002; P = .04). The ambroxol therapy was well tolerated, with no serious adverse events. An increase of 50 pg/mL (13%) in the CSF α-synuclein concentration (95% CI, 14-87; P = .01) and an increase of 88 ng/mol (35%) in the CSF GCase protein levels (95% CI, 40-137; P = .002) were observed. Mean (SD) scores on part 3 of the Movement Disorders Society Unified Parkinson Disease Rating Scale decreased (ie, improved) by 6.8 (7.1) points (95% CI, -10.4 to -3.1; P = .001). These changes were observed in patients with and without GBA1 mutations. / Conclusions and Relevance: The study results suggest that ambroxol therapy was safe and well tolerated; CSF penetration and target engagement of ambroxol were achieved, and CSF α-synuclein levels were increased. Placebo-controlled clinical trials are needed to examine whether ambroxol therapy is associated with changes in the natural progression of PD. Trial Registration: ClinicalTrials.gov identifier: NCT02941822; EudraCT identifier: 2015-002571-24
Protocol of a randomized, double-blind, placebo-controlled, parallel-group, multicentre study of the efficacy and safety of nicotinamide in patients with Friedreich ataxia (NICOFA)
Introduction: Currently, no treatment that delays with the progression of Friedreich ataxia is available. In the
majority of patients Friedreich ataxia is caused by homozygous pathological expansion of GAA repeats in the first
intron of the FXN gene. Nicotinamide acts as a histone deacetylase inhibitor. Dose escalation studies have shown,
that short term treatment with dosages of up to 4 g/day increase the expression of FXN mRNA and frataxin protein
up to the levels of asymptomatic heterozygous gene carriers. The long-term effects and the effects on clinical
endpoints, activities of daily living and quality of life are unknown.
Methods: The aim of the NICOFA study is to investigate the efficacy and safety of nicotinamide for the treatment of
Friedreich ataxia over 24 months. An open-label dose adjustment wash-in period with nicotinamide (phase A: weeks 1–4)
to the individually highest tolerated dose of 2–4 g nicotinamide/day will be followed by a 2 (nicotinamide group): 1
(placebo group) randomization (phase B: weeks 5–104). In the nicotinamide group, patients will continue with their
individually highest tolerated dose between 2 and 4 g/d per os once daily and the placebo group patients will be
receiving matching placebo. Safety assessments will consist of monitoring and recording of all adverse events and serious
adverse events, regular monitoring of haematology, blood chemistry and urine values, regular measurement of vital signs
and the performance of physical examinations including cardiological signs. The primary outcome is the change in the
Scale for the Assessment and Rating of Ataxia (SARA) over time as compared with placebo in patients with Friedreich
ataxia based on the linear mixed effect model (LMEM) model. Secondary endpoints are measures of quality of life,
functional motor and cognitive measures, clinician’s and patient’s global impression-change scales as well as the upregulation of the frataxin protein level, safety and survival/death.
Perspective: The NICOFA study represents one of the first attempts to assess the clinical efficacy of an epigenetic
therapeutic intervention for this disease and will provide evidence of possible disease modifying effects of nicotinamide
treatment in patients with Friedreich ataxia
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