A New Evaluation Method of Contact Area at Interface Between Pulsed Surface Discharge and Water

The authors propose a new method for evaluating the contact area of a surface discharge on water by analyzing the resistance of water solution. This method supplements optical observations of light emissions and the Schlieren method. The contact area was modeled as a disk electrode, and the theoretical resistance of water was calculated under the assumption of a mutual similarity between the current and electrostatic fields. A simulated electrostatic field was calculated using a common charge-simulation method. The experimental values of the resistance of water were obtained by dividing the measured voltage drop at water by the measured current. The contact area evaluation was performed by comparing the experimental and theoretical resistance values. The contact area increases with increasing the applied voltage and is practically independent of water depth

Streamer Branching and Spectroscopic Characteristics of Surface Discharge on Water Under Different Pulsed Voltages

Complexity of branching pattern and OH production of water surface discharges was investigated by comparing nanosecond water surface discharge (NWSD) and microsecond water surface discharges (MWSDs) that were defined as NWSD and MWSD, respectively. Experimental and analyzed results between NWSD and MWSD under comparable maximum discharge length l d are summarized as follows: 1) NWSD showed the greatest complexity of branching pattern by fractal analysis; 2) electron density of NWSD was approximately two times greater than MWSD and both orders were 10 -17 cm -3 ; 3) emission intensity of OH (A-X) from MWSD was greater than NWSD; 4) rotational temperature of NWSD was almost constant around 1000 K irrespective of l d and rotational temperature of MWSD increased with increasing l d ranging from 2000 to 4000 K. It was found that the complexity of discharge pattern on water may be affected by the field intensity at water/air boundary. OH production was presumed to be caused by thermal dissociation in this experimental condition

Protection of mice from LPS-induced shock by CD14 antisense oligonucleotide.

CD14 is a pattern recognition receptor on myeloid cells and plays a pivotal role in an innate immune system that is responsible for Gram-negative and Gram-positive bacteria infection. Lipopolysaccharide (LPS), a cell wall component of Gram-negative bacteria, can induce production of a large quantity of proinflammatory cytokines into the circulation mediated by CD14-mediated macrophages and monocytes. These cytokines eventually cause septic shock. Several in vitro and in vivo studies have shown that suppression of a CD14 function by a CD14 antibody led to an inhibition of the production of proinflammatory cytokines such as TNF-alpha, IL-1 beta, and IL-8. In the present study, we found that CD14 antisense oligonucleotide (ODN) can prevent lethal LPS shock in D-galactosamine-sensitized mice. This ODN inhibited CD14 expression in a mouse macrophage cell line, RAW264.7, and suppressed production of TNF-alpha in LPS-stimulated RAW264.7 cells. Furthermore, we designed a consensus antisense ODN that could hybridize human and mouse CD14 RNA, and we evaluated its efficacy. The consensus antisense ODN rescued mice primed with Mycobacterium bovis bacillus Calmette-Guerin (BCG) from the LPS-induced lethal shock. In this model, the CD14 antisense ODN down-regulated LPS-elicited CD14 expression in the liver, resulting in a decrease in LPS-induced TNF-alpha production. These findings suggest that the CD14 antisense ODN is distributed in the liver and efficiently suppresses LPS-induced TNF-alpha production by reducing CD14 expression on Kupffer cells. This CD14 antisense ODN may be useful for the development of a therapeutic agent against sepsis and septic shock.</p

Shock-wave propagation in supercritical CO2 induced by nanosecond-pulsed arc plasma

Shock waves generated by arc plasma in supercritical (SC) CO2 have the potential to create novel reaction fields. However, there have been few studies of shock-wave characteristics in SC-CO2. This study provides the results of visualization of shock-wave propagation in SC-CO2 generated by nanosecond-pulsed arc plasma. A propagating cylindrical shock wave originating from a discharge channel was observed using time-resolved shadowgraph imaging. The shock wave separated from the cylindrical dark-region induced by pulsed arc plasma within 64 nanoseconds. The Mach numbers of the shock waves were investigated against the medium density of the CO2 ranging from a high-pressure gas phase to the SC phase. The Mach number reached a local maximum at the critical CO2 density level. The anomaly in the Mach number can be explained by the local maximum of the specific heat capacity ratio of the CO2 at close to the critical condition of SC-CO2

Neoadjuvant hormonal therapy for low-risk prostate cancer induces biochemical recurrence after radical prostatectomy via increased lymphangiogenesis-related parameters

Background: The effects of neoadjuvant hormonal therapy (NHT) on pathological features and lymphangiogenesis in patients with prostate cancer (PCa) for each pre-operative risk classification are unclear. Methods: To clarify the anti-cancer effects of NHT, we investigated 153 patients (non-NHT group?=?80 and NHT group?=?73) who underwent radical prostatectomy (RP) in Nagasaki University Hospital. Lymph vessel density and area (evaluated by D2-40-positive vessels), vascular endothelial growth factor (VEGF)-C and VEGF-D expressions, and biochemical recurrence (BCR)-free survival were compared between these two groups for each D\u27Amico risk classification (low?=?33, intermediate?=?58, high?=?62 patients). Results: In low-risk PCa patients, the risks of lymph vessel invasion and BCR were significantly higher in the NHT group than in the non-NHT group (P?=?0.040 and 0.022, respectively). Such significant difference was not seen in the intermediate- or high-risk PCa groups. Lymph vessel density of the peri-tumoral and intra-tumoral areas and the lymph vessel area were significantly higher (P?<?0.001) in the NHT group than in the non-NHT group in low-risk PCa. In regard to the expression of VEGF-C or VEGF-D, significant difference was not detected in low-risk PCa. Conclusions: NHT stimulated cancer cell progression and BCR via up-regulation of lymphangiogenesis-related parameters in patients with low-risk PCa. Although VEGF-C and VEGF-D expressions were not changed by NHT, lymph vessel density and area were increased in low-risk PCa patients. We suggest that NHT for patients with low-risk PCa may have a high risk for BCR after RP

Influence of series resistance on dry-band discharge characteristics on wet polluted insulators

This paper describes the current dependence of dry-band discharge. Model electrodes that simulated the dry-band was introduced. The dependence was examined on the voltage and current waveforms, emission spectra and their intensities, rotational temperature, etc. As a result, two different discharge types, continuous and intermittent discharges, were observed in a half cycle of applied ac voltage. They were characterized by continuous current and light emission, and by pulsative current and intermittent light emission, respectively. The duration of the continuous discharge decreased with the value of series resistance, while that of the intermittent discharge increased. The rotational temperatures of the continuous discharge and the intermittent discharge were about 6000 K and 1500 K, respectively. The spectral emission intensity related to the electrolyte during the continuous discharge was higher compared with the intermittent discharge, and decreased with the value of series resistance. The spectral emission intensity related to nitrogen molecule during the intermittent discharge was almost constant irrespective of the value of series resistance. The characteristics of the continuous and intermittent discharges corresponded to those of the terminated partial arc and spark (scintillation), respectively

Underwater shock wave induced by pulsed discharge on water

Plasmas on liquids have provided significant applications in material, environmental, and biological sciences. The mechanisms of these chemical reactions in liquids have been primarily described by the plasma–liquid interactions and convection by an electrohydrodynamic flow. Although shock waves play a significant role in radical formation, agitation and cell destruction, not much information is available on underwater shock waves induced by the surface discharge on water. In this study, an underwater shock wave generated by the pulsed surface discharge on water using the laser shadowgraph method has been demonstrated. The results reveal that the shock wave generated by the discharge on water was transmitted into the water. The mean velocity of the shock wave reached 1.7 km s−1. The results indicate that the surface discharge accelerates the reaction in the water by the combined action of the underwater shock wave and the plasma reaction at the air–water interface. The results are expected to aid in the understanding the mechanisms of existing applications, such as decomposition, synthesis, and sterilization