80 research outputs found
Nova Geminorum 1912 and the Origin of the Idea of Gravitational Lensing
Einstein's early calculations of gravitational lensing, contained in a
scratch notebook and dated to the spring of 1912, are reexamined. A hitherto
unknown letter by Einstein suggests that he entertained the idea of explaining
the phenomenon of new stars by gravitational lensing in the fall of 1915 much
more seriously than was previously assumed. A reexamination of the relevant
calculations by Einstein shows that, indeed, at least some of them most likely
date from early October 1915. But in support of earlier historical
interpretation of Einstein's notes, it is argued that the appearance of Nova
Geminorum 1912 (DN Gem) in March 1912 may, in fact, provide a relevant context
and motivation for Einstein's lensing calculations on the occasion of his first
meeting with Erwin Freundlich during a visit in Berlin in April 1912. We also
comment on the significance of Einstein's consideration of gravitational
lensing in the fall of 1915 for the reconstruction of Einstein's final steps in
his path towards general relativity.Comment: 31 p
Viral Oncogene–Induced DNA Damage Response Is Activated in Kaposi Sarcoma Tumorigenesis
Kaposi sarcoma is a tumor consisting of Kaposi sarcoma herpesvirus (KSHV)–infected tumor cells that express endothelial cell (EC) markers and viral genes like v-cyclin, vFLIP, and LANA. Despite a strong link between KSHV infection and certain neoplasms, de novo virus infection of human primary cells does not readily lead to cellular transformation. We have studied the consequences of expression of v-cyclin in primary and immortalized human dermal microvascular ECs. We show that v-cyclin, which is a homolog of cellular D-type cyclins, induces replicative stress in ECs, which leads to senescence and activation of the DNA damage response. We find that antiproliferative checkpoints are activated upon KSHV infection of ECs, and in early-stage but not late-stage lesions of clinical Kaposi sarcoma specimens. These are some of the first results suggesting that DNA damage checkpoint response also functions as an anticancer barrier in virally induced cancers
Semi-automated non-target processing in GC × GC–MS metabolomics analysis: applicability for biomedical studies
Due to the complexity of typical metabolomics samples and the many steps required to obtain quantitative data in GC × GC–MS consisting of deconvolution, peak picking, peak merging, and integration, the unbiased non-target quantification of GC × GC–MS data still poses a major challenge in metabolomics analysis. The feasibility of using commercially available software for non-target processing of GC × GC–MS data was assessed. For this purpose a set of mouse liver samples (24 study samples and five quality control (QC) samples prepared from the study samples) were measured with GC × GC–MS and GC–MS to study the development and progression of insulin resistance, a primary characteristic of diabetes type 2. A total of 170 and 691 peaks were quantified in, respectively, the GC–MS and GC × GC–MS data for all study and QC samples. The quantitative results for the QC samples were compared to assess the quality of semi-automated GC × GC–MS processing compared to targeted GC–MS processing which involved time-consuming manual correction of all wrongly integrated metabolites and was considered as golden standard. The relative standard deviations (RSDs) obtained with GC × GC–MS were somewhat higher than with GC–MS, due to less accurate processing. Still, the biological information in the study samples was preserved and the added value of GC × GC–MS was demonstrated; many additional candidate biomarkers were found with GC × GC–MS compared to GC–MS
The Impact of Dietary Long-Chain Polyunsaturated Fatty Acids on Respiratory Illness in Infants and Children
Fishmeal supplementation during ovine pregnancy and lactation protects against maternal stress-induced programming of the offspring immune system
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