10 research outputs found
Cytokines and regulation of glucose and lipid metabolism in the obesity
The article presents data of the influence of cytokines of different directions of glucose and lipid metabolism in obesity. A change of the basic paradigm regarding adipose tissue has contributed to a number of recent discoveries. This concerns such basic concepts as healthy and diseased adipocytes, and, as a consequence, changes of their metabolism under the influence of cytokins. Distinguishing the concept of organokines demonstrates that despite the common features of cytokine regulation, each organ has its own specifics features of cytokine regulation, each organ has its own specific an important section of this concept is the idea of the heterogeneity of adipose tissue. Knowledge of the function of adipose tissue localized in different compartments of the body is expanding. There are date about the possibility of transition of one type of adipose tissue to another. A possible mechanism linking adipose tissue inflammation and the formation of insulin resistance (IR) is presented in this paper. The mechanism of IR development is closely connected with to proinflammatory cytokins disordering the insulin signal, accompanied by a decrease of the work of glucose transporters. A decrease of the income of glucose into cells leads to a change of glycolysis level to an increase of the fatty acids oxidation. Cytokins are able to participate in the process of the collaboration of some cells with others, that occurs both during physiological and pathological process
Features of the immune response in children with acute respiratory infections caused by DNA and RNA viruses
The issues of immunopathogenesis of acute respiratory viral infections (ARVI) remain relevant, despite a long history of study. The aim of the study was to investigate the characteristics of the content of some cytokines in the blood serum of children with ARVI caused by DNA and RNA-containing viruses.Β Material and methods.Β We examined 92 children with ARVI at the age from 1 to 15 years, hospitalized in the hospital of the Childrenβs Clinical Hospital No. 6 (Novosibirsk). In order to determine the etiological factor, a study was carried out using the RT-PCR method (test systems AmpliSensORVI-screen-FL, βInterLabServiceβ, Russia) of mucus from the nose and throat for the presence of genetic material of viruses that cause ARVI. Determination of the content of IL-8, IL-17, IFN-Ξ³ in the blood serum in children of all groups was carried out by the method of solid-phase ELISA using commercial test systems of LLC βCytokinβ (Russia).Β Results and discussion.Β 4 groups were formed: group I (nΒ = 20) children with ARVI caused by DNA-containing viruses (group B, C, E adenoviruses, bocaviruses); group II (nΒ = 53) children with ARVI caused by RNA viruses (RS virus, metapneumovirus, parainfluenza viruses 1, 2, 3, 4, rhinovirus and coronoviruses); group III (nΒ = 12) children with ARVI caused by mixed infection; group IV (nΒ = 7) β the genetic material of the pathogen has not been isolated. Concentration of IL-8, IL-17, IFN-Ξ³ was significantly increased in all groups compared to the normative values. Higher values of IFN-Ξ³ and IL-8 were revealed in patients with DNA viruses compared with the group with RNA-containing viruses, although the excess of IFN-Ξ³ was not statistically significant. Differences in the content of IFN-Ξ³, IL-8 and IL-17 were noted for various etiological viral agents, but they were not significant.Β Conclusions.Β The results obtained can serve as confirmation that the functional characteristics of the response of the immune system in children with ARVI are determined not so much by the etiological factor as by its individual state. With ARVI in children, the presence of genetic material from more than one virus, apparently, is not a significant potentiating factor in the activation of the immune response. At the same time, a negative PCR result with a detailed clinical picture of ARVI does not exclude the viral genesis of the disease
Features of laboratory parameters in children with multisystem inflammatory syndrome associated with SARS-CoV-2
Multisystem inflammatory syndrome (MIS-C) is a new childhood disease associated with coronavirus SARS-CoV-2 that manifests itself 3β4 weeks after infection. The development of MIS-C is possible, despite the asymptomatic course of coronavirus infection. The pathogenetic features of MIS-C remain unclear.Β The aim of the study was to investigate the state of peripheral blood parameters in children with multisystem inflammatory syndrome associated with SARS-CoV-2 upon admission to a hospital.Β Material and methods. We examined 52 children with MIS-C from 1 to 14 years old, hospitalized in Children`s Clinical Hospital No. 3 in Novosibirsk in OctoberβDecember 2020. Testing for the isolation of SARS-CoV-2 RNA in scrapings from the nasopharynx and oropharynx was carried out using real time PCR (test systems of DNA-Technology, Russia). The presence of specific IgM and IgG antibodies to SARS-CoV-2 in blood serum was determined by ELISA using Vector-Best kits (Russia).Β Results and discussion. It was noted that SARS-CoV-2 virus RNA was found only in 12.8 % of cases, while specific IgG antibodies in blood serum were detected in 100 % of children. IgM to SARS-CoV-2 antigens were detected in the blood serum only in 53.85 % of children with MIS-C. It has been established that unexpressed leukocytosis was found both in children under the age of 7 and in children of 8β14 years old. However, the leukocytes content in children of the older age group was significantly lower than in children aged 1β7 years. At the same time, in both age groups, some children had extremely low values of the indicator. The causes of severe leukopenia against the background of excessive inflammation remain unclear. Age groups (1β7 and 8β14 years) do not differ statistically significantly in the relative content of lymphocytes and neutrophils. In the study of individual values of the relative content of lymphocytes within the age groups, it was noted that only some children had lymphopenia of varying severity.Β Conclusions. Against the background of minor leukocytosis in children with MIS-C, upon admission to the hospital, there is a tendency to develop both relative and absolute lymphopenia, regardless of age. The study noted an extremely pronounced variability of individual values of the evaluated peripheral blood parameters, regardless of age, which, with similar clinical symptoms, characterized as MIS-C, requires further study in dynamics, taking into account the initial values of the indicators.There is no pronounced relationship between the leukocytes content and specific IgM antibodies to SARS-CoV-2 antigens in the blood
ΠΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΏΡΠΎΠ΄ΡΠΊΡΠΈΠΈ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² ΠΏΡΠΈ Π²ΠΈΡΡΡΠ½ΡΡ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ , ΠΏΡΠΎΡΠ΅ΠΊΠ°ΡΡΠΈΡ Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ
The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children. Materials and research methods. A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No. 6 in Novosibirsk in the first 2 days from the onset of the disease. Two groups were formed β group 1 β patients with diarrheal syndrome and signs of respiratory system damage. Group 2 β children with isolated lesions of the respiratory tract. In all children, the genetic material of viruses was determined by polymerase chain reaction (PCR) in the nose and throat washes. Also, in all children, the determination of serum levels of IL-8, IL-17, IFN-Γ£ by ELISA was performed. Results and discussion. When studying the levels of cytokines (IFN-Γ£, IL-8, IL-17) in patients with signs of gastrointestinal tract lesions in comparison with isolated respiratory manifestations, no statistically significant differences were found. However, when analyzing the levels of the studied parameters in patients with diarrheal syndrome in different age groups, significant differences in the levels of IFN-Γ£ and IL-8 were revealed.Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΡΠ²ΠΈΠ»ΠΎΡΡ ΠΈΠ·ΡΡΠ΅Π½ΠΈΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠ΅ΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π½Π΅ΠΊΠΎΡΠΎΡΡΡ
ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ ΠΊΠ°ΠΊ ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΈΠΌΠΌΡΠ½Π½ΠΎΠ³ΠΎ ΡΠ΅Π°Π³ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΈ Π²ΠΈΡΡΡΠ½ΡΡ
ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΡ
, ΡΠΎΠΏΡΠΎΠ²ΠΎΠΆΠ΄Π°ΡΡΠΈΡ
ΡΡ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Ρ Π΄Π΅ΡΠ΅ΠΉ. ΠΠ°ΡΠ΅ΡΠΈΠ°Π»Ρ ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ. ΠΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠ±ΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠ΅ 92 Π΄Π΅ΡΠ΅ΠΉ Π² Π²ΠΎΠ·ΡΠ°ΡΡΠ΅ ΠΎΡ 3 ΠΌΠ΅ΡΡΡΠ΅Π² Π΄ΠΎ 15 Π»Π΅Ρ Ρ ΠΎΡΡΡΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ Π²ΠΈΡΡΡΠ½ΡΠΌΠΈ ΠΈΠ½ΡΠ΅ΠΊΡΠΈΡΠΌΠΈ, Π³ΠΎΡΠΏΠΈΡΠ°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π½Π½ΡΡ
Π² ΡΡΠ°ΡΠΈΠΎΠ½Π°Ρ ΠΠΠ β6 Π³ΠΎΡΠΎΠ΄Π° ΠΠΎΠ²ΠΎΡΠΈΠ±ΠΈΡΡΠΊΠ° Π² ΠΏΠ΅ΡΠ²ΡΠ΅ Π΄Π²ΠΎΠ΅ ΡΡΡΠΎΠΊ ΠΎΡ Π½Π°ΡΠ°Π»Π° Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ. Π‘ΡΠΎΡΠΌΠΈΡΠΎΠ²Π°Π½Ρ Π΄Π²Π΅ Π³ΡΡΠΏΠΏΡ: I Π³ΡΡΠΏΠΏΠ° β ΠΏΠ°ΡΠΈΠ΅Π½ΡΡ Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ ΠΈ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΡΠ΅ΠΌΡ. ΠΡΡΠΏΠΏΠ° II β Π΄Π΅ΡΠΈ Ρ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΠ΅ΠΌ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ°. Π£ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ ΠΏΡΠΎΠ²Π΅Π»ΠΈ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΌΠ°ΡΠ΅ΡΠΈΠ°Π»Π° Π²ΠΈΡΡΡΠΎΠ² ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΠΎΠ»ΠΈΠΌΠ΅ΡΠ°Π·Π½ΠΎΠΉ ΡΠ΅ΠΏΠ½ΠΎΠΉ ΡΠ΅Π°ΠΊΡΠΈΠΈ (ΠΠ¦Π ) Π² ΡΠΌΡΠ²Π°Ρ
ΠΈΠ· Π½ΠΎΡΠΎ- ΠΈ ΡΠΎΡΠΎΠ³Π»ΠΎΡΠΊΠΈ. Π’Π°ΠΊΠΆΠ΅ Ρ Π²ΡΠ΅Ρ
Π΄Π΅ΡΠ΅ΠΉ Π±ΡΠ»ΠΎ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½ΠΎ ΠΎΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ Π² ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ IL-8, IL-17, IFN-Γ£ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΠ€Π. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. ΠΡΠΈ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΠΈ ΡΡΠΎΠ²Π½Π΅ΠΉ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΡΠΈΡΠΎΠΊΠΈΠ½ΠΎΠ² (IFN-Γ£, IL-8, IL-17) Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ ΠΏΡΠΈΠ·Π½Π°ΠΊΠ°ΠΌΠΈ ΠΏΠΎΡΠ°ΠΆΠ΅Π½ΠΈΡ ΠΆΠ΅Π»ΡΠ΄ΠΎΡΠ½ΠΎ-ΠΊΠΈΡΠ΅ΡΠ½ΠΎΠ³ΠΎ ΡΡΠ°ΠΊΡΠ° Π² ΡΡΠ°Π²Π½Π΅Π½ΠΈΠΈ Ρ ΠΈΠ·ΠΎΠ»ΠΈΡΠΎΠ²Π°Π½Π½ΡΠΌΠΈ ΡΠ΅ΡΠΏΠΈΡΠ°ΡΠΎΡΠ½ΡΠΌΠΈ ΠΏΡΠΎΡΠ²Π»Π΅Π½ΠΈΡΠΌΠΈ ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΡΡ
ΡΠ°Π·Π»ΠΈΡΠΈΠΉ Π²ΡΡΠ²Π»Π΅Π½ΠΎ Π½Π΅ Π±ΡΠ»ΠΎ. ΠΠ΄Π½Π°ΠΊΠΎ ΠΏΡΠΈ Π°Π½Π°Π»ΠΈΠ·Π΅ ΡΡΠΎΠ²Π½Π΅ΠΉ ΠΈΡΡΠ»Π΅Π΄ΡΠ΅ΠΌΡΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ Ρ ΠΏΠ°ΡΠΈΠ΅Π½ΡΠΎΠ² Ρ Π΄ΠΈΠ°ΡΠ΅ΠΉΠ½ΡΠΌ ΡΠΈΠ½Π΄ΡΠΎΠΌΠΎΠΌ Π² ΡΠ°Π·Π»ΠΈΡΠ½ΡΡ
Π²ΠΎΠ·ΡΠ°ΡΡΠ½ΡΡ
Π³ΡΡΠΏΠΏΠ°Ρ
Π²ΡΡΠ²Π»Π΅Π½Ρ Π΄ΠΎΡΡΠΎΠ²Π΅ΡΠ½ΡΠ΅ ΡΠ°Π·Π»ΠΈΡΠΈΡ Π² ΡΡΠΎΠ²Π½Π΅ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ IFN-Γ£ ΠΈ IL-8
Role of myokines in regulation of a power exchange
This review presents new data in respect of polyfunctioning of the muscular system. It is shown that the muscles are able to synthesize and ΡΠ΅ΠΊΡΠ΅ΡΠΈΡΠΎΠ²Π°ΡΡ ΠΌΠΈΠΎΠΊΠΈΠ½Ρ, with auto-couple-and endocrine effects. Is represented by the signal cascade, which runs the transcription of interleukin -6 (Il-6) in the skeletal muscles. For example, IL-6, as an important ΠΌΠΈΠΎΠΊΠΈΠ½Π°, shown its metabolic and Π°Π½ΡΠΈΠ²ΠΎΡΠΏΠ°Π»ΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ effects at physical loads. Considered the issue of the specificity of the cytokine response in muscle contraction. This position is confirmed by the fact that, in ΡΠ½Π΄ΠΎΡΠΎΠΊΡΠ΅ΠΌΠΈΠΈ induced by the introduction of Π»ΠΈΠΏΠΎΠΏΠΎΠ»ΠΈΡΠ°Ρ
Π°ΡΠΈΠ΄Π°, the muscles begin to produce, as the IL-6, and factor of tumor necrosis Ξ±. The biological sense of the rise of production of IL-6 and other ΠΌΠΈΠΎΠΊΠΈΠ½ΠΎΠ² is that ΠΌΠΈΠΎΠΊΠΈΠ½Ρ are the communication system, the input signal of different organs and systems, primarily in the liver, adipose tissue, and immune and endocrine, subordinating their work requirements of long-term muscles flex
The autonomic nervous system in 15β17βyear-old adolescents
Objective: to examine the autonomic nervous system in 15β17βyear-old adolescents.Subjects and methods: 2015 adolescents (1080 boys and 935 girls) aged 15β17 years were examined. The tables developedΒ by A. M. Wayne, cardiointervalography, wedge orthostatic test, and heart rhythm variability were used to evaluate the autonomicΒ nervous system.Β Results. The study showed that the adolescents of both sexes (82% of boys and 84.8% of girls) showed an imbalanced autonomicΒ nervous system as sympathicotonia, vagotony, and vegetative vascular dystonia of mixed-type.Conclusion. The adolescents belong to a group at risk for psychosomatic diseases in the future and therefore require a special followupΒ and prevention activities
Algorithm for automated technology prediction of the risk of chronic somatic pathology in adolescents
A total of 150 Novosibirsk adolescents aged 15 to 17 years of both sexes (75 boys and 75 girls) were examined. The aim of the study was to identify the likely predictors of chronic somatic pathology in 15-17-year-old adolescents according to the data of a comprehensive survey involving the evaluation of physical, mental, and reproductive health. The use of a mathematical modeling method could provide logistic classification models to assess the risk of chrome somatic diseases in adolescents in relation to gender. Psychological factors are of precedence in the development of chronic somatic diseases in adolescents, which is important to be taken into account in the teaching, clinic;) I examination, and rehabilitation of children of this age
The health status of adolescent girls with elevated dehydroepiandrosterone sulfate levels
Objective: to reveal the specific features of somatic, reproductive, and mental health in adolescent girls with elevated dehydroepiandrosterone (DHEA) sulfate levels. One hundred and fifty-nine adolescent girls aged 15 to 18 years from Novosibirsk were examined. A set of studies encompassed clinical examination and examinations by a pediatrician and a gynecologist, abdominal and small pelvic ultrasounds, evaluation of the autonomic nervous system, determination of hormonal profiles, and psychological questionnaire survey. Every two girls had elevated DHEA sulfate levels. The girls with hyperandrogenism had most commonly somatic diseases, as well as psychological features, which require early diagnosis and differential clinical observation to reduce the risk of further reproductive dysfunction
Study of the production of some cytokines in viral infections occurring with diarrheal syndrome in children
The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children. Materials and research methods. A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No. 6 in Novosibirsk in the first 2 days from the onset of the disease. Two groups were formed β group 1 β patients with diarrheal syndrome and signs of respiratory system damage. Group 2 β children with isolated lesions of the respiratory tract. In all children, the genetic material of viruses was determined by polymerase chain reaction (PCR) in the nose and throat washes. Also, in all children, the determination of serum levels of IL-8, IL-17, IFN-Γ£ by ELISA was performed. Results and discussion. When studying the levels of cytokines (IFN-Γ£, IL-8, IL-17) in patients with signs of gastrointestinal tract lesions in comparison with isolated respiratory manifestations, no statistically significant differences were found. However, when analyzing the levels of the studied parameters in patients with diarrheal syndrome in different age groups, significant differences in the levels of IFN-Γ£ and IL-8 were revealed