RT-PCR of EST clones OC2756 (AA0383) and TA0721 showing specific expression in the gonads

EF2, elongation factor 2 gene (control); M, marker lambda-T14I digest; 1, male abdomen; 2, male head; 3, male midgut; 4, testis, 5, 2nd instar nymph; 6, 4th instar nymph, 7, female abdomen; 8, female head; 9, female midgut; 10, ovary of 0-day-old adult; 11, ovary of 4-day-old adult; 12, negative control of no template.<p><b>Copyright information:</b></p><p>Taken from "Annotated ESTs from various tissues of the brown planthopper : A genomic resource for studying agricultural pests"</p><p>http://www.biomedcentral.com/1471-2164/9/117</p><p>BMC Genomics 2008;9():117-117.</p><p>Published online 3 Mar 2008</p><p>PMCID:PMC2311293.</p><p></p

Relation between the number of clusters (vertical) and cluster size (horizontal)

Clusters were calculated using the CLOBB clustering algorithm [25]. The number of clusters (unigenes) was 10,261, with 6,196 singletons.<p><b>Copyright information:</b></p><p>Taken from "Annotated ESTs from various tissues of the brown planthopper : A genomic resource for studying agricultural pests"</p><p>http://www.biomedcentral.com/1471-2164/9/117</p><p>BMC Genomics 2008;9():117-117.</p><p>Published online 3 Mar 2008</p><p>PMCID:PMC2311293.</p><p></p

CDNA nucleotide sequence and putative deduced amino acid sequence of EST clone MB3851

The nucleotide sequence was determined based on the sequence alignment data of cluster members of MB3851. Stop codons are found at the upstream region of the putative first methionine. An InterProScan sequence search [27] showed a signal peptide in the first 18 amino acids (underlined). No homology was found in the DNA and protein databases.<p><b>Copyright information:</b></p><p>Taken from "Annotated ESTs from various tissues of the brown planthopper : A genomic resource for studying agricultural pests"</p><p>http://www.biomedcentral.com/1471-2164/9/117</p><p>BMC Genomics 2008;9():117-117.</p><p>Published online 3 Mar 2008</p><p>PMCID:PMC2311293.</p><p></p

Hybridization of EST clone OC2756 (AA0383) in adult female and TA0721 in adult male

Left, anti-sense probe; right, sense probe. Signals were observed in a part of the lateral oviduct (OC2756) and in an accessory gland of the testis (TA0721).<p><b>Copyright information:</b></p><p>Taken from "Annotated ESTs from various tissues of the brown planthopper : A genomic resource for studying agricultural pests"</p><p>http://www.biomedcentral.com/1471-2164/9/117</p><p>BMC Genomics 2008;9():117-117.</p><p>Published online 3 Mar 2008</p><p>PMCID:PMC2311293.</p><p></p