İzole kurbağa atriumunda dopamine bağlı muhtemel etkilerin mekanizmasına yönelik bir çalışma

TEZ1549Tez (Uzmanlık) -- Çukurova Üniversitesi, Adana, 1994.Kaynakça (s. 40-42) var.42 s. ; 30 cm.

Akut Gastroenteritli Hastaların Dışkı Örneklerinde Rotavirüs ve Adenovirüs Sıklığı

Aim: The aim of the study was to observe the prevalence of acute gastroenteritis due to rotavirus and adenovirus and analyze annual and seasonal changes. Materials and Methods: The results of the stool samples of the patients with an initial diagnosis of acute gastroenteritis sent to the Microbiology Laboratory from January 2010 to December 2013 were retrospectively analyzed. VIKIA® Rota-Adeno (BioMerieux, France) qualitative Immunochromotographic kit was used to identify of Rotavirus and Adenovirus. SPSS 13.0 for Windows was used for statistical analyses. Chi-square test was used to find relationships between nominal and interval variables. Significance level wa

Pharmacological profile of a nitric oxide donor spermine NONOate in the mouse corpus cavernosum

Background/aim: To investigate the effects of spermine NONOate in the cavernous tissue obtained from mice treated or untreated with sildenafil. Materials and methods: We studied the effects of spermine NONOate on the tone and nitrergic relaxation responses of isolated mouse corpus cavernosum and compared them with sodium nitroprusside in the absence or presence of L-nitroarginine, hydroxocobalamin, pyrogallol, diethyldithiocarbamate (DETCA), or 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). The neurogenic contractions and relaxations of the tissues were induced by electrical field stimulation. Some mice received a single oral dose of sildenafil and after 1 h the effects of spermine NONOate were evaluated by in vitro studies. Results: Spermine NONOate relaxed mouse corpus cavernosum in a concentration-dependent manner. Spermine-NONOate-induced relaxation was relatively slow to develop and it was reversible and reproducible. These relaxations were significantly suppressed by hydroxocobalamin, diethyldithiocarbamate, or ODQ, but not by L-nitroarginine or pyrogallol. Spermine NONOate potentiated the nitrergic relaxations to electrical field stimulation (EFS), whereas it significantly reduced EFS-induced contractions. Sildenafil treatment can enhance the relaxant responses to spermine NONOate and EFS. Conclusion: These findings suggest that spermine NONOate has a potent relaxant action in cavernous tissue and this effect can be potentiated by oral sildenafil treatments. Spermine NONOate may be considered an attractive treatment for erectile dysfunction in pathologic disorders with a lack of endogenous NO production.Background/aim: To investigate the effects of spermine NONOate in the cavernous tissue obtained from mice treated or untreated with sildenafil. Materials and methods: We studied the effects of spermine NONOate on the tone and nitrergic relaxation responses of isolated mouse corpus cavernosum and compared them with sodium nitroprusside in the absence or presence of L-nitroarginine, hydroxocobalamin, pyrogallol, diethyldithiocarbamate (DETCA), or 1H-[1,2,4]Oxadiazolo[4,3-a]quinoxalin-1-one (ODQ). The neurogenic contractions and relaxations of the tissues were induced by electrical field stimulation. Some mice received a single oral dose of sildenafil and after 1 h the effects of spermine NONOate were evaluated by in vitro studies. Results: Spermine NONOate relaxed mouse corpus cavernosum in a concentration-dependent manner. Spermine-NONOate-induced relaxation was relatively slow to develop and it was reversible and reproducible. These relaxations were significantly suppressed by hydroxocobalamin, diethyldithiocarbamate, or ODQ, but not by L-nitroarginine or pyrogallol. Spermine NONOate potentiated the nitrergic relaxations to electrical field stimulation (EFS), whereas it significantly reduced EFS-induced contractions. Sildenafil treatment can enhance the relaxant responses to spermine NONOate and EFS. Conclusion: These findings suggest that spermine NONOate has a potent relaxant action in cavernous tissue and this effect can be potentiated by oral sildenafil treatments. Spermine NONOate may be considered an attractive treatment for erectile dysfunction in pathologic disorders with a lack of endogenous NO production

İzole Kurbağa Akciğer Şeritleri Ve Izoprenalin

İzole Kurbağa Akciğer Şeritleri Ve Izoprenali

Effects of vitamin E and selenium on neurogenic and endothelium-dependent relaxation of corpus cavenosum in the diabetic Mouse

Effects of vitamin E and selenium on neurogenic and endothelium-dependent relaxation of corpus cavernosum in the diabetic Mous

Effects of some divalent cations on the contracture of frog rectus abdominis induced by Na+-free medium at two different temperatures (20C or 30C)

İzole kurbağa rektus abdominis kasında kalsiyumsuz ve sodyumsuz (sukroz ikameti) Ringer solüsyonunun neden olduğu devamlılık arzeden kontraktûrler iki farklı ısı (30°C ve 20°C) ortamında incelendi. Isı farkı kontraktür boyutlarında anlamlı bir değişikliğe neden olmadı. Ca*2, Mn*2 ve Sr*2 doza bağımlı bir tarzda kasılmayı inhibe etti. Banyoya Na* ilave edilmesi ise gelişen kontraktürde konsantrasyona bağımlı gevşemelere neden oldu. İnhibisyon ve gevşeme dereceleri ısı farkından etkilenmedi. Mevcut bulgular sukrozun kalsiyumsuz ve sodyumsuz ortamda, 20°C veya 30°C ısıda fark sergi/emeksizin, muhtemelen çizgili kas membranını etkileyerek kontraktür ile sonuçlanan hücre içi kalsiyum salıverilmesine neden olduğunu ve kalsiyum ile diğer bazı iki değerli katyonların ayrıca tek değerli bir katyon olan sodyumun farklı veya benzer mekanizmaları kullanmak suretiyle oluşan veya oluşmuş olan kontraktürleri inhibe ettiğini telkin etmektedir

Effect of melatonin on contractile activity in intraurethrally instilled E. coli-induced acute prostatitis rat model

Aim: To investigate whether prostatic inflammation affects the contractile responses of prostate tissue and then whether melatonin has any restorative effect on the contractile responses in a rat model of bacterial prostatitis at early stages. Materials and methods: We evaluated the nerve-evoked, adrenergic agonist-induced or cholinergic agonist-induced contractions of isolated prostate tissue at 24 and 72 h after intraurethrally instilled Escherichia coli-treatment. We also analyzed the histological changes in the prostate. Secondarily, we investigated the effect of melatonin pretreatment (7 days, 10 mg kg–1 day–1) on the contractile responses and the histopathologic changes. Results: The degrees of acute inflammatory cell infiltration, acinar changes, and interstitial fibrosis in the prostate glands indicated the development of acute prostatitis at 24 or 72 h after bacterial inoculation. These inflammatory changes were more pronounced at 72 h. Bacterial prostatitis attenuated contractile responses to electrical field stimulation, phenylephrine, or carbachol. The impairment in the contractions was more prominent at 72 h. Melatonin treatment reduced the pathologic changes and partially restored the contractile responses at 72 h of inflammation. Conclusion: Prostatic inflammation caused a diminution in the contractile mechanism of the prostate tissue and melatonin partially restored the contractile responses and histopathologic changes. Melatonin can be useful as an adjuvant to the main therapies for prostatitis to reduce the contractility problems.Aim: To investigate whether prostatic inflammation affects the contractile responses of prostate tissue and then whether melatonin has any restorative effect on the contractile responses in a rat model of bacterial prostatitis at early stages. Materials and methods: We evaluated the nerve-evoked, adrenergic agonist-induced or cholinergic agonist-induced contractions of isolated prostate tissue at 24 and 72 h after intraurethrally instilled Escherichia coli-treatment. We also analyzed the histological changes in the prostate. Secondarily, we investigated the effect of melatonin pretreatment (7 days, 10 mg kg–1 day–1) on the contractile responses and the histopathologic changes. Results: The degrees of acute inflammatory cell infiltration, acinar changes, and interstitial fibrosis in the prostate glands indicated the development of acute prostatitis at 24 or 72 h after bacterial inoculation. These inflammatory changes were more pronounced at 72 h. Bacterial prostatitis attenuated contractile responses to electrical field stimulation, phenylephrine, or carbachol. The impairment in the contractions was more prominent at 72 h. Melatonin treatment reduced the pathologic changes and partially restored the contractile responses at 72 h of inflammation. Conclusion: Prostatic inflammation caused a diminution in the contractile mechanism of the prostate tissue and melatonin partially restored the contractile responses and histopathologic changes. Melatonin can be useful as an adjuvant to the main therapies for prostatitis to reduce the contractility problems