36 research outputs found
Mitochondrial Phosphoenolpyruvate Carboxykinase Regulates Metabolic Adaptation and Enables Glucose-Independent Tumor Growth
Elevated Plasma Von Willebrand Factor and Propeptide Levels in Malawian Children with Malaria
In children with malaria plasma VWF and propeptide levels are markedly elevated in both cerebral and mild paediatric malaria, with levels matching disease severity, and these normalize upon recovery. High levels of both markers also occur in retinopathy-negative 'cerebral malaria' cases, many of whom are thought to be suffering from diseases other than malaria, indicating that further studies of these markers will be required to determine their sensitivity and specificity
Large-scale analysis of peptide sequence variants : the case for high-field asymmetric waveform ion mobility spectrometry
[Image: see text] Large scale analysis of proteins by mass spectrometry is becoming increasingly routine; however, the presence of peptide isomers remains a significant challenge for both identification and quantitation in proteomics. Classes of isomers include sequence inversions, structural isomers, and localization variants. In many cases, liquid chromatography is inadequate for separation of peptide isomers. The resulting tandem mass spectra are composite, containing fragments from multiple precursor ions. The benefits of high-field asymmetric waveform ion mobility spectrometry (FAIMS) for proteomics have been demonstrated by a number of groups, but previously work has focused on extending proteome coverage generally. Here, we present a systematic study of the benefits of FAIMS for a key challenge in proteomics, that of peptide isomers. We have applied FAIMS to the analysis of a phosphopeptide library comprising the sequences GPSGXVpSXAQLX(K/R) and SXPFKXpSPLXFG(K/R), where X = ADEFGLSTVY. The library has defined limits enabling us to make valid conclusions regarding FAIMS performance. The library contains numerous sequence inversions and structural isomers. In addition, there are large numbers of theoretical localization variants, allowing false localization rates to be determined. The FAIMS approach is compared with reversed-phase liquid chromatography and strong cation exchange chromatography. The FAIMS approach identified 35% of the peptide library, whereas LC–MS/MS alone identified 8% and LC–MS/MS with strong cation exchange chromatography prefractionation identified 17.3% of the library
Évaluation à l’effort d’une nouvelle méthode de mesure du débit cardiaque basée sur l’analyse de l’onde de pouls (comparaison EsCCO® vs Physioflow®)
International audienc
Recommended from our members
A Randomized Controlled Trial (RCT) of the Effectiveness of Parent Mentors in Improving Asthma Outcomes in Minority Children
HPs (vs. low participants) experienced significantly fewer asthma exacerbations (-20%), missed school (-13%) and parental work days (-20%), and wheezing (-21%) and chest tightness (-14%) episodes, but higher parental satisfaction scores (mean difference=2.85)