19 research outputs found

    Dragging Human Mesenchymal Stem Cells with the Aid of Supramolecular Assemblies of Single-Walled Carbon Nanotubes, Molecular Magnets, and Peptides in a Magnetic Field

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    Human adipose-derived stem cells (hASCs) are an attractive cell source for therapeutic applicability in diverse fields for the repair and regeneration of damaged or malfunctioning tissues and organs. There is a growing number of cell therapies using stem cells due to their characteristics of modulation of immune system and reduction of acute rejection. So a challenge in stem cells therapy is the delivery of cells to the organ of interest, a specific site. The aim of this paper was to investigate the effects of a supramolecular assembly composed of single-walled carbon nanotubes (SWCNT), molecular magnets (lawsone-Co-phenanthroline), and a synthetic peptide (FWYANHYWFHNAFWYANHYWFHNA) in the hASCs cultures. The hASCs were isolated, characterized, expanded, and cultured with the SWCNT supramolecular assembly (SWCNT-MA). The assembly developed did not impair the cell characteristics, viability, or proliferation. During growth, the cells were strongly attached to the assembly and they could be dragged by an applied magnetic field of less than 0.3 T. These assemblies were narrower than their related allotropic forms, that is, multiwalled carbon nanotubes, and they could therefore be used to guide cells through thin blood capillaries within the human body. This strategy seems to be useful as noninvasive and nontoxic stem cells delivery/guidance and tracking during cell therapy

    Interaction between MPN bactericidal proteins and E.coli 0111:B4 coated with complement components.

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    High levels of proinflammatory cytokines such as IFN-γ and TNF are associated with tissue lesions in cutaneous leishmaniasis (CL). We previously demonstrated that Schistosoma mansoni antigens downmodulate the in vitro cytokine response in CL. In the current study we evaluated whether S. mansoni antigens alter monocyte and T-lymphocyte phenotypes in leishmaniasis. Peripheral blood mononuclear cells of CL patients were cultured with L. braziliensis antigen in the presence or absence of the S. mansoni antigens rSm29, rSmTSP-2- and PIII. Cells were stained with fluorochrome conjugated antibodies and analyzed by flow cytometry. The addition of rSm29 to the cultures decreased the expression of HLA-DR in nonclassical (CD14(+)CD16(++)) monocytes, while the addition of PIII diminished the expression of this molecule in classical (CD14(++)CD16(−)) and intermediate (CD14(++)CD16(+)) monocytes. The addition of PIII and rSmTSP-2 resulted in downmodulation of CD80 expression in nonclassical and CD86 expression in intermediate monocytes, respectively. These two antigens increased the expression of CTLA-4 in CD4(+) T cells and they also expanded the frequency of CD4(+)CD25(high)Foxp3(+) T cells. Taken together, we show that S. mansoni antigens, mainly rSmTSP-2 and PIII, are able to decrease the activation status of monocytes and also to upregulate the expression of modulatory molecules in T lymphocytes

    Schistosoma mansoni antigen-driven interleukin-10 production in infected asthmatic individuals

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    Asthmatics infected with Schistosoma mansoni have a less severe course of asthma and an inhibition of the Th2 inflammatory response that seems to be mediated by interleukin (IL-10). The objective of this study was to evaluate the capacity of some S. mansoni antigens to stimulate IL-10 production in vitro by cells of asthmatic infected individuals. Peripheral bloods mononuclear cells were stimulated with the S. mansoni recombinant antigens Sm22.6, Sm14, P24, and PIII antigen. IL-10 was measured in the supernatants of cultures. As the recombinant antigens were cloned in Escherichia coli , we blocked contaminant endotoxin with polymyxin B added to the cultures. We demonstrated that all antigens used drove high production of IL-10 in S. mansoni infected individuals (n = 13, 408 ± 514 and 401 ± 383 pg/ml, 484 ± 245 pg/ml, 579 ± 468 pg/ml, respectively). In asthmatics infected with S. mansoni (n = 21) rP24 induced higher levels of IL-10 (565 ± 377 pg/ml) when compared to PIII, rSm14 and rSm22.6 (184 ± 209 pg/ml; 292 ± 243 pg/ml; 156 ± 247 pg/ml, respectively). Conclusion: the S. mansoni antigens evaluated in this study stimulated IL-10 production by cells from infected individuals and therefore they have the potential to be used as a modulator of the inflammatory response in asthma

    Estudo comparativo da diferenciação osteogĂȘnica das cĂ©lulas tronco mesenquimais da medula Ăłssea e do tecido adiposo de cĂŁes adultos

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    Resumo: O objetivo deste estudo foi comparar o potencial osteogĂȘnico das cĂ©lulas tronco mesenquimais extraĂ­das da medula Ăłssea (CTM-MO) com as do tecido adiposo (CTM-AD) de cĂŁes adultos. As cĂ©lulas foram caracterizadas fenotipicamente quanto Ă  expressĂŁo de CD29, CD90, CD34 e CD45 e submetidas Ă  diferenciação adipogĂȘnica e condrogĂȘnica por 21 dias e osteogĂȘnica por 7, 14 e 21 dias. Foram constituĂ­dos quatro grupos: 1) CTM-MO em meio osteogĂȘnico, 2) CTM-MO em meio basal, 3) CTM-AD em meio osteogĂȘnico e 4) CTM-AD em meio basal. Aos 7, 14 e 21 dias de diferenciação osteogĂȘnica as culturas foram submetidas Ă s avaliaçÔes da conversĂŁo de MTT em formazan, da atividade da fosfatase alcalina (FA), da sĂ­ntese de colĂĄgeno e de matriz mineralizada, avaliação do nĂșmero de cĂ©lulas por campo e foram quantificados os transcritos gĂȘnicos para osterix, sialoproteina Ăłssea (BSP), osteonectina (ON) e osteocalcina (OC). Tanto as cĂ©lulas extraĂ­das da medula Ăłssea quanto do tecido adiposo mostraram elevada expressĂŁo de marcadores para cĂ©lulas tronco e baixa expressĂŁo de marcadores de cĂ©lulas hematopoiĂ©ticas (menor que 2%). AlĂ©m disso, foram capazes de se diferenciar em osteoblastos, condrĂłcitos e adipĂłcitos. As CTM-AD submetidas Ă  diferenciação osteogĂȘnica mostraram maior conversĂŁo do MTT em formazan que as CTM-MO, sob mesmas condiçÔes aos 7 e 21 dias. O nĂșmero de cĂ©lulas por campo, a atividade da FA, a sĂ­ntese de colĂĄgeno e de matriz mineralizada foram superior nas CTM-AD em diferenciação, em relação Ă s CTM-MO sob as mesmas condiçÔes, em todos os tempos estudados. As expressĂ”es de osterix, BSP e OC foram predominantemente superiores nas CTM-MO diferenciadas, mas a expressĂŁo de ON foi superior nas CTM-AD diferenciadas aos 7, 14 e 21 dias. Conclui-se que as CTM-AD apresentam maior potencial osteogĂȘnico que as CTM-MO quando extraĂ­das de cĂŁes adultos
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