Expression and alternative splicing of the neural cell adhesion molecule NCAM in human granulosa cells during luteinization

Freshly aspirated human granulosa cells from pre-ovulatory follicles and granulosa cells luteinized in culture possess the neural cell adhesion molecule (NCAM) of approximate molecular mass of 140,000 and NCAM mRNA as confirmed by S1-nuclease protection assays and RT-PCR. Moreover, in the process of luteinization the NCAM isoform pattern is modified. Isoforms containing an insert of 10 amino acids (termed VASE) in the extracellular domain of NCAM were supplemented by alternatively spliced isoforms without this insert. NCAM immunoreactivity, at light and electron microscope levels, was associated with the cell membrane of most granulosa cells which formed clusters. During time in culture an increasing subpopulation of granulosa cells, devoid of NCAM immunoreactivity, spread out and formed monolayers. This differential expression and the alternative splicing of NCAM during luteinization of granulosa cells raise the possibility that NCAM could be involved in folliculogenesis and the formation of the corpus luteum in the human

Search for Associations Containing Young stars (SACY): Chemical tagging IC 2391 & the Argus association

We explore the possible connection between the open cluster IC 2391 and the unbound Argus association identified by the SACY survey. In addition to common kinematics and ages between these two systems, here we explore their chemical abundance patterns to confirm if the two substructures shared a common origin. We carry out a homogenous high-resolution elemental abundance study of eight confirmed members of IC 2391 as well as six members of the Argus association using UVES spectra. We derive spectroscopic stellar parameters and abundances for Fe, Na, Mg, Al, Si, Ca, Ti, Cr, Ni and Ba. All stars in the open cluster and Argus association were found to share similar abundances with the scatter well within the uncertainties, where [Fe/H] = -0.04 +/-0.03 for cluster stars and [Fe/H] = -0.06 +/-0.05 for Argus stars. Effects of over-ionisation/excitation were seen for stars cooler than roughly 5200K as previously noted in the literature. Also, enhanced Ba abundances of around 0.6 dex were observed in both systems. The common ages, kinematics and chemical abundances strongly support that the Argus association stars originated from the open cluster IC 2391. Simple modeling of this system find this dissolution to be consistent with two-body interactions.Comment: 17 pages, 7 figs, accepted for publication in MNRA

Search for associations containing young stars (SACY). V. Is multiplicity universal? Tight multiple systems

Context: Dynamically undisrupted, young populations of stars are crucial to study the role of multiplicity in relation to star formation. Loose nearby associations provide us with a great sample of close ($<$150 pc) Pre-Main Sequence (PMS) stars across the very important age range ($\approx$5-70 Myr) to conduct such research. Aims: We characterize the short period multiplicity fraction of the SACY (Search for Associations Containing Young stars) accounting for any identifiable bias in our techniques and present the role of multiplicity fractions of the SACY sample in the context of star formation. Methods: Using the cross-correlation technique we identified double-lined spectroscopic systems (SB2), in addition to this we computed Radial Velocity (RV) values for our subsample of SACY targets using several epochs of FEROS and UVES data. These values were used to revise the membership of each association then combined with archival data to determine significant RV variations across different data epochs characteristic of multiplicity; single-lined multiple systems (SB1). Results: We identified 7 new multiple systems (SB1s: 5, SB2s: 2). We find no significant difference between the short period multiplicity fraction ($F_\mathrm{m}$) of the SACY sample and that of nearby star forming regions ($\approx$1-2 Myr) and the field ($F_\mathrm{m}\leq$10%) both as a function of age and as a function of primary mass, $M_1$, in the ranges $P$ [1:200 day] and $M_2$ [0.08 $M_{\odot}$-$M_1$]. Conclusions: Our results are consistent with the picture of universal star formation, when compared to the field and nearby star forming regions (SFRs). We comment on the implications of the relationship between increasing multiplicity fraction with primary mass, within the close companion range, in relation to star formation.Comment: 14 pages, 18 figures, published, A&A http://dx.doi.org/10.1051/0004-6361/20142385

Search for associations containing young stars (SACY). VI. Is multiplicity universal? Stellar multiplicity in the range 3-1000 au from adaptive-optics observations

Context. Young loose nearby associations are unique samples of close (<150 pc), young (approx 5-100 Myr) pre-main sequence (PMS) stars. A significant number of members of these associations have been identified in the SACY collaboration. We can use the proximity and youth of these members to investigate key ingredients in star formation processes, such as multiplicity. Aims. We present the statistics of identified multiple systems from 113 confirmed SACY members. We derive multiplicity frequencies, mass-ratio, and physical separation distributions in a consistent parameter space, and compare our results to other PMS populations and the field. Methods. We have obtained adaptive-optics assisted near-infrared observations with NACO (ESO/VLT) and IRCAL (Lick Observatory) for at least one epoch of all 113 SACY members. We have identified multiple systems using co-moving proper-motion analysis and using contamination estimates. We have explored ranges in projected separation and mass-ratio of a [3-1000 au], and q [0.1-1], respectively. Results. We have identified 31 multiple systems (28 binaries and 3 triples). We derive a multiplicity frequency (MF) of MF_(3-1000au)=28.4 +4.7, -3.9% and a triple frequency (TF) of TF_(3-1000au)=2.8 +2.5, -0.8% in the separation range of 3-1000 au. We do not find any evidence for an increase in the MF with primary mass. The estimated mass-ratio of our statistical sample (with power-law index gamma=-0.04 +/- 0.14) is consistent with a flat distribution (gamma = 0). Conclusions. We show further similarities (but also hints of discrepancies) between SACY and the Taurus region: flat mass-ratio distributions and statistically similar MF and TF values. We also compared the SACY sample to the field (in the separation range of 19-100 au), finding that the two distributions are indistinguishable, suggesting a similar formation mechanism.Comment: 16 Pages, accepted in A&A 28 May 201

Search for associations containing young stars (SACY) VII. New stellar and substellar candidate members in the young associations

The young associations offer us one of the best opportunities to study the properties of young stellar and substellar objects and to directly image planets thanks to their proximity ($<$200 pc) and age ($\approx$5-150 Myr). However, many previous works have been limited to identifying the brighter, more active members ($\approx$1 M$_\odot$) owing to photometric survey sensitivities limiting the detections of lower mass objects. We search the field of view of 542 previously identified members of the young associations to identify wide or extremely wide (1000-100,000 au in physical separation) companions. We combined 2MASS near-infrared photometry ($J$, $H$, $K$) with proper motion values (from UCAC4, PPMXL, NOMAD) to identify companions in the field of view of known members. We collated further photometry and spectroscopy from the literature and conducted our own high-resolution spectroscopic observations for a subsample of candidate members. This complementary information allowed us to assess the efficiency of our method. We identified 84 targets (45: 0.2-1.3 M$_\odot$, 17: 0.08-0.2 M$_\odot$, 22: $<$0.08 M$_\odot$) in our analysis, ten of which have been identified from spectroscopic analysis in previous young association works. For 33 of these 84, we were able to further assess their membership using a variety of properties (X-ray emission, UV excess, H$_\alpha$, lithium and K I equivalent widths, radial velocities, and CaH indices). We derive a success rate of 76-88% for this technique based on the consistency of these properties. Once confirmed, the targets identified in this work would significantly improve our knowledge of the lower mass end of the young associations. Additionally, these targets would make an ideal new sample for the identification and study of planets around nearby young stars.Comment: 28 pages, 24 figures, accepted in A&

A brown dwarf companion to the intermediate-mass star HR6037

In the course of an imaging survey we have detected a visual companion to the intermediate-mass star HR 6037. In this letter, we present two epoch observations of the binary with NACO/VLT, and near-IR spectroscopy of the secondary with ISAAC/VLT. The NACO observations allow us to confirm HR 6037B as a co-moving companion. Its J and H band ISAAC spectra suggest the object has an spectral type of M9+-1, with a surface gravity intermediate between that of 10 Myr dwarfs and field dwarfs with identical spectral type. The comparison of its Ks-band photometry with evolutionary tracks allows us to derive a mass, effective temperature, and surface gravity of 62+-20 MJup, Teff = 2330+-200 K, and log g = 5.1+-0.2, respectively. The small mass ratio of the binary, -0.03, and its long orbital period, -5000 yr, makes HR 6037 a rare and uncommon binary system.Comment: (5 pages, 4 figures, accepted for publication in A&A Letters

A spectroscopic survey of the youngest field stars in the solar neighbourhood. I. The optically bright sample

We present the first results of a ground-based programme conducted on 1-4m class telescopes. Our sample consists of 1097 active and presumably young stars, all of them being optical counterparts of RASS X-ray sources in the northern hemisphere. We concentrate on the 704 optically brightest (V_Ticho<=9.5 mag) candidates. We acquired high-res spectroscopy in the Halpha/Li spectral regions for 426 of such stars without relevant literature data. We describe the sample and the observations and we start to discuss its physical properties. We used a cross-correlation technique and other tools to derive accurate radial/rotational velocities and to perform a spectral classification for both single and SB2 stars. The spectral subtraction technique was used to derive chromospheric activity levels and Li abundances. We estimated the fraction of young single stars and multiple systems in stellar soft X-ray surveys and the contamination by more evolved systems, like RS CVn's. We classified stars on the basis of Li abundance and give a glimpse of their sky distribution. The sample appears to be a mixture of young Pleiades-/Hyades- like stars plus an older Li-poor population (~1-2 Gyr). 7 stars with Li abundance compatible with the age of IC 2602 (~30 Myr) or younger were detected as well, although 2 appear to be Li-rich giants. The discovery of a large number of Li-rich giants is another outcome of this survey. The contamination of soft X-ray surveys by old systems in which the activity level is enhanced by tidal synchronisation is not negligible, especially for K-type stars. 5 stars with Li content close to the primordial abundance are probably associated with known moving groups in the solar neighbourhood. Some of them are PTTS candidates according to their positions in the HR diagram.Comment: 16 pages, 12 figures, 6 tables; 2 figures and 2 tables in electronic form only. Paper accepted by Astronomy and Astrophysic

Multiplicity and Optical Excess Across the Substellar Boundary in Taurus

We present the results of a high-resolution imaging survey of 22 brown dwarfs and very low mass stars in the nearby (~145 pc) young (~1-2 Myr) low-density star-forming region Taurus-Auriga. We obtained images with the Advanced Camera for Surveys/High Resolution Channel on HST through the F555W (V), F775W (i'), and F850LP (z') filters. This survey confirmed the binarity of MHO-Tau-8 and discovered a new candidate binary system, V410-Xray3, resulting in a binary fraction of 9+/-5% at separations >4 AU. Both binary systems are tight (<10 AU) and they possess mass ratios of 0.75 and 0.46, respectively. The binary frequency and separations are consistent with low-mass binary properties in the field, but the mass ratio of V410-Xray3 is among the lowest known. We find that the binary frequency is higher for very low mass stars and high-mass brown dwarfs than for lower-mass brown dwarfs, implying either a decline in frequency or a shift to smaller separations for the lowest mass binaries. Combining these results with multiplicity statistics for higher-mass Taurus members suggests a gradual decline in binary frequency and separation toward low masses. The implication is that the distinct binary properties of very low-mass systems are set during formation and that the formation process is similar to the process which creates higher-mass stellar binaries, but occurs on a smaller scale. We show that there are no planets or very low-mass brown dwarfs with mass >3 M_J at projected separation >40 AU orbiting any of the Taurus members in our sample. We identify several BDs with significant (>1 mag) V-band excesses. The excesses appear to be correlated with signatures of accretion, and if attributed to accretion luminosity, may imply mass accretion rates several orders of magnitude above those inferred from line-profile analyses. (abridged)Comment: Accepted for publication in ApJ; 15 pages, 8 figures in emulateapj forma

Very Low Mass Stars and Brown Dwarfs in Taurus-Auriga

We present high resolution optical spectra obtained with the HIRES spectrograph on the Keck I telescope of low mass T Tauri stars and brown dwarfs (LMTTs) in Taurus-Auriga. Of particular interest is the previously classified "continuum T Tauri star" GM Tau, which has a spectral type of M6.5 and a mass just below the stellar/substellar boundary. None of the LMTTs in Taurus are rapidly rotating (vsini < 30 km/s), unlike low mass objects in Orion. Many of the slowly rotating, non-accreting stars and brown dwarfs exhibit prominent H-alpha emission (EWs of 3 - 36 A), indicative of active chromospheres. We demonstrate empirically that the full-width at 10% of the H-alpha emission profile peak is a more practical and possibly more accurate indicator of accretion than either the equivalent width of H-alpha or optical veiling: 10%-widths > 270 km/s are classical T Tauri stars (i.e. accreting), independent of stellar spectral type. Although LMTTs can have accretion rates comparable to that of more typical, higher-mass T Tauri stars (e.g. K7-M0), the average mass accretion rate appears to decrease with decreasing mass. The diminished frequency of accretion disks for LMTTs, in conjunction with their lower, on average, mass accretion rates, implies that they are formed with less massive disks than higher-mass T Tauri stars. The radial velocities, circumstellar properties and known binaries do not support the suggestion that many of the lowest mass members of Taurus have been ejected from higher stellar density regions within the cloud. Instead, LMTTs appear to have formed and are evolving in the same way as higher-mass T Tauri stars, but with smaller disks and shorter disk lifetimes.Comment: 27 pages, plus 8 figures, accepted for publication in Ap

Planets around stars in young nearby associations *** radial Velocity searches: a feasibility study, and first results

Stars in young nearby associations are the only targets allowing giant planet searches at all separations in the near future, by coupling indirect techniques such as radial velocity and deep imaging. These stars are first priorities targets for the forthcoming planets imagers on 10-m class telescopes. Young stars rotate more rapidly and are more active than their older counterparts. Both effects can limit the capability to detect planets using RV. We wish to explore the planet detection capabilities of a representative sample of stars in close and young associations with radial velocity data and explore the complementarity between this technique and direct imaging. We observed 26 such targets with spectral types from A to K and ages fromComment: submitte