Ideas previas de estudiantes de Odontología respecto al manejo de infecciones odontogénicas en niños

Objetivo: indagar por las ideas previas que presentan los estudiantes de Odontología, que se encuentran cursando entre V a X semestre, respecto al manejo ambulatorio de infecciones odontogénicas –IO- en niños. Métodos: se aplicó un estudio cualitativo-interpretativo con la participación de 60 estudiantes. Las respuestas dadas en una entrevista semiestructurada, la cual contó con ocho preguntas guía abiertas, fueron la unidad de análisis. El procedimiento tuvo cuatro fases. En la primera, preparatoria, se construyó el marco teórico-conceptual como base para interpretar lo manifestado por los estudiantes y se elaboraron las preguntas-guía; para la segunda fase, de recolección de información, se realizaron las entrevistas semiestructuradas presenciales, para ello, los participantes se citaron individualmente en la oficina de investigación. Durante la fase analítica se establecieron las categorías previas: concepto, etiología, evolución, manejo ambulatorio, vías de diseminación, tratamiento farmacológico. La información recabada se vació, depuró y codificó para hacer la exploración con técnica interpretativa y análisis de contenido. En la cuarta fase, de reporte, se presentó un informe con los resultados. Resultados: los estudiantes reconocen que esta enfermedad afecta a pacientes de cualquier edad, sexo y con condición sistemática comprometida. Conclusiones: se requiere una estrategia educativa que permita afianzar el manejo ambulatorio de las IO desde lo propuesto por la ciencia para lograr un aprendizaje significativo. Se requiere enfatizar en el manejo ambulatorio del paciente con infección odontogénica.Objective: To investigate the previous ideas of dental students from the 5th to the 10th semester about the outpatient management of odontogenic infections in children. Methods: Qualitative-interpretive study, with the participation of 60 students. The unit of analysis was the answers given in a semi-structured interview that had eight open-ended questions. The procedure had four phases: Preparatory where the theoretical-conceptual framework was built as a basis for interpreting what was stated by the students, the guiding questions were elaborated. Information gathering: The face-to-face semi-structured interviews were carried out; the participants were cited individually in the research office. Analytical: they were established as previous categories: concept, etiology, evolution, outpatient management, routes of dissemination, pharmacological treatment. The information collected was emptied, refined and encoded, to make the exploration with interpretive technique and content analysis and Report: a report was submitted with the results. Results: The students recognize that this disease affects patients of any age, sex and with a compromised systemic condition. Conclusions: An educational strategy is required that allows to consolidate the outpatient management of IO them from what is proposed by science to achieve meaningful learning. In the case of antibiotic therapy, students are clear about what is done and how long it will last. Emphasis is required on the outpatient management of the patient with odontogenic infection, as long as a good diagnosis is made.https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001237802https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0000824461https://orcid.org/0000-0003-2011-2555https://orcid.org/0000-0002-6299-2135https://scienti.minciencias.gov.co/gruplac/jsp/visualiza/visualizagr.jsp?nro=00000000005594maria.gonzalezbe@campusucc.edu.cohttps://scholar.google.com/citations?user=DakKhIYAAAAJ&hl=es&oi=aohttps://scholar.google.com/citations?user=HyZOE6MAAAAJ&hl=es&oi=a

Ideas previas de estudiantes de Odontología respecto al manejo de infecciones odontogénicas en niños

Objective: To investigate the previous ideas of dental students from the 5th to the 10th semester about the outpatient management of odontogenic infections in children. Methods: Qualitative-interpretive study, with the participation of 60 students. The unit of analysis was the answers given in a semi-structured interview that had eight open-ended questions. The procedure had four phases: Preparatory where the theoretical-conceptual framework was built as a basis for interpreting what was stated by the students, the guiding questions were elaborated. Information gathering: The face-to-face semi-structured interviews were carried out; the participants were cited individually in the research office. Analytical: they were established as previous categories: concept, etiology, evolution, outpatient management, routes of dissemination, pharmacological treatment. The information collected was emptied, refined and encoded, to make the exploration with interpretive technique and content analysis and Report: a report was submitted with the results. Results: The students recognize that this disease affects patients of any age, sex and with a compromised systemic condition. Conclusions: An educational strategy is required that allows to consolidate the outpatient management of IO them from what is proposed by science to achieve meaningful learning. In the case of antibiotic therapy, students are clear about what is done and how long it will last. Emphasis is required on the outpatient management of the patient with odontogenic infection, as long as a good diagnosis is made.Objetivo: indagar por las ideas previas que presentan los estudiantes de Odontología, que se encuentran cursando entre V a X semestre, respecto al manejo ambulatorio de infecciones odontogénicas –IO- en niños. Métodos: se aplicó un estudio cualitativo-interpretativo con la participación de 60 estudiantes. Las respuestas dadas en una entrevista semiestructurada, la cual contó con ocho preguntas guía abiertas, fueron la unidad de análisis. El procedimiento tuvo cuatro fases. En la primera, preparatoria, se construyó el marco teórico-conceptual como base para interpretar lo manifestado por los estudiantes y se elaboraron las preguntas-guía; para la segunda fase, de recolección de información, se realizaron las entrevistas semiestructuradas presenciales, para ello, los participantes se citaron individualmente en la oficina de investigación. Durante la fase analítica se establecieron las categorías previas: concepto, etiología, evolución, manejo ambulatorio, vías de diseminación, tratamiento farmacológico. La información recabada se vació, depuró y codificó para hacer la exploración con técnica interpretativa y análisis de contenido. En la cuarta fase, de reporte, se presentó un informe con los resultados. Resultados: los estudiantes reconocen que esta enfermedad afecta a pacientes de cualquier edad, sexo y con condición sistemática comprometida. Conclusiones: se requiere una estrategia educativa que permita afianzar el manejo ambulatorio de las IO desde lo propuesto por la ciencia para lograr un aprendizaje significativo. Se requiere enfatizar en el manejo ambulatorio del paciente con infección odontogénica

Screening tests for distal symmetrical polyneuropathy in Latin American patients with type 2 diabetes mellitus

ABSTRACT Objective This cross sectional study intended to evaluate two bedside tests (Neuropad and VibraTip) as screening tools for distal symmetrical polyneuropathy (DSPN) in Latin American patients with type 2 diabetes mellitus (T2D). Subjects and methods Ninety-three Colombian patients diagnosed with T2D were recruited. Anthropometric variables, glycemic control parameters, lipid profile and renal function were assessed for each patient. DSPN was defined by a Michigan Neuropathy Screening Instrument (MNSI) clinical score greater than 2. Both Neuropad and Vibratip tests were applied to each patient. Contingency analyses were performed to evaluate the diagnostic power of both tools. Results The prevalence of DSPN determined clinically by MNSI was 25.8%. DSPN in these patients was associated with age, worsening renal function, and insulin treatment. The sensitivity and specificity of the Neuropad test for DSPN was 66.6% and 63% respectively. Its negative predictive value (NPV) was 84.6%. The VibraTip test exhibited a sensitivity of 54.1% and specificity of 91.3%, with a NPV of 85.1%. Conclusion Neuropad and VibraTip are reliable screening tools for DSPN in Latin American population. VibraTip presents a considerable diagnostic power for DSPN in this population. Further studies regarding the cost-effectiveness of these tools in clinical practice are needed

Spatiotemporal regulation of GIPR signaling impacts glucose homeostasis as revealed in studies of a common GIPR variant

Objective: Glucose-dependent insulinotropic polypeptide (GIP) has a role in controlling postprandial metabolic tone. In humans, a GIP receptor (GIPR) variant (Q354, rs1800437) is associated with a lower body mass index (BMI) and increased risk for Type 2 Diabetes. To better understand the impacts of GIPR-Q354 on metabolism, it is necessary to study it in an isogeneic background to the predominant GIPR isoform, E354. To accomplish this objective, we used CRISPR-CAS9 editing to generate mouse models of GIPR-Q354 and GIPR-E354. Here we characterize the metabolic effects of GIPR-Q354 variant in a mouse model (GIPR-Q350). Methods: We generated the GIPR-Q350 mice for in vivo studies of metabolic impact of the variant. We isolated pancreatic islets from GIPR-Q350 mice to study insulin secretion ex vivo. We used a β−cell cell line to understand the impact of the GIPR-Q354 variant on the receptor traffic. Results: We found that female GIPR-Q350 mice are leaner than littermate controls, and male GIPR-Q350 mice are resistant to diet-induced obesity, in line with the association of the variant with reduced BMI in humans. GIPR-Q350 mice of both sexes are more glucose tolerant and exhibit an increased sensitivity to GIP. Postprandial GIP levels are reduced in GIPR-Q350 mice, revealing feedback regulation that balances the increased sensitivity of GIP target tissues to secretion of GIP from intestinal endocrine cells. The increased GIP sensitivity is recapitulated ex vivo during glucose stimulated insulin secretion assays in islets. Generation of cAMP in islets downstream of GIPR activation is not affected by the Q354 substitution. However, post-activation traffic of GIPR-Q354 variant in β-cells is altered, characterized by enhanced intracellular dwell time and increased localization to the Trans-Golgi Network (TGN). Conclusions: Our data link altered intracellular traffic of the GIPR-Q354 variant with GIP control of metabolism. We propose that this change in spatiotemporal signaling underlies the physiologic effects of GIPR-Q350/4 and GIPR-E350/4 in mice and humans. These findings contribute to a more complete understanding of the impact of GIPR-Q354 variant on glucose homeostasis that could perhaps be leveraged to enhance pharmacologic targeting of GIPR for the treatment of metabolic disease

A beta cell subset with enhanced insulin secretion and glucose metabolism is reduced in type 2 diabetes.

The pancreatic islets are composed of discrete hormone-producing cells that orchestrate systemic glucose homeostasis. Here we identify subsets of beta cells using a single-cell transcriptomic approach. One subset of beta cells marked by high CD63 expression is enriched for the expression of mitochondrial metabolism genes and exhibits higher mitochondrial respiration compared with CD63lo beta cells. Human and murine pseudo-islets derived from CD63hi beta cells demonstrate enhanced glucose-stimulated insulin secretion compared with pseudo-islets from CD63lo beta cells. We show that CD63hi beta cells are diminished in mouse models of and in humans with type 2 diabetes. Finally, transplantation of pseudo-islets generated from CD63hi but not CD63lo beta cells into diabetic mice restores glucose homeostasis. These findings suggest that loss of a specific subset of beta cells may lead to diabetes. Strategies to reconstitute or maintain CD63hi beta cells may represent a potential anti-diabetic therapy