20 research outputs found
Comparative in vitro studies on native and recombinant human cationic trypsins - Cathepsin B is a possible pathological activator of trypsinogen in pancreatitis
Hereditary pancreatitis, an autosomal dominant disease is
believed to
be caused by mutation in the human trypsinogen gene. The role of
mutations has been investigated by in vitro studies using
recombinant
rat and human trypsinogen (TG), In this study we compare the
enzymatic
properties and inhibition by human pancreatic secretory trypsin
inhibitor (hPSTI) of the native, postsynthetically modified and
recombinant cationic trypsin, and found these values practically
identical, We also determined the autolytic stability of
recombinant
wild type (Hu1Asn21) and pancreatitis-associated (Hu1Ile21)
trypsin,
Both forms were equally stable. Similarly, we found no
difference in
the rate of activation of the two zymogens by human cationic and
anionic trypsin. Mesotrypsin did not activate either form. The
rate of
autocatalytic activation of Hu1Asn21 TG and Hu1Ile21 TG was also
identical at pH 8 both in the presence and absence of Ca2+. At
pH 5
Hu1Ile21 TG autoactivated about twice as fast as HulAsn21 TG,
The
presence of physiological amount of hPSTI completely prevented
autoactivation of both zymogens at pH 8 and at pH 5 as well.
Cathepsin
B readily activated both zymogens although Hu1Ile21 TG was
activated
about 2.5-3 times as fast as Hu1Asn21 TG, The presence of hPSTI
did not
prevent the activation of zymogens by cathepsin B, Our results
underlie
the central role of cathepsin B in the development of different
forms
of pancreatitis
A new committee dedicated to understanding and helping hand trauma management in Europe: The FESSH Hand Trauma Committee
Phytoplankton nitrogen demand and the significance of internal and external nitrogen sources in a large shallow lake (Lake Balaton, Hungary)
Since the middle of 1990s the trend of Lake Balaton towards an increasingly trophic status has been reversed, but N2-fixing cyanobacteria are occasionally dominant, endangering water quality in summer. The sources of nitrogen and its uptake by growing phytoplankton were therefore studied. Experiments were carried out on samples collected from the middle of the Eastern (Siófok) and Western (Keszthely) basins between February and October 2001. Ammonium, urea and nitrate uptake and ammonium regeneration were measured in the upper 5-cm layer of sediment using the <sup>15</sup>N-technique. Ammonium was determined by an improved microdiffusion assay. N2 fixation rates were measured by the acetylene-reduction method. Ammonium regeneration rates in the sediment were similar in the two basins. They were relatively low in winter (0.13 and 0.16 μg N cm<sup>−3</sup> day<sup>−1</sup> in the Eastern and Western basin, respectively), increased slowly in the spring (0.38 and 0.45 μg N cm<sup>−3</sup> day<sup>−1</sup>) and peaked in late summer (0.82 and 1.29 μg N cm−3 day−1, respectively). Ammonium uptake was predominant in spring in the Eastern basin and in summer in the Western basin, coincident with the cyanobacterial bloom. The amount of N2 fixed was less than one third of the internal load during summer when external N loading was insignificant. Potentially, the phytoplankton N demand could be supported entirely by the internal N load via ammonium regeneration in the water column and sediment. However, the quantity of N from ammonium regeneration in the upper layer of sediment combined with that from the water column would limit the standing phytoplankton crop in spring in both basins and in late summer in the Western basin, especially when the algal biomass increases suddenly