Studies on the mechanism of inhibition of growth of Vibrio cholerae by erythrose

Erythrose strongly inhibits the growth of Vibrio cholerae and Vibrio eltor. The inhibition can be reversed by washing the cells free from erythrose with sterile normal saline. The respiration of V. cholerae cells in the presence of glucose is markedly inhibited by erythrose but the oxygen uptake of cell-free extract under the same conditions is not affected. From the results of experiments on the uptake of [14C] glucose and [α-14C]methylglucoside, it may be concluded that erythrose inhibits the transport of glucose across the cell membrane and thereby inhibits the growth of the organism

Awareness of cord blood banking among pregnant women in semi urban area

Background: Hematopoietic stem cells (HSCs) are multipotent stem cells, derived from bone marrow, peripheral blood and umbilical cord. These HSC are accepted method of treatment for various disorders. Cord blood can be stored either in private or public bank. Awareness about cord blood banking among semi urban and rural population is less compared to urban population. The aim of our study is to evaluate the awareness of cord blood banking among pregnant women in semi urban area and to evaluate their willingness for donating to public blood bank.Methods: This is a cross sectional study done among 365 pregnant women attending antenatal OPD in Obstetrics and Gynecology department of SMVMCH, Puducherry.Results: Forty five percent of pregnant women had knowledge about uses of umbilical cord blood usage during pregnancy. Only 5 % of pregnant women knew about cost of stem cell storage. Less than 1% knew about availability of public cord blood banking. After informing about availability of public cord blood storage 72% of pregnant women were willing to donate to public bank.Conclusions: Knowledge about cord blood banking is lacking among pregnant women. Especially majority of them are unaware of public cord blood banking. Private banking will be useful for those with family members affected with diseases amenable to HSC transplantation. More support from government and private organizations are required, which will help in better functioning of public cord blood bank services

Hemoperitoneum: a diagnostic dilemma. A solid ovarian tumour mimicking ruptured ectopic pregnancy

39 year old sterilized women presented like ruptured ectopic pregnancy with 40 days amenorrhea, pain, and shock Urine Pregnancy Test negative. Culdocentesis was positive. Ultra sonogram confirmed hemoperitoneum and TO mass. Laparotomy revealed left solid ovarian tumour with tumour mass protruding from perforated site and hemoperitoneum. Histological diagnosis was granulosa cell (GC) tumour Stage 1c

Site and species selection criteria for cage culture

Site selection is the most important factor which determines the commercial viability of mariculture systems. Cage culture can be made possible only when the site for cage culture operation is located, designed and operated to provide optimum water quality and to avoid stress conditions. In addition to water and sediment quality of the site some biological and natural distribution information for the species should also be known before a site is selected for cage culture. The selection of fish for cage culture should be based on biological criteria, such as physiological, behavioural characteristics and level of domestication; marketing criteria and environmental criteria, distribution and habitat of sit

Hypercarbons in polyhedral structures

Though carbon is mostly tetravalent and tetracoordinated, there are several examples where the coordination number exceeds four. Structural varieties that exhibit hypercarbons in polyhedral structures such as polyhedral carboranes, sandwich complexes, encapsulated polyhedral structures and novel planar aromatic systems with atoms embedded in the middle are reviewed here. The structural variety anticipated with hypercoordinate carbon among carboranes is large as there are many modes of condensation that could lead to large number of new patterns. The relative stabilities of positional isomers of polyhedral carboranes, sandwich structures, and endohedral carboranes are briefly described. The mno rule accounts for the variety of structural patterns. Wheel-shaped and planar hypercoordinated molecules are recent theoretical developments in this area

Evaluation of complications of abdominal and vaginal hysterectomy

Background: Hysterectomy is one of the most common surgical procedures performed by the gynaecologist.  It can be performed by vaginal and abdominal route. Gynaecologic surgeons worldwide continue to use the abdominal approach for a large majority of hysterectomies that could be performed vaginally despite well-documented evidence that vaginal hysterectomy has better outcome. Aim of our study is to compare vaginal route versus abdominal route of hysterectomy in terms of intra operative and post operative complications.Methods: A retrospective study was done in 229 women who had undergone hysterectomy (176 abdominal hysterectomies and 53 vaginal hysterectomies) from January 2010 to July 2012. Information on the indications, operative procedures, and complications were extracted and analysed.Results: The mean duration of surgery in VH group was 79.6 min and that of TAH group was 99.2 min. The mean blood loss was also more in case of TAH group than that of VH group (215ml vs. 167ml). Bladder injury occurred in 1 case in VH (1.9%) and in 4 cases in TAH (2.3%). Ureter injury occurred in 1(0.6%) case in TAH group. Wound infection developed in 10 cases of TAH group whereas only one case in VH group developed vault infection.Conclusions: Vaginal hysterectomy is associated with quicker recovery, early mobilization, shorter hospitalization, less operative and post operative morbidity when compared to abdominal hysterectomy

E-learning as a tool to impart knowledge of surgical procedures in the undergraduate medical teaching

Background: E-learning is in a budding stage in developing countries. As present-day students are gadget-friendly, introducing a surgical e-learning module may create interest and help in understanding the surgeries easily. This study aimed to determine the effectiveness of E-learning in improving the knowledge component of surgical procedures for undergraduate students and to analyze students’ feedback regarding its usefulness. Methods: This quasi-experimental study was conducted among 67 final-year MBBS students, who had completed 1 week of operation theatre (OT) posting in obstetrics and gynecology. Recording of videos for must-know surgeries in gynecology was done. Video editing and audio description of surgical steps were done. A YouTube channel was created and surgical videos were uploaded. Links to 2 surgical videos and additional teaching materials were uploaded to the college learning management system daily for 2 consecutive weeks. A telegram group was formed for posting questions daily. Pre-test and post-test were conducted and the results were analyzed. Results: The mean marks obtained in the pre-test were 6.567±1.9 and the post-test was 15.22±2.5 (Paired t test p=0.0001). Students mentioned that combining OT posting with an e-learning module helped them to build their knowledge of surgical procedures. Telegram quizzes helped them to increase their understanding of surgeries. 34 students wanted e-learning module to be conducted on other topics also. Conclusions: E-learning is highly effective in improving knowledge component of surgical procedures for undergraduate students. E-learning can be made more effective by adding formative assessment. Combining surgical e-learning module with operation theatre posting can enhance knowledge and understanding

Loose shell syndrome (LSS) of cultured Penaeus monodon - microbiological and histopathological investigations

Investigations were undertaken on loose shell syndrome (LSS) of cultured Penaeus monodon during the period 2009 - 2010. The infected shrimps were collected from culture ponds of east and west Godavari districts in Andhra Pradesh, India and were subjected to microbiological and histopathological studies. Four species of Vibrio were isolated from the diseased shrimps and were identified as V. harveyi, V. alginolyticus, V. metschnikovii and V. fluvialis based on morphological characteristics and biochemical tests. Histopathological studies revealed the presence of occlusion and inclusion bodies of monodon baculovirus (MBV), hepatopancreatic parvo-like virus (HPV), and white spot syndrome virus (WSSV) in hepatopancreatic and gill tissues. All the LSS affected shrimps collected during the present study were found infected with V. harveyi and concurrent infections of other Vibrio species were observed in 40% of the samples. Prevalence of infection with WSSV, MBV and HPV was less compared to Vibrio infections. Granuloma formation was observed in the affected tissues due to bacterial invasions. Multiple viral infections in association with Vibrio sp. were also observed in 2% of LSS affected shrimp

Note on a bumper catch of Threadfin sea catfish in shore seine

For past several years catfish did not form a fishery at Karwar located in the Uttara Kannada District of Karnataka. However, during the routine observations on the fish landings at Aligadda Fish Landing Centre, Karwar, an unusual bumper catch of about 3 tons of cat fish Arius arius was recorded from shore seine (Yendi bale) in the early hours of 28th July 2018. locally known as "Billi Shade", the catch was so heavy that it was difficult to drag the net to the shore and the haul was partially harvested at sea itself. The net operated by around 40 fishers at a depth of less than 8 metres, was 400 m in length

DIRECTIONAL ANTENNA BASED EFFICIENT LOCATION AWARE ROUTING IN MOBILE ADHOC NETWORK

Mobile Adhoc Network (MANET) also called as wireless ad hoc network is a self-organizing, self-configuring infrastructure less network containing a group of mobile nodes communicating wirelessly. As the hosts move often resulting in dynamic topology of the network, routing seeks more attention. Therefore, routing protocol using node’s location information like LAR (location aided routing) has emerged as potential solution. Here, the route discovery is limited to a small region named as request zone in contrast to blind flooding over the entire network. Also it is noticeable that the shape and size of the request zone play a vital role in enhancing the protocol’s performance. After various analyses it was concluded that for higher node density, elliptical shaped request zone performs better than other possible shapes. Further, suitable route must be chosen based on current load status of the network so that successful delivery of packets is ensured. Generally, omni-directional antennas are used for communication between moving motes. The disadvantage of mobile ad hoc networks with omni-directional antenna lies in the limited capacity caused by high interference and low spatial reuse. This paper focuses on obtaining optimal size for request zone in accordance with varying node density. Further, optimal path between source and destination is selected using Dijkstra’s algorithm. Our simulation results show that directional antennas outshines the performance of omni-directional antennas in increasing transmission range of nodes, reducing the number of redundant nodes involving in data communication etc