Método 'Impactos-Nanotec' para avaliação dos impactos ambientais: estudo de caso das nanotecnologias na agricultura.

Resumo: A Nanotecnologia está baseada na crescente capacidade da tecnologia moderna de manipular átomos e partículas em nanoescala, com aplicações em diversas áreas de atuação desde a medicina, meio ambiente e agricultura. Apesar das nanotecnologias apresentarem propriedades físicas específicas, a avaliação dos impactos associados ao seu emprego e liberação no meio ambiente ainda não é uma prática corrente. Neste cenário, o presente trabalho propõe o estudo de caso de nanopartículas na agricultura, através do levantamento de indicadores, utilizando uma metodologia empregada para estudo de transgênicos, adaptada para um novo software de avaliação de risco de nanoproduto

Nanotecnologia na agricultura: prospecção dos indicadores de impactos ambientais e sociais.

Resumo: A Nanotecnologia está baseada na crescente capacidade da tecnologia moderna de manipular átomos e partículas em nanoescala, com aplicações em diversas áreas de atuação, desde a medicina, meio ambiente e agricultura. Apesar das nanotecnologias apresentarem propriedades físicas específicas, a avaliação dos impactos associados ao seu emprego e liberação no meio ambiente ainda não é uma prática corrente. Neste cenário, o presente trabalho propõe um estudo de caso sobre as nanopartículas na agricultura, através da formulação de indicadores de impacto a partir de levantamento da literatura científica

Exploring the utility of human DNA methylation arrays for profiling mouse genomic DNA

AbstractIllumina Infinium Human Methylation (HM) BeadChips are widely used for measuring genome-scale DNA methylation, particularly in relation to epigenome-wide association studies (EWAS) studies. The methylation profile of human samples can be assessed accurately and reproducibly using the HM27 BeadChip (27,578 CpG sites) or its successor, the HM450 BeadChip (482,421 CpG sites). To date no mouse equivalent has been developed, greatly hindering the application of this methodology to the wide range of valuable murine models of disease and development currently in existence. We found 1308 and 13,715 probes from HM27 and HM450 BeadChip respectively, uniquely matched the bisulfite converted reference mouse genome (mm9). We demonstrate reproducible measurements of DNA methylation at these probes in a range of mouse tissue samples and in a murine cell line model of acute myeloid leukaemia. In the absence of a mouse counterpart, the Infinium Human Methylation BeadChip arrays have utility for methylation profiling in non-human species

Validation of the Italian version of the Non-Communicating Children's Pain Checklist-Postoperative Version

BACKGROUND: This study evaluated the validity and reliability of the Italian version of the Non-Communicating Children's Pain Checklist-Postoperative version (I-NCCPC-PV). METHODS: The original NCCPC-PV version was translated into Italian following the guidelines for "the translation, adaptation, and validation of instruments or scales for cross-cultural healthcare research". We tested the Italian NCCPC-PV version (I-NCCPC-PV) in 40 children (3-18 years of age) with severe to profound Intellectual Disability and no verbal communication. Each child's behavior was observed by a parent or caregiver and by an external observer in a quiet situation and a painful one. They independently assessed the child's level of pain using the translated Italian version of the NCCPCPV (I-NCCPC-PV). RESULTS: The results from 80 assessments showed that children's behavioral signs differed significantly between painful and calm situations (p < 0.001). The inter-rater reliability was poor in a quiet condition (ICC 0.62) and fair in a painful situation (ICC 0.77). The inter-rater agreement was good in both calm and painful conditions (72.50% and 77.50% respectively). CONCLUSION: The Italian version of the NCCPC-PV (I-NCCPC-PV) can be used for pain assessment in children with Intellectual Disability who lack verbal communication

Fyn Mediates Leptin Actions in the Thymus of Rodents

BACKGROUND:Several effects of leptin in the immune system rely on its capacity to modulate cytokine expression and apoptosis in the thymus. Surprisingly, some of these effects are dependent on signal transduction through the IRS1/PI3-kinase, but not on the activation of JAK2. Since all the well known effects of leptin in different cell types and tissues seem to be dependent on JAK2 activation, we hypothesized that, at least for the control of thymic function, another, unknown kinase could mediate the transduction of the leptin signal from the ObR towards the IRS1/PI3-kinase signaling cascade. METHODOLOGY/PRINCIPAL FINDINGS:Here, by employing immunoblot, real-time PCR and flow citometry we show that the tyrosine kinase, Fyn, is constitutively associated with the ObR in thymic cells. Following a leptin stimulus, Fyn undergoes an activating tyrosine phosphorylation and a transient association with IRS1. All these effects are independent of JAK2 activation and, upon Fyn inhibition, the signal transduction towards IRS1/PI3-kinase is abolished. In addition, the inhibition of Fyn significantly modifies the effects of leptin on thymic cytokine expression. CONCLUSION/SIGNIFICANCE:Therefore, in the thymus, Fyn acts as a tyrosine kinase that transduces the leptin signal independently of JAK2 activation, and mediates some of the immunomodulatory effects of leptin in this tissue

Interação entre procedimentos de castração e manejo alimentar na fase de terminação sobre o desempenho e a qualidade do novilho precoce.

Na pecuária de corte a castração é uma prática comum que visa modificar a composição do ganho de peso corporal dos animais. Com o presente estudo objetivou-se avaliar o efeito de diferentes métodos de castração, em pesos e planos nutricionais distintos em novilhos precoces

Semente esverdeada de soja e sua qualidade fisiológica.

Fatores que predispõem a soja à expressão de semente esverdeada; Pigmentos das plantas; Degradação das clorofilas; Efeitos da coloração esverdeada no grão de soja e seus derivados; Efeitos da época da ocorrência de estresses de déficit hídrico e elevadas temperaturas sobre a expressão de semente esverdeada; Efeitos de semente esverdeada sobre a qualidade fisiológica da mesma; Remoção de semente esverdeada na operação de beneficiamento.bitstream/CNPSO-2009-09/25641/1/circular38.pd