9,813 research outputs found
Activation of type II calcium/calmodulin-dependent protein kinase by Ca^(2+)/calmodulin is inhibited by autophosphorylation of threonine within the calmodulin-binding domain
It is now well established that autophosphorylation of a threonine residue located next to each calmodulin-binding domain in the subunits of type II Ca^(2+)/calmodulin-dependent protein kinase causes the kinase to remain active, although at a reduced rate, after Ca^(2+) is removed from the reaction. This autophosphorylated form of the kinase is still sensitive to Ca2+/calmodulin, which is required for a maximum catalytic rate. After removal of Ca^(2+), new sites are autophosphorylated by the partially active kinase. Autophosphorylation of these sites abolishes sensitivity of the kinase to Ca^(2+)/calmodulin (Hashimoto, Y., Schworer, C. M., Colbran, R. J., and Soderling, T. R. (1987) J. Biol. Chem. 262, 8051-8055). We have identified two pairs of homologous residues, Thr^(305) and Ser^(314) in the alpha subunit and Thr^(306) and Ser^(315) in the beta subunit, that are autophosphorylated only after removal of Ca^(2+) from an autophosphorylation reaction. The sites were identified by direct sequencing of labeled tryptic phosphopeptides isolated by reverse-phase high pressure liquid chromatography. Thr^(305-306) is rapidly dephosphorylated by purified protein phosphatases 1 and 2A, whereas Ser^(314-315) is resistant to dephosphorylation. We have shown by selective dephosphorylation that the presence of phosphate on Thr^(305-306) blocks sensitivity of the kinase to Ca^(2+)/calmodulin. In contrast, the presence of phosphate on Ser^(314-315) is associated with an increase in the Kact for Ca^(2+)/calmodulin of only about 2-fold, producing a relatively small decrease in sensitivity to Ca^(2+)/calmodulin
New Techniques for Relating Dynamically Close Galaxy Pairs to Merger and Accretion Rates : Application to the SSRS2 Redshift Survey
We introduce two new pair statistics, which relate close galaxy pairs to the
merger and accretion rates. We demonstrate the importance of correcting these
(and other) pair statistics for selection effects related to sample depth and
completeness. In particular, we highlight the severe bias that can result from
the use of a flux-limited survey. The first statistic, denoted N_c, gives the
number of companions per galaxy, within a specified range in absolute
magnitude. N_c is directly related to the galaxy merger rate. The second
statistic, called L_c, gives the total luminosity in companions, per galaxy.
This quantity can be used to investigate the mass accretion rate. Both N_c and
L_c are related to the galaxy correlation function and luminosity function in a
straightforward manner. We outline techniques which account for various
selection effects, and demonstrate the success of this approach using Monte
Carlo simulations. If one assumes that clustering is independent of luminosity
(which is appropriate for reasonable ranges in luminosity), then these
statistics may be applied to flux-limited surveys.
These techniques are applied to a sample of 5426 galaxies in the SSRS2
redshift survey. Using close dynamical pairs, we find N_c(-21<M_B<-18) =
0.0226+/-0.0052 and L_c(-21<M_B<-18) = 0.0216+/-0.0055 10^{10} h^2 L_sun at
z=0.015. These are the first secure estimates of low-z close pair statistics.
If N_c remains fixed with redshift, simple assumptions imply that ~ 6.6% of
present day galaxies with -21<M_B<-18 have undergone mergers since z=1. When
applied to redshift surveys of more distant galaxies, these techniques will
yield the first robust estimates of evolution in the galaxy merger and
accretion rates. [Abridged]Comment: 26 pages (including 10 postscript figures) plus 3 gif figures.
Accepted for publication in ApJ. Paper (including full resolution images)
also available at http://www.astro.utoronto.ca/~patton/ssrs2, along with
associated pair classification experiment (clickable version of Figure 5
Analysis of Borrelia burgdorferi Surface Proteins as Determinants in Establishing Host Cell Interactions
Borrelia burgdorferi infection causes Lyme borreliosis in humans, a condition which can involve a systemic spread of the organism to colonize various tissues and organs. If the infection is left untreated by antimicrobials, it can lead to manifestations including, arthritis, carditis, and/or neurological problems. Identification and characterization of B. burgdorferi outer membrane proteins that facilitate cellular attachment and invasion to establish infection continue to be investigated. In this study, we sought to further define putative cell binding properties of surface-exposed B. burgdorferi proteins by observing whether cellular adherence could be blocked by antibodies. B. burgdorferi mixed separately with monoclonal antibodies (mAbs) against outer surface protein (Osp) A, OspC, decorin-binding protein (Dbp) A, BBA64, and RevA antigens were incubated with human umbilical vein endothelial cells (HUVEC) and human neuroglial cells (H4). B. burgdorferi treated with anti-OspA, -DbpA, and -BBA64 mAbs showed a significant decrease in cellular association compared to controls, whereas B. burgdorferi treated with anti-OspC and anti-RevA showed no reduction in cellular attachment. Additionally, temporal transcriptional analyses revealed upregulated expression of bba64, ospA, and dbpA during coincubation with cells. Together, the data provide evidence that OspA, DbpA, and BBA64 function in host cell adherence and infection mechanisms
Fundamental Magnetic Properties and Structural Implications for Nanocrystalline Fe-Ti-N Thin Films
The magnetization (M) as a function of temperature (T) from 2 to 300 K and
in-plane field (H) up to 1 kOe, room temperature easy and hard direction
in-plane field hysteresis loops for fields between -100 and +100 Oe, and 10 GHz
ferromagnetic resonance (FMR) profiles have been measured for a series of
soft-magnetic nano-crystalline 50 nm thick Fe-Ti-N films made by magnetron
sputtering in an in-plane field. The nominal titanium concentration was 3 at. %
and the nitrogen concentrations (xN) ranged from zero to 12.7 at. %. The
saturation magnetization (Ms) vs. T data and the extracted exchange parameters
as a function of xN are consistent with a lattice expansion due to the addition
of interstitial nitrogen in the body-centered-cubic (bcc) lattice and a
structural transition to body-centered-tetragonal (bct) in the 6-8 at. %
nitrogen range. The hysteresis loop and FMR data show a consistent picture of
the changes in both the uniaxial and cubic anisotropy as a function of xN.
Films with xN > 1.9 at. % show an overall uniaxial anisotropy, with an
anisotropy field parameter Hu that increases with xN. The corresponding
dispersion averaged uniaxial anisotropy energy density parameter = HuMs/2
is a linear function of xN, with a rate of increase of 950 erg/cm3 per at. %
nitrogen. The estimated uniaxial anisotropy energy per nitrogen atom is 30
J/mol, a value consistent with other systems. For xN below 6 at. %, the scaling
of coercive force Hc data with the sixth power of the grain size D indicate a
grain averaged effective cubic anisotropy energy density parameter that is
about an order of magnitude smaller that the nominal K1 values for iron, and
give a quantitative vs. D response that matches predictions for exchange
coupled random grains with cubic anisotropy.Comment: 13 pages, 7 figure
Strongly enhanced photon collection from diamond defect centres under micro-fabricated integrated solid immersion lenses
The efficiency of collecting photons from optically active defect centres in
bulk diamond is greatly reduced by refraction and reflection at the diamond-air
interface. We report on the fabrication and measurement of a geometrical
solution to the problem; integrated solid immersion lenses (SILs) etched
directly into the surface of diamond. An increase of a factor of 10 was
observed in the saturated count-rate from a single negatively charged
nitrogen-vacancy (NV-) within a 5um diameter SIL compared with NV-s under a
planar surface in the same crystal. A factor of 3 reduction in background
emission was also observed due to the reduced excitation volume with a SIL
present. Such a system is potentially scalable and easily adaptable to other
defect centres in bulk diamond.Comment: 5 Pages, 5 figures (4 subfigures) - corrected typ
A Hubble Space Telescope Snapshot Survey of Dynamically Close Galaxy Pairs in the CNOC2 Redshift Survey
We compare the structural properties of two classes of galaxies at
intermediate redshift: those in dynamically close galaxy pairs, and those which
are isolated. Both samples are selected from the CNOC2 Redshift Survey, and
have redshifts in the range 0.1 < z <0.6. Hubble Space Telescope WFPC2 images
were acquired as part of a snapshot survey, and were used to measure bulge
fraction and asymmetry for these galaxies. We find that paired and isolated
galaxies have identical distributions of bulge fractions. Conversely, we find
that paired galaxies are much more likely to be asymmetric (R_T+R_A >= 0.13)
than isolated galaxies. Assuming that half of these pairs are unlikely to be
close enough to merge, we estimate that 40% +/- 11% of merging galaxies are
asymmetric, compared with 9% +/- 3% of isolated galaxies. The difference is
even more striking for strongly asymmetric (R_T+R_A >= 0.16) galaxies: 25% +/-
8% for merging galaxies versus 1% +/- 1% for isolated galaxies. We find that
strongly asymmetric paired galaxies are very blue, with rest-frame B-R colors
close to 0.80, compared with a mean (B-R)_0 of 1.24 for all paired galaxies. In
addition, asymmetric galaxies in pairs have strong [OII]3727 emission lines. We
conclude that close to half of the galaxy pairs in our sample are in the
process of merging, and that most of these mergers are accompanied by triggered
star formation.Comment: Accepted for publication in the Astronomical Journal. 40 pages,
including 15 figures. For full resolution version, please see
http://www.trentu.ca/physics/dpatton/hstpairs
Self-Referential Noise and the Synthesis of Three-Dimensional Space
Generalising results from Godel and Chaitin in mathematics suggests that
self-referential systems contain intrinsic randomness. We argue that this is
relevant to modelling the universe and show how three-dimensional space may
arise from a non-geometric order-disorder model driven by self-referential
noise.Comment: Figure labels correcte
Health Risk Screening and Counselling of Adolescents in Primary Care: a Cluster Randomised Controlled Trial
The research reported in this paper is a project of the Australian Primary Health Care Research Institute which is supported by a grant from the Australian Government Department of Health and Ageing under the Primary Health Care Research Evaluation and Development Strategy
Motion-base simulator study of control of an externally blown flap STOL transport aircraft after failure of an outboard engine during landing approach
A moving-base simulator investigation of the problems of recovery and landing of a STOL aircraft after failure of an outboard engine during final approach was made. The approaches were made at 75 knots along a 6 deg glide slope. The engine was failed at low altitude and the option to go around was not allowed. The aircraft was simulated with each of three control systems, and it had four high-bypass-ratio fan-jet engines exhausting against large triple-slotted wing flaps to produce additional lift. A virtual-image out-the-window television display of a simulated STOL airport was operating during part of the investigation. Also, a simple heads-up flight director display superimposed on the airport landing scene was used by the pilots to make some of the recoveries following an engine failure. The results of the study indicated that the variation in visual cues and/or motion cues had little effect on the outcome of a recovery, but they did have some effect on the pilot's response and control patterns
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