The Spectacular Ionized Interstellar Medium of NGC55

We present deep Halpha+[NII], [SII] (6716,6731A) and [OII] (3726,3729A) images of the highly inclined, actively star--forming SBm galaxy NGC 55, located in the nearby Sculptor Group. Due to its proximity, NGC 55 provides a unique opportunity to study the disk--halo interface in a late--type galaxy with unprecedented spatial resolution. Our images reveal a spectacular variety of ionized gas features, ranging from giant HII region complexes, to supergiant filamentary and shell features, to patches of very faint diffuse emission. Many of these features protrude well above the plane of the galaxy, including a very faint fragmented shell of emission which is visible at 2.6 kpc above the disk. We identify candidate `chimneys' extending out of the disk, which could be the conduits into the halo for hot gas around disk star-forming regions, and could also provide low-density paths for the passage of UV photons from the disk to the halo. Several of the identified chimneys are `capped' with clumps of ionized gas, one of which, located at 1.5 kpc above the disk plane, appears to be the site of recent star formation. Emission--line ratios ([OII]/Halpha+[NII], [SII]/Halpha+[NII]) constrain the ionization mechanism of the gas, and our images allow the first measurement of [OII]/Halpha+[NII] in extra-planar diffuse ionized gas. The diffuse gas is characterized by emission--line ratios which are enhanced on average by a factor of two compared to those of bright HII regions. Each line ratio increases in value smoothly from the cores of HII regions, through the haloes of HII regions, into the diffuse ionized gas. Such a continuous trend is predicted by models in which the diffuse gas is ionized by photons produced by massive stars in HII regions.Comment: 19 pages, including 4 figures, plus 2 external tables. Accepted for publication in the Astronomical Journal. Compressed, postscript versions of the plates are available at ftp://skysrv.pha.jhu.edu/ferguson

The Bipartite Structure of the tRNA m\u3csup\u3e1\u3c/sup\u3eA58 Methyltransferase from \u3cem\u3eS. cerevisiae\u3c/em\u3e is Conserved in Humans

Among all types of RNA, tRNA is unique given that it possesses the largest assortment and abundance of modified nucleosides. The methylation at N1 of adenosine 58 is a conserved modification, occurring in bacterial, archaeal, and eukaryotic tRNAs. In the yeast Saccharomyces cerevisiae, the tRNA 1-methyladenosine 58 (m1A58) methyltransferase (Mtase) is a two-subunit enzyme encoded by the essential genes TRM6 (GCD10) and TRM61 (GCD14). While the significance of many tRNA modifications is poorly understood, methylation of A58 is known to be critical for maintaining the stability of initiator tRNAMet in yeast. Furthermore, all retroviruses utilize m1A58-containing tRNAs to prime reverse transcription, and it has been shown that the presence of m1A58 in human tRNA3 Lys is needed for accurate termination of plus-strand strong-stop DNA synthesis during HIV-1 replication. In this study we have identified the human homologs of the yeast m1A Mtase through amino acid sequence identity and complementation of trm6 and trm61 mutant phenotypes. When coexpressed in yeast, human Trm6p and Trm61p restored the formation of m1A in tRNA, modifying both yeast initiator tRNAMet and human tRNA3 Lys. Stable hTrm6p/hTrm61p complexes purified from yeast maintained tRNA m1A Mtase activity in vitro. The human m1A Mtase complex also exhibited substrate specificity—modifying wild-type yeast tRNAi Met but not an A58U mutant. Therefore, the human tRNA m1A Mtase shares both functional and structural homology with the yeast tRNA m1A Mtase, possessing similar enzymatic activity as well as a conserved binary composition

G. Annette Loller to Mr. Meredith (2 October 1962)

https://egrove.olemiss.edu/mercorr_pro/1459/thumbnail.jp

Pupil needs concept for a junior high school girls' physical education program.

Thesis (Ed.M.)--Boston Universit

Compensating for Missing Data from Longitudinal Studies Using WinBUGS

Missing data is a common problem in survey based research. There are many packages that compensate for missing data but few can easily compensate for missing longitudinal data. WinBUGS compensates for missing data using multiple imputation, and is able to incorporate longitudinal structure using random effects. We demonstrate the superiority of longitudinal imputation over cross-sectional imputation using WinBUGS. We use example data from the Australian Longitudinal Study on Women's Health. We give a SAS macro that uses WinBUGS to analyze longitudinal models with missing covariate date, and demonstrate its use in a longitudinal study of terminal cancer patients and their carers.

Sensory evaluation of Extra Virgin Olive Oil (EVOO) extended to include the quality factor "harmony"

Sensory evaluation of olive oil, meaning the official organoleptic assessment of olive oil respectively the "Panel Test" (PT), is based on the standards of the International Olive Council (IOC), as well as on the Regulation (EC) 640/2008 of the European Commission. These regulations lead to the classification of olive oil as "extra virgin" (EVOO), "virgin" (VOO) or "lampant", which however is not sufficient to clearly discriminate between different quality levels within the grade EVOO. The objective of the study at hand was to develop and validate an objective sensory evaluation method for the quality certification of olive oil within the grade EVOO. A new rating system, including a detailed description and evaluation of the complexity and persistence of flavour, was established. First, a comparison between different profile sheets from various olive oil competitions (Ercole Olivario, Premio Biol, Leone D'Oro Mario Solinas Award, among others) and the official profile sheet from the IOC/EC for the panel test (PT) took place. In consecutive steps the basic test procedure from the panel test (PT) then was extended with additional sensory descriptors. Two trained olive oil panels (the German Olive Oil Panel (DOP) and the Swiss Olive Oil Panel (SOP)) were further educated to profile various green and ripe aroma components and to evaluate the complexity of the perceived aroma components and their persistency (descriptor: "harmony/persistency"). This extended methodology was cross-validated over a time period of 3 years between the two panels (DOP/SOP)

‘Tribes TLC’: Teacher education students’ perceptions

Tribes TLC is a process rather than a programme per se that aims to facilitate interactive learning and assist in the engendering of a positive classroom climate. Devised in the USA and imported into a number of contexts internationally, uptake in Australia has been considerable. Thirty second and third year Bachelor of Education students undertook the training hosted by Cooloongalup Primary School prior to participating in a ten week practicum. Data pertaining to the Tribes TLC resource was collected prior to the training phase, post training, and at the conclusion of the practicum. This paper reports the perceptions of students regarding the nature of Tribes TLC as a resource, their participation in the training, and the value of Tribes training for practicum purposes. Further, insights gleaned regarding the transportability of the resource into other international Teacher Education contexts are discussed

Nuclear Surveillance and Degradation of Hypomodified Initiator tRNA\u3csup\u3eMet\u3c/sup\u3e in \u3cem\u3eS. cerevisiae\u3c/em\u3e

The tRNA m1A58 methyltransferase is composed of two subunits encoded by the essential genes TRM6 and TRM61 (formerly GCD10 and GCD14). The trm6-504 mutation results in a defective m1A methyltransferase (Mtase) and a temperature-sensitive growth phenotype that is attributable to the absence of m1A58 and consequential tRNAiMet instability. We used a genetic approach to identify the genes responsible for tRNAiMet degradation in trm6 cells. Three recessive extragenic mutations that suppress trm6-504 mutant phenotypes and restore hypomodified tRNAiMet to near normal levels were identified. The wild-type allele of one suppressor, DIS3/RRP44, encodes a 3′-5′ exoribonuclease and a member of the multisubunit exosome complex. We provide evidence that a functional nuclear exosome is required for the degradation of tRNAiMet lacking m1A58. A second suppressor gene encodes Trf4p, a DNA polymerase (pol σ) with poly(A) polymerase activity. Whereas deletion of TRF4 leads to stabilization of tRNAiMet, overexpression of Trf4p destabilizes the hypomodified tRNAiMet in trm6 cells. The hypomodified, but not wild-type, pre-tRNAiMet accumulates as a polyadenylated species, whose abundance and length distribution both increase upon Trf4p overexpression. These data indicate that a tRNA surveillance pathway exists in yeast that requires Trf4p and the exosome for polyadenylation and degradation of hypomodified pre-tRNAiMet