21 research outputs found
ISAM 2007 (5th International Symposium of Anaerobic Microbiology) held in Domžale (Slovenia) 21–24 September 2007
Distinct approaches for the detection and removal of chimeric 16S rRNA sequences can significantly affect the outcome of between-site comparisons
Evolutionary relationships and the diversity of the rumen bacteria belonging to the Cytophaga-Flexibacter-Bacteroides phylum
International audienc
A 16S rDNA-based molecular profiling approach for studying relative changes in ruminal bacterial populations
International audienc
Molecular Microbiology of Gut Bacteria: Genetic Diversity and Community Structure Analysis
Recently developed molecular biology approaches make possible the detailed genetic, taxonomic and ecological examination of microorganisms from various habitats. Animal gut represents one of the most complex microbial ecosystems with a large degree of microbial biodiversity present. Bacteria inhabiting the gut usually play important roles in metabolic transformations of substrates and sometimes, e.g. in ruminants, they make the basis for an obligate symbiosis with the host. Here we discuss molecular microbiology as a strategy for examination of gut bacteria, concentrating on a typical and in such environment dominant group of strictly anaerobic Gram-negative bacteria from the phylogenetic group Cytophaga/Flexibacter/Bacteroides. The bacteria from the genus Prevotella are the most abundant Gram-negative bacteria in the rumen and form a distinctive phylogenetic cluster, clearly separated from prevotellas isolated from other ecological niches. They may represent a good choice for a model organism in genetic manipulation experiments and for studies of gene transfer mechanisms taking place in the gut. The molecular tools for detection and monitoring of ruminal prevotellas are discussed