Visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging

We report a three dimensional (3D) quantitative visualization of a mammalian mitochondrion by coherent x-ray diffractive imaging (CXDI) using synchrotron radiation. The internal structures of a mitochondrion from a mouse embryonic fibroblast cell line (NIH3T3) were visualized by tomographic imaging at approximately 60 nm resolution without the need for sectioning or staining. The overall structure consisted of a high electron density region, composed of the outer and inner membranes and the cristae cluster, which enclosed the lower density mitochondrial matrix. The average mass density of the mitochondrion was about 1.36 g/cm3. Sectioned images of the cristae reveal that they have neither a baffle nor septa shape but were instead irregular. In addition, a high resolution, about 14 nm, 2D projection image was captured of a similar mitochondrion with the aid of strongly scattering Au reference objects. Obtaining 3D images at this improved resolution will allow CXDI to be an effective and nondestructive method for investigating the innate structure of mitochondria and other important life supporting organelles. ? 2017 The Author(s).11Ysciescopu

Tuberculosis verrucosa cutis

Tuberculosis verrucosa cutis is a paucibacillary form of cutaneous tuberculosis caused by exogenous re-infection in previously sensitized individuals. The arms and feet were involved in three cases. Gluteal area was involved in one case. Ziehl-Neelsen and periodic acid-Schiff stains did not demonstrate any acid-fast bacilli. Cultures were positive for Mycobacterium tuberculosis except one case. Lesions of tuberculosis verrucosa cutis improved after anti-tuberculous therapy. There were no active organ and other skin tuberculosis