8 research outputs found
Elektrik Yaralanmasının Ölümcül Olmayan Bir Komplikasyonu; Katarakt
Yirmi üç yaşında erkek hasta her iki gözünde görme kaybı şikayeti ile kliniğimize
başvurdu. Hastanın alınan öyküsünde 5 yıl önce elektrik akımına
kapıldığı ve bu beş yıl içerisinde görme keskinliğinin giderek azaldığı öğrenildi.
Oftalmolojik muayenede sağ gözde en iyi düzeltilmiş görme keskinliği
6/10 iken sol gözde 2/10 idi. Göz içi basınç her iki gözde normaldi. Biomikroskopik
muayenede iki taraflı ön subkapsüler ve kortikal lens kesafetleri
izleniyordu. Fundus muayenesi her iki gözde normaldi. Hastanın öyküsü ve
göz muayene bulguları ile elektrik kataraktı tanısı kondu. Hastaya topikal
anestezi altında sol göz öncelikli olmak üzere katarakt cerrahisi önerildi.
Cerrahi tedaviyi kabul etmeyen hasta takibe alındı
A Major Public Health Problem: Uncontrolled Wearing of Contact Lenses
Pur po se: To evaluate the behavior, attitude and point of view of contact lens wearers attending Mersin University.
Ma te ri al and Met hod: Our study included students from the Faculty of Medicine whom we assumed to have the highest awareness about
health topics and also students from the Faculty of Education whom we expected to have the similar awareness. Considering the total number
of students, we calculated the minimum number of students attending the Faculty of Education (n: 213) and the minimum number of students
attending the Faculty of Medicine (n: 230) that must be included to the study. Then, we asked the students to answer our questionnaire.
Re sults: The mean age of the students attending the study was 21.29±2.14 years, and 62.5% of the participants were female. Among all
of the students, 81% were wearing contact lenses to correct their refractive errors and 12% of the students were wearing the contact lenses
to enhance appearance for cosmetic reasons. Also, 51% of the students were getting the contact lenses with prescription and the rest of the
students without it. Furthermore, 47.3% of the participants had received the basic information about contact lenses from their doctors, while
the rest had accessed this information through other sources. 43.9% of the participants were visiting their doctors regularly while wearing
contact lenses.
Dis cus si on: Serious complications and irreversible visual loss as a result of this serious complications can occur due to contact lens wear.
So, we think that contact lenses should not be accepted as simple medical devices. (Turk J Ophthalmol 2012; 42: 84-7
Identification of AKT1/β-catenin mutations conferring cetuximab and chemotherapeutic drug resistance in colorectal cancer treatment
In anticancer therapy, the effectiveness of therapeutics is limited by mutations causing drug resistance.KRASmutations are the only determinant for cetuximab resistance in patients with colorectal cancer(CRC). However, cetuximab treatment has not been fully successful in the majority of patients with wild‑type(WT)KRAS. Therefore, it is important to determine new predictive mutations in CRC treatment. In the present study, the association betweenAKT1/β-catenin (CTNNB1) mutations with the drug resistance to cetuximab and other chemotherapeutics used in the CRC treatment was investigated by using site‑directed mutagenesis, transfection, western blotting, and cell proliferation inhibition assay. Cetuximab resistance was higher in the presence ofAKT1E17K, E49K, and L52R mutations, as well asCTNNB1T41A, S45F, and S33P mutations compared with that of respective WT proteins.AKT1/CTNNB1mutations were also associated with oxaliplatin, irinotecan, SN‑38, and 5‑fluorouracil resistance. Furthermore, mutant cell viability in oxaliplatin treatment was more effectively inhibited compared with that of the other chemotherapeutic drugs. In conclusion,AKT1/CTNNB1mutations may be used as an important predictive biomarker in CRC treatment
Antioxidant activities of the methanol and water extracts from Ornithogalum umbellatum
Ornithogalum
(Asparagaceae) species are consumed both as a food and as a therapeutic agent for
their medicinal properties in many countries, including Turkey. The aim of the
study was to investigate the in vitro
antioxidant activities in aerial parts and bulbs of Ornithogalum umbellatum comparatively. The plant materials were
collected from Agva (Istanbul). Methanol and water extracts were prepared
from
the aerial parts and bulbs of the plant. Antioxidant capacities of the extracts
were evaluated by using DPPH radical scavenging activity and ferric reducing
antioxidant power assays along with the determination of total phenolic compounds.
Quercetin was used as a standard antioxidant and total phenolic compounds were
estimated as gallic acid equivalents. The results showed that the methanol extracts
from the aerial parts of O. umbellatum
have the highest total phenolic content and the strongest antioxidant activity.
However, the water extracts from bulbs of the plant did not possess any
antioxidant activity. We can conclude
that, in view of the antioxidant effect of O.
umbellatum, the plant may be a natural source of antioxidant
The cytotoxic effects of Ornithogalum oligophyllum and Ornithogalum umbellatum extracts
Ornithogalum (Asparagaceae) is a genus with
more than 100 species are used in traditionally in many countries, also
consumed in Turkey both as food and for therapeutic purposes. In Turkey, the genus
is represented by 36 species and its regional name is “tükrük otu”. This
study aimed to investigate the cytotoxic effects of Ornithogalum
oligophyllum and Ornithogalum umbellatum. In this context, methanol and water extracts were prepared from the
aerial parts and bulbs of the plants using the Soxhlet apparatus. The cytotoxic
activities of the extracts on prostate (PC-3), lung (A549), endometrium (ECC-1)
cancer cell lines and human umbilical vein/vascular endothelium (HUVEC) control
cell line were determined using [3-(4,5-dimethylthiazol-2-yl)-5-(3-
carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) cell viability
assay. According to the our findings, water extracts of O. oligophyllum
and O. umbellatum had no remarkable cytotoxicity. However, methanol
extracts of O. oligophyllum and O. umbellatum showed the cytotoxic
effect on ECC- 1 cell line. In addition to that, methanol extract of O. umbellatum aerial parts
exhibited cytotoxic activity on PC-3 cancer cell line. In summary, methanolic
extracts from O. oligophyllum and O. umbellatum showed medium cytotoxic activity against the endometrium
and prostate cancer cell line. These findings might be a database for future
studies