Rheumatoid arthritis synovial tissue (RA ST) had higher pPyk2 immunopositive cells compared to osteoarthritis (OA) ST

<p><b>Copyright information:</b></p><p>Taken from "Differential expression of the FAK family kinases in rheumatoid arthritis and osteoarthritis synovial tissues"</p><p>http://arthritis-research.com/content/9/5/R112</p><p>Arthritis Research & Therapy 2007;9(5):R112-R112.</p><p>Published online 26 Oct 2007</p><p>PMCID:PMC2212559.</p><p></p> RA (×200), compared to OA (×200) and normal donor (ND) (×200). is the quantification data obtained from figure a and b. Bars represent mean and SEM. RA ST fibroblasts or peripheral blood differentiated MΦs were stimulated with TNF-α (10 ng/ml) or IL1-β (10 ng/ml) from 0–120 min. Cell lysates were examined by western blot analysis for pPyk2 or Pyk2 expression. The results are representative of three experiments. Inflam, inflammatory score; Vasc, vascularity score; Lining, ST lining cell layer; Mac, subsynovial MΦs

PPLCγ immunostaining is higher in rheumatoid arthritis synovial tissue (RA ST) in comparison to osteoarthritis (OA) and normal donor (ND) ST

<p><b>Copyright information:</b></p><p>Taken from "Differential expression of the FAK family kinases in rheumatoid arthritis and osteoarthritis synovial tissues"</p><p>http://arthritis-research.com/content/9/5/R112</p><p>Arthritis Research & Therapy 2007;9(5):R112-R112.</p><p>Published online 26 Oct 2007</p><p>PMCID:PMC2212559.</p><p></p> RA (×200), compared to OA (×200) and ND (×200). The quantification data obtained from a, b and c. Bars represent mean and SEM. Inflam, inflammatory score; Vasc, vascularity score; Lining, ST lining cell layer; Mac, subsynovial MΦs

Histological scores of ankle sections from wild-type (Wt) and Bid-/- mice

<p><b>Copyright information:</b></p><p>Taken from "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis"</p><p>http://arthritis-research.com/content/9/3/R49</p><p>Arthritis Research & Therapy 2007;9(3):R49-R49.</p><p>Published online 17 May 2007</p><p>PMCID:PMC2206343.</p><p></p> Bid-/- mice have increased inflammation and joint destruction compared to Wt mice. Ankles isolated from mice (Wt, = 9; Bid-/- = 7) were prepared as described in Figure 2. Ankle sections were evaluated and scored by a pathologist blinded to the study as described in the Materials and methods section. Values represent the mean ± standard error of ankles/time point, which were compared by Student's -test. Increased numbers of lymphocytes and polymorphonuclear (PMNs) cells in inflamed Bid-/- joints. Ankles were prepared as described above. Values represent the mean ± standard error of ankles/time point, which were compared by Student's -test. Arthritic Bid-/- mice have more macrophages in the pannus and in the whole joint. Ankles were examined for F4/80 antigen as described in Materials and methods. The number of positive cells for F4/80 in pannus, synovial lining, and whole joint was determined by a pathologist blinded to the study. Values represent the mean ± standard error of ankles/time point, which were compared by Student's -test

Loss of Bid does not alter the cytokine and chemokine milieu of the joint

<p><b>Copyright information:</b></p><p>Taken from "Pro-apoptotic Bid is required for the resolution of the effector phase of inflammatory arthritis"</p><p>http://arthritis-research.com/content/9/3/R49</p><p>Arthritis Research & Therapy 2007;9(3):R49-R49.</p><p>Published online 17 May 2007</p><p>PMCID:PMC2206343.</p><p></p> Pro-inflammatory cytokine production in ankle joints following transfer of K/BxN serum. Untreated wild-type (Wt) and Bid-/- mice were euthanized at three, five, or seven days post-serum transfer. Ankles from each mouse (days 3, = 6 (Wt) and n= 8 (Bid-/-); day 5, = 10; day 7, = 12 (Wt) and = 8 (Bid-/-)) were isolated, snap frozen, ground into a fine powder, lysed, and examined for production of tumor necrosis factor (TNF)α and IL-1β using sandwich ELISAs. Chemokine production in ankle joints following transfer of K/BxN serum. Ankles lysates as described above were examined for production of CXC chemokine (KC) and monocyte chemoattractant protein (MCP)-1 using ELISA. Data are shown as μg/μl per joint. Values represent the mean ± standard error, which were compared by Student's -test