Relation between Endothelial Nitric Oxide Synthase Genotypes and Oxidative Stress Markers in Larynx Cancer

Nitric oxide synthase (eNOS/NOS3) is responsible for the endothelial synthesis of nitric oxide (NO•). G894T polymorphism leads to the amino acid substitution from Glu298Asp that causes lower NOS3 activity and basal NO• production in NOS3 894T (298Asp) allele carriers compared with the GG homozygotes. NO• acts as an antioxidant protecting against Fenton’s reaction which generates highly reactive hydroxyl radicals. Allelic variation of NOS3 may influence an individual’s risk of laryngeal cancer (LC). In the current study we have examined the possible relationship between NOS3 G894T genotypes and various systemic oxidative damage markers such as protein carbonyl, advanced oxidation protein products, Cu, Zn-superoxide dismutase, thiol group fractions, and lipid hydroperoxides in LC patients. Genotyping was carried out by PCR-RFLP. In LC patients with TT genotype, Cu, Zn-superoxide dismutase activities and nonprotein thiol levels were significantly higher than the controls. In patients with GT and GG genotype, high levels of lipid hydroperoxides showed statistical significance when compared to controls. Our results indicate a potential relationship among G894T polymorphism of NOS3, and impaired redox homeostasis. Further studies are required to determine the role of NOS3 gene polymorphism and impaired plasma redox homeostasis

Genetic and environmental parameters and trends for milk production of Holstein cattle in Turkey

Data from 4143 Holstein cattle from three herds in Turkey were used to study the influences of genetic and environmental factors on milk production traits as well as genetic and environmental trends over 20 years from 1987 to 2006. First lactation mean values for 305-day milk yield, actual lactation milk yield, and lactation length were 6222±35.8 kg, 6651±42.6 kg, and 327±1.3 days. Second and third 305-day lactation yields were proportionately 8% and 11% greater, respectively. The effects of herd and calving year were the largest sources of variation for all traits. Heritabilities of actual lactation milk yield, 305-day milk yield, and lactation length for first lactation records were 0.24±0.007, 0.23±0.007, and 0.08±0.035. Estimates of variances and genetic parameters were consistent with estimates reported throughout the literature. Trends were inconsistent with some phenotypic increase in yields during the period between 2001 and 2005. This improvement was associated with increased environment parameter estimates during that period. No consistent genetic improvement was observed