Bifunctionalised long-wavelength fluorescent probes for biological applications

The synthesis of benzo[a]phenoxazinium chlorides which are bifunctionalised in position 2 with 4-ethoxy-4-oxobutoxyl, 3-hydroxypropoxyl or 3-chloropropoxyl groups, and in position 9 with the (aminopropyl)amino group, was efficiently performed. The covalent labeling of valine was carried out by using one of the new fluorophores obtained. Photophysical studies in the homogeneous media of ethanol, distilled water and at simulated physiological conditions revealed that all the compounds absorbed and emitted from 610 to 651 nm.Fundação para a Ciência e Tecnologia (FCT, Portugal) - financial support to the NMR portuguese network (PTNMR, Bruker Avance III 400-Univ. Minho), FCT and FEDER (European Fund for Regional Development)-COMPETE-QREN-EU - financial support to the Research Centre, CQ/UM [PEst-C/QUI/UI0686/2011 (FCOMP-01-0124-FEDER-022716)]

DNA fluorescence probes based on side-chain chlorinated benzo[a]phenoxazinium chlorides

Several side chain chlorinated benzo[a]phenoxazinium chlorides functionalized with various types of terminals at the 5- and 9-amino positions of the heteroaromatic ring were used in photophysical studies with DNA. It was found that the functionalised terminal has a dramatic influence on the type of interaction with the amino group at the 9-amino position greatly promoting intercalation.Thanks are due to Fundação para a Ciência e Tecnologia (Portugal) for its financial support of a BPD to B. R. Raju (SFRH/BPD/62881/2009), CQUM and CFUM

Tuberculosis in cattle slaughtered in the intermediate geographic region of Caicó, State of Rioi Grande do Norte, Brazil.

The bovine farming constitutes an economic and social activity of great importance for the State of Rio Grande do Norte, contributing for the creation of jobs and generation of income. However, factors related to the low technification of the activity on the part of the producers, the occurrence of a wide and intense drought in the last years and the presence of infectious diseases have caused significant damages to the breeders of animals in the State. Infectious diseases include tuberculosis, which is a zoonotic infection caused by Mycobacterium bovis, the primary host of which is cattle, but several domestic and wild mammal species, including humans, are also susceptible. The objective of the study was to determine the occurrence of tuberculosis in bovine slaughtered in two public slaughterhouses of the intermediate geographic region of Caicó, State of Rio Grande do Norte. Between November 2017 and August 2018, a total of 11,616 bovine carcasses underwent routine post-mortem inspection, with lesions suggestive of tuberculosis in one animal (0.009%). Samples of tissues located in the lung, heart and spleen were submitted to bacteriological culture and molecular diagnosis

Elaboração de snacks de milho e de farinha da torta semi-desengordurada de gergelim.

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Prospecção de moléculas de extratos vegetais do bioma cerrado para o controle de Colletotrichum gossypii var. cephalosporioides e Meloidogyne incognita.

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Agroforestry systems of high natural and cultural value in Europe: structure, management, goods and services

info:eu-repo/semantics/publishedVersio