Drosophila RecQ4 Is Directly Involved in Both DNA Replication and the Response to UV Damage in S2 Cells.

The RecQ4 protein shows homology to both the S.cerevisiae DNA replication protein Sld2 and the DNA repair related RecQ helicases. Experimental data also suggest replication and repair functions for RecQ4, but the precise details of its involvement remain to be clarified.Here we show that depletion of DmRecQ4 by dsRNA interference in S2 cells causes defects consistent with a replication function for the protein. The cells show reduced proliferation associated with an S phase block, reduced BrdU incorporation, and an increase in cells with a subG1 DNA content. At the molecular level we observe reduced chromatin association of DNA polymerase-alpha and PCNA. We also observe increased chromatin association of phosphorylated H2AvD--consistent with the presence of DNA damage and increased apoptosis.Analysis of DmRecQ4 repair function suggests a direct role in NER, as the protein shows rapid but transient nuclear localisation after UV treatment. Re-localisation is not observed after etoposide or H₂O₂ treatment, indicating that the involvement of DmRecQ4 in repair is likely to be pathway specific.Deletion analysis of DmRecQ4 suggests that the SLD2 domain was essential, but not sufficient, for replication function. In addition a DmRecQ4 N-terminal deletion could efficiently re-localise on UV treatment, suggesting that the determinants for this response are contained in the C terminus of the protein. Finally several deletions show differential rescue of dsRNA generated replication and proliferation phenotypes. These will be useful for a molecular analysis of the specific role of DmRecQ4 in different cellular pathways

Chandra X-ray Observation of a Mature Cloud-Shock Interaction in the Bright Eastern Knot Region of Puppis A

We present Chandra X-ray images and spectra of the most prominent cloud-shock interaction region in the Puppis A supernova remnant. The Bright Eastern Knot (BEK) has two main morphological components: (1) a bright compact knot that lies directly behind the apex of an indentation in the eastern X-ray boundary and (2) lying 1' westward behind the shock, a curved vertical structure (bar) that is separated from a smaller bright cloud (cap) by faint diffuse emission. Based on hardness images and spectra, we identify the bar and cap as a single shocked interstellar cloud. Its morphology strongly resembles the ``voided sphere'' structures seen at late times in Klein et al.'s experimental simulations of cloud-shock interactions, when the crushing of the cloud by shear instabilities is well underway. We infer an interaction time of roughly 3 cloud-crushing timescales, which translates to 2000-4000 years, based on the X-ray temperature, physical size, and estimated expansion of the shocked cloud. This is the first X-ray identified example of a cloud-shock interaction in this advanced phase. Closer to the shock front, the X-ray emission of the compact knot in the eastern part of the BEK region implies a recent interaction with relatively denser gas, some of which lies in front of the remnant. The complex spatial relationship of the X-ray emission of the compact knot to optical [O III] emission suggests that there are multiple cloud interactions occurring along the line of sight.Comment: 22 pages LaTeX with multiple figures, to appear in Ap

Discovery of the compact X-ray source inside the Cygnus Loop

We detected an X-ray compact source inside the Cygnus Loop during the observation project of the whole Cygnus Loop with the ASCA GIS. The source intensity is 0.11 c s$^{-1}$ for GIS and 0.15 c s$^{-1}$ for SIS, which is the strongest in the ASCA band. The X-ray spectra are well fitted by a power law spectrum of a photon index of \error{-2.1}{0.1} with neutral H column of (\error{3.1}{0.6})${\rm \times 10^{21} cm^{-2}}$. Taking into account the interstellar absorption feature, this source is X-ray bright mainly above 1 keV suggesting either an AGN or a rotating neutron star. So far, we did not detect intensity variation nor coherent pulsation mainly due to the limited observation time. There are several optical bright stellar objects within the error region of the X-ray image. We carried out the optical spectroscopy for the brightest source (V=+12.6) and found it to be a G star. The follow up deep observation both in optical and in X-ray wavelengths are strongly required.Comment: Accepted for Publications of the Astronomical Society of Japan 17 pages, 3 figur

X-ray Emitting Ejecta in Puppis A Observed with Suzaku

We report the detection and localization of X-ray emitting ejecta in the middle-aged Galactic supernova remnant Puppis A using five observations with the Suzaku X-ray Imaging Spectrometer to survey the eastern and middle portions of the remnant. A roughly 3' by 5', double-peaked region in the north center is found to be highly enriched in Si and other elements relative to the rest of the remnant. The X-ray fitted abundances are otherwise well below the solar values. While the ejecta-enhanced regions show some variation of relative element abundances, there is little evidence for a very strong enhancement of one element over the others in the imaged portion of the remnant, except possibly for a region of O and Ne enhancement in the remnant's south center. There is no spatial correlation between the compact [O III] emitting ejecta knots seen optically and the abundance enhancements seen in X-rays, although they are located in the same vicinity. The map of fitted column density shows strong variations across the remnant that echo earlier X-ray spectral hardness maps. The ionization age (as fitted for single temperature models) is sharply higher in a ridge behind the northeast-east boundary of the remnant, and is probably related to the strong molecular cloud interaction along that boundary. The temperature map, by comparison, shows relatively weak variations.Comment: 25 pages latex, 6 postscript figures; ApJ, in pres

Resolved 24.5 micron emission from massive young stellar objects

Massive young stellar objects (MYSO) are surrounded by massive dusty envelopes. Our aim is to establish their density structure on scales of ~1000 AU, i.e. a factor 10 increase in angular resolution compared to similar studies performed in the (sub)mm. We have obtained diffraction-limited (0.6") 24.5 micron images of 14 well-known massive star formation regions with Subaru/COMICS. The images reveal the presence of discrete MYSO sources which are resolved on arcsecond scales. For many sources, radiative transfer models are capable of satisfactorily reproducing the observations. They are described by density powerlaw distributions (n(r) ~ r^(-p)) with p = 1.0 +/-0.25. Such distributions are shallower than those found on larger scales probed with single-dish (sub)mm studies. Other sources have density laws that are shallower/steeper than p = 1.0 and there is evidence that these MYSOs are viewed near edge-on or near face-on, respectively. The images also reveal a diffuse component tracing somewhat larger scale structures, particularly visible in the regions S140, AFGL 2136, IRAS 20126+4104, Mon R2, and Cep A. We thus find a flattening of the MYSO envelope density law going from ~10 000 AU down to scales of ~1000 AU. We propose that this may be evidence of rotational support of the envelope (abridged).Comment: 21 pages, accepted for A&

Crystalline Silicate Feature of the Vega-like star HD145263

We have observed the 8-13 $\mu$m spectrum (R$\sim$250) of the Vega-like star candidate HD145263 using Subaru/COMICS. The spectrum of HD145263 shows the broad trapezoidal silicate feature with the shoulders at 9.3 $\mu$m and 11.44 $\mu$m, indicating the presence of crystalline silicate grains. This detection implies that crystalline silicate may also be commonly present around Vega-like stars. The 11.44 $\mu$m feature is slightly shifted to a longer wavelength compared to the usual 11.2-3 $\mu$m crystalline forsterite feature detected toward Herbig Ae/Be stars and T Tauri stars. Although the peak shift due to the effects of the grain size can not be ruled out, we suggest that Fe-bearing crystalline olivine explains the observed peak wavelength fairly well. Fe-bearing silicates are commonly found in meteorites and most interplanetary dust particles, which originate from planetesimal-like asteroids. According to studies of meteorites, Fe-bearing silicate must have been formed in asteroidal planetesimals, supporting the scenario that dust grains around Vega-like stars are of planetesimal origin, if the observed 11.44 $\mu$m peak is due to Fe-bearing silicates.Comment: accepted for Publication in ApJ