2 research outputs found
Adaptation of Scheffersomyces stipitis to hardwood spent sulfite liquor by evolutionary engineering
Hardwood spent sulfite liquor (HSSL) is a by-product of acid sulfite pulping process that is rich in
xylose, a monosaccharide that can be fermented to ethanol by Scheffersomyces stipitis. However, HSSL also contains
acetic acid and lignosulfonates that are inhibitory compounds of yeast growth. The main objective of this study
was the use of an evolutionary engineering strategy to obtain variants of S. stipitis with increased tolerance to HSSL
inhibitors while maintaining the ability to ferment xylose to ethanol