Impact of endophyte inoculation on the morphological identity of cultivars of Lolium perenne (L) and Festuca arundinacea (Schreb.)

Publication history: Accepted - 9 April 2020; Published online - 5 May 2020Grass endophytes have been shown to confer enhanced environmental resilience to symbiont cultivars with reports of modified growth. If inoculating with an endophyte (E+) made an accession morphologically distinct from its registered endophyte free (E−) accession, there could be protection and ownership issues for testing authorities and breeders. This study investigated if, in official Plant Breeders Rights (PBR) field trials, the morphological characteristics of E+and E− accessions of perennial ryegrass and tall fescue cultivars were sufficiently modified to designate them as mutually distinct and also distinct from their definitive accessions (Def), held by the testing authorities. Testing perennial ryegrass on 17 characters at 2 sites generated 48,960 observations and for tall fescue on 9 characters at 1 site, 12,960 observations (each for 3 accessions of 4 cultivars × 60 plants × 2 growing cycles). Distinctness required a p < 0.01 difference in a single character from the combined over years analysis (COYD). A few significant differences were recorded between E− and E+accessions. Cultivar Carn E+ was smaller than Carn E− for Infloresence Length (p < 0.01) in both years but COYD analysis (p < 0.05) was insufficient to declare distinctiveness. Overall, the number of observed differences between E−/E+ accessions was less or similar to the number expected purely by chance. In contrast, comparisons between Def and E− or E+ accessions showed a number of significant differences that were substantially more numerous than expected by chance. These results showed no conclusive evidence of endophyte inclusion creating false PBR distinctions but unexpectedly, several E− and E+ accessions were distinguished from their official definitive stock.This study was jointly funded by the EU Community Plant Variety Office, Angers, France and Euroseeds, Brussels, Belgium

NK cells and cancer: you can teach innate cells new tricks

Natural killer (NK) cells are the prototype innate lymphoid cells endowed with potent cytolytic function that provide host defence against microbial infection and tumours. Here, we review evidence for the role of NK cells in immune surveillance against cancer and highlight new therapeutic approaches for targeting NK cells in the treatment of cancer

Stress-driven increase in proline levels, and not proline levels themselves, correlates with the ability to withstand excess salt in a group of 17 Italian rice genotypes

In most plant species, a rapid increase in free proline content occurs following exposure to hyperosmotic stress conditions. However, inconsistent results were reported concerning the role of such an increase on the plant response to water shortage or excess salt. Therefore, the possibility that proline accumulation may help the cell to withstand stress conditions, or that it simply represents a stress marker, is still a matter of debate. A possible relationship between proline accumulation and salt tolerance was investigated in a set of 17 Italian rice varieties. Rice seedlings were exposed to increasing salt concentrations during germination and early growth. The resulting levels of free proline were measured separately in shoots and roots and compared to those in untreated controls. Results were related to the corresponding ability of a given genotype to tolerate stress conditions. Neither absolute proline levels in untreated or in salt-stressed seedlings showed a straightforward relationship to the relative tolerance to salt, estimated as conductivity values able to reduce growth by 10 or 50%. Conversely, a highly significant correlation was found between the increase in proline levels in shoots and the ability to withstand stress. The results strengthen a recent hypothesis suggesting than an increase in proline metabolic rates, more than the resulting proline content, may help the cell to counteract the effects of abiotic stress conditions

Economic Dispatch for on-line operation of grid-connected microgrids

In this paper, a control strategy for real-time operation of a master-slave controlled microgrid is developed. The basic idea of this control strategy is to schedule all dispatchable energy sources available into a microgrid to minimize its operational costs. Control actions are centrally evaluated by solving a two-stage optimization problem formulated to take place on two different time-scales: in the day-ahead and in the real-time. The first one provides a 24-hour plan in advance. It mainly draws up the active power levels that Distributed Energy Resources (DERs) should provide for each quarter hour of the next day by taking into account energy prices of the day-ahead energy market, the forecasted energy production of non-dispatchable renewables and loads. The real-time optimization problem updates the active power set-points of DERs in order to minimize as much as possible the real-time deviations between the actual power exchanged with the utility grid and its scheduled value. The effectiveness of the proposed methodology has been experimentally tested on an actual microgrid

On the Impact of Heat Pumps Electric Load on the Power Consumption of Lampedusa

The present paper reports the results of a simulation study regarding the impact of the adoption of heat pumps for domestic hot water production in the island of Lampedusa (Italy). In the study, it has been assumed to replace electric storage water heaters with heat pumps at every dwelling of the about four thousand domestic end-users of the island and, in addition, to control the heat pump operation in order to enhance some parameters that characterize the quality of the generation and distribution system of the island (power peak, energy losses and voltage drop). According to the results of the simulations, while the replacement of electric storage water heaters with heat pumps is able to positively impact the island power system, the control of heat pumps, even if in great number, does not generate any evident additional advantage for the power system operation. These results are significant for better understanding potentiality and limits of Demand Response and Aggregation in the presence of high-efficiency devices

Experimental results on the economic management of a smart microgrid

The aim of this paper is to assess economic benefits deriving from the adoption of a smart microgrid. To this end, a case study consisting of 250 houses connected to the distribution network through a MV/LV transformer substation has been investigated and implemented in the experimental microgrid built at the Polytechnic University of Bari, Italy. The analysis has been performed by comparing three different energy providers: the utility grid, the utility grid with the inclusion of non-programmable renewables generators, and the inclusion of such residential loads into a smart microgrid. The obtained results demonstrated that with the adoption of a smart microgrid significant costs savings can be obtained

Effects of Demand Side Management on the Operation of an Isolated LV Microgrids

The work presents the results of a study regarding the impact of Demand Side Management actions on flexible loads distributed at the domestic end-users' facilities in an isolated low voltage microgrid. Aim of the research study is to evaluate how the implementation of local control actions on the end-user's side can impact on the operation of the isolated microgrid. The Demand Side Management is applied by each domestic user to optimize the operation of its own resources following well-defined operative steps. The study is carried out implementing a suitable model of the microgrid in Neplan environment, while the daily controlled load profiles of the end-users are evaluated using the Montecarlo approach implemented in Matlab. The results of the study are useful to the DSOs which will face all the issues rising in a fully-automated environment

Transitions from grid-connected to island operation of Smart Microgrids

In this paper a control methodology is presented to perform a bumpless transition from the on-grid to the offgrid of a smart microgrid. The derived controller manages the internal production in order to get to zero the tie-line active and reactive power flows so that the opening of the tie line breaker does not produce significant perturbations. Experimental tests performed on the microgrid built at the Polytechnic University of Bari demonstrated the effectiveness of the proposed control strategy

Preoperative chemotherapy with methotrexate and cis-diammine-dichloro-platinum II in osteosarcoma

Preoperative chemotherapy with methotrexate and cis-diammine-dichloro-platinum II in osteosarcoma