Plasma levels of HDL particle subpopulations.

<p>Total lipoprotein mass was calculated as the sum of the mass of total protein, CE, FC, PL and TG for each HDL subpopulation isolated from CETP-deficient subjects (n = 9) and from family control subjects (n = 9).</p

Antioxidative activity of HDL particles from CETP-deficient (n = 9) and family control (n = 9) subjects.

<p>Reference LDL (10 mg TC/dl) was incubated at 37°C in PBS in the presence of AAPH (1 mmol/l). Small, dense HDL3b (A), HDL3c (B) or total HDL (C) particles were added to LDL directly before oxidation at 10 mg total mass/dl (A, B) or 40 mg total mass/dl (C). Accumulation of conjugated dienes was continuously measured as the increment in absorbance at 234 nm; two characteristic phases were identified, the lag phase and the propagation phase. To characterise the oxidation kinetics, oxidation rate within the propagation phase and duration of this phase were calculated for each absorbance curve.</p

Biological and clinical characteristics of subjects.

***<p>p<0.001, *p<0.05 vs controls. Numbers in parentheses denote % differences vs. controls.</p

24-hour urinary steroid excretion in male <i>ABCA1</i> and <i>LCAT</i> mutation carriers compared to age-matched female controls.

<p>Data are presented as mean ± SD. P values for student’s t-test.</p

Characteristics of female ATP-binding cassette transporter 1 <i>(ABCA1</i>) and Lecithin-cholesterol acyltransferase (<i>LCAT</i>) mutation carriers and matched female controls.

<p>Values are means ± SD unless otherwise indicated. Triglycerides are median with interquartile range. P for Student’s T-test. Triglycerides were logtransformed prior to T-test.</p>#<p>P for χ² test. No t-test was performed for history of coronary artery disease since referral bias was present. Partial overlap exists between the two control cohorts.</p

Urinary steroid metabolites in female <i>ABCA1</i> and <i>LCAT</i> mutation carriers compared to age-matched female controls.

<p>Data are presented as mean ± SD. P values for student’s t-test.</p

Peak serum cortisol increase after cosyntropin administration in female <i>ABCA1</i> and <i>LCAT</i> mutation carriers versus female controls.

<p>Data are presented as mean ± SD. P values for Student’s T-test.</p

Sphingosine-1-phosphate levels in HDL, HDL2, and HDL3.

<p>Data are expressed as mean±SD.</p

Effects of HDL isolated from carriers of CETP mutations and controls on VCAM-1 expression in TNFα-stimulated HUVEC.

<p>Cells were incubated overnight with HDL, HDL2, or HDL3 isolated from 7 heterozygous carriers of CETP mutations and age-sex matched controls (n = 8), at the concentration of 1.0 mg of protein/ml, before stimulation with TNFα for 8 hours. Results are expressed as percentage of VCAM-1 concentration in conditioned media of untreated TNFα-stimulated cells. Data points for each study participant are shown.</p

GGE analysis of purified HDL2 and HDL3.

<p>HDL fractions isolated from the homozygote, and a representative heterozygote and control were analyzed by GGE.</p