Role of the Schizosaccharomyces pombe F-box DNA helicase in processing recombination intermediates.

In an effort to identify novel genes involved in recombination repair, we isolated fission yeast Schizosaccharomyces pombe mutants sensitive to methyl methanesulfonate (MMS) and a synthetic lethal with rad2. A gene that complements such mutations was isolated from the S. pombe genomic library, and subsequent analysis identified it as the fbh1 gene encoding the F-box DNA helicase, which is conserved in mammals but not conserved in Saccharomyces cerevisiae. An fbh1 deletion mutant is moderately sensitive to UV, MMS, and ¿ rays. The rhp51 (RAD51 ortholog) mutation is epistatic to fbh1. fbh1 is essential for viability in stationary-phase cells and in the absence of either Srs2 or Rqh1 DNA helicase. In each case, lethality is suppressed by deletion of the recombination gene rhp57. These results suggested that fbh1 acts downstream of rhp51 and rhp57. Following UV irradiation or entry into the stationary phase, nuclear chromosomal domains of the fbh1¿ mutant shrank, and accumulation of some recombination intermediates was suggested by pulsed-field gel electrophoresis. Focus formation of Fbh1 protein was induced by treatment that damages DNA. Thus, the F-box DNA helicase appears to process toxic recombination intermediates, the formation of which is dependent on the function of Rhp51

How patients with cerebrovascular disorder recognize their illness during the acute period

本研究は，急性期における脳血管障害患者が病気体験をどのように認識しているか，を明らかにすることを目的として，看護概念創出法を適用してデータ収集し帰納的に分析を行った．データ収集には，半構成的面接法を用い，研究に同意が得られた成人の脳血管障害患者を対象にインタビューを実施した．その結果，急性期の脳血管障害患者の病気体験に関する認識として，【身体機能と認知の一致プロセス】【思考拡大の限界の気づき】【自分にとっての精神的援助と身体的援助の必要性を実感】【これからの人生に障害を視野に入れて生きることを模索】の４つの概念が創出された．This study investigated how patients with Cerebrovascular disorder become aware of their disability and recognize the illness experience in the acute period. Semi-structured interviews were conducted with three patients with cerebrovascular disorder. Informed consent was obtained from each patient before interviewing. Data were collected using “Kango gainen soushutsuhou （The Method for Creating the Concept of Nursing）” and was analyzed inductively. As a result of this research, four categories were identified as : “Agreement process of body function and recognition”, “Understanding of the limit of their thinking”, “Feeling the necessity for mental and physical care”, “Fumbling through their life history in order to live with their disability”

Prediction of age and brachial-ankle pulse-wave velocity using ultra-wide-field pseudo-color images by deep learning

This study examined whether age and brachial-ankle pulse-wave velocity (baPWV) can be predicted with ultra-wide-field pseudo-color (UWPC) images using deep learning (DL). We examined 170 UWPC images of both eyes of 85 participants (40 men and 45 women, mean age: 57.5 ± 20.9 years). Three types of images were included (total, central, and peripheral) and analyzed by k-fold cross-validation (k = 5) using Visual Geometry Group-16. After bias was eliminated using the generalized linear mixed model, the standard regression coefficients (SRCs) between actual age and baPWV and predicted age and baPWV from the UWPC images by the neural network were calculated, and the prediction accuracies of the DL model for age and baPWV were examined. The SRC between actual age and predicted age by the neural network was 0.833 for all images, 0.818 for central images, and 0.649 for peripheral images (all P < 0.001) and between the actual baPWV and the predicted baPWV was 0.390 for total images, 0.419 for central images, and 0.312 for peripheral images (all P < 0.001). These results show the potential prediction capability of DL for age and vascular aging and could be useful for disease prevention and early treatment