Statistical Analysis Plan: Low Traffic Neighbourhoods in London: Interrupted time series analysis of sensor count data

This document is a statistical analysis plan for the NIHR-funded study into Low Traffic Neighbourhoods in London. It outlines the methodological approach (including data cleaning, anomaly detection, imputation and statistical analysis) associated with automated sensor data collected as part of the project. It gives particular detail to the interrupted time series method that, dependent on data availability and suitability, should form a significant part of the analysis of sensor data

Helicoverpa armigera preference and performance on three cultivars of short-duration pigeonpea (Cajanus cajan): the importance of whole plant assays

BACKGROUND Helicoverpa armigera is a major pest of pigeonpea (Cajanus cajan). Efforts to develop pigeonpea varieties resistant to H. armigera attack have been met with limited success, despite reports of high levels of resistance to H. armigera in wild relatives of pigeonpea and reports of low to moderate levels of resistance in cultivated varieties. Here we examined H. armigera oviposition preference and larval performance on whole plants of three cultivars of short-duration pigeonpea: a susceptible control (ICPL 87) and two cultivars with purported host–plant resistance (ICPL 86012 and ICPL 88039). RESULTS In our no-choice oviposition experiment, H. armigera laid similar numbers of eggs on all three cultivars tested, but under choice conditions moths laid slightly more eggs on ICPL 88039. Larval growth and development were affected by cultivar, and larvae grew to the largest size (weight) and developed fastest on ICPL 86012. Moths laid most of their eggs on floral structures, sites where subsequent early instar larvae overwhelmingly fed. Experimentally placing neonate larvae at different locations on plants demonstrated that larvae placed on flowers experienced greater survival, faster development, and greater weight gain than those placed on leaves. The type and density of trichomes (a potential resistance trait) differed among cultivars and plant structures, but larvae selected to feed at sites where trichomes were absent. CONCLUSION Future work examining host–plant resistance against H. armigera should incorporate the behavioural preference of moths and larvae in experiments using whole plants as opposed to bioassays of excised plant parts in Petri dishes. © 2022 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry

What Defines a Host? Oviposition Behavior and Larval Performance of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Five Putative Host Plants

When an invasive species first breaches quarantine and establishes in yet another country, it invariably causes consternation for growers, in part because of incomplete understanding of the plants that are at risk. The Fall Armyworm, Spodoptera frugiperda (J.E. Smith) is the most recent example in Australia. The number of plants that this polyphagous noctuid is reported to attack is vast, including many crop species. Consequently, initial reactions from grower industry groups that perceived themselves at risk were to demand emergency use of insecticides. Yet the field evidence suggests that many crops might not be at risk and since S. frugiperda arrived in Australia, maize crops have suffered most damage, followed by sorghum. We question the accuracy of some of the claims of reported host plants of S. frugiperda and report experiments that compared oviposition behavior, neonate silking behavior, and larval performance on five crops: the known hosts maize and sorghum, and the putative hosts cotton, peanut, and pigeon pea. Maize ranked highest in all preference and performance measures, followed by sorghum and peanut, with pigeon pea and cotton ranking lowest. Although S. frugiperda can survive, develop, and pupate on the crop species we examined, cotton and pigeon pea are not preferred by the pest in either the larval or adult stages. We suggest that before a plant is listed as a host for a given insect that the evidence should be fully reported and carefully evaluated. Collecting an immature insect from a plant does not make that plant a host

What Defines a Host? Oviposition Behavior and Larval Performance of Spodoptera frugiperda (Lepidoptera: Noctuidae) on Five Putative Host Plants

When an invasive species first breaches quarantine and establishes in yet another country, it invariably causes consternation for growers, in part because of incomplete understanding of the plants that are at risk. The Fall Armyworm, Spodoptera frugiperda (J.E. Smith) is the most recent example in Australia. The number of plants that this polyphagous noctuid is reported to attack is vast, including many crop species. Consequently, initial reactions from grower industry groups that perceived themselves at risk were to demand emergency use of insecticides. Yet the field evidence suggests that many crops might not be at risk and since S. frugiperda arrived in Australia, maize crops have suffered most damage, followed by sorghum. We question the accuracy of some of the claims of reported host plants of S. frugiperda and report experiments that compared oviposition behavior, neonate silking behavior, and larval performance on five crops: the known hosts maize and sorghum, and the putative hosts cotton, peanut, and pigeon pea. Maize ranked highest in all preference and performance measures, followed by sorghum and peanut, with pigeon pea and cotton ranking lowest. Although S. frugiperda can survive, develop, and pupate on the crop species we examined, cotton and pigeon pea are not preferred by the pest in either the larval or adult stages. We suggest that before a plant is listed as a host for a given insect that the evidence should be fully reported and carefully evaluated. Collecting an immature insect from a plant does not make that plant a host

Place-based Politics and Nested Deprivation in the U.K.: Beyond Cities-towns, ‘Two Englands’ and the ‘Left Behind’

‘Place-based explanations’ of politics in the U.K. tell sweeping narratives about ‘Two Englands’, or of sizeable regions of the country that have been ‘Left Behind’, reinforcing popular accounts of a North–South or city-town divide. We introduce the concept of nested deprivation – deprivation that may occur in just one housing estate or even one row of flats within neighbourhoods that are otherwise affluent. We report on intensive fieldwork in 8 neighbourhoods varying in relative affluence and density of population (including urban, suburban/satellite, market town or rural village). Three key themes and consequences emerge for those living in nested deprivation in relatively affluent and geographically dispersed contexts: (a) either disconnection from or entrapment within the local economy; (b) social isolation and atomisation; and (c) powerlessness to affect politics. ‘Place-based’ explanations of rapid and radical changes to political participation in Britain need to take fine-grained geographical distinctions much more seriously. Our study provides evidence that the rising tides in affluent areas are drowning some residents rather than lifting all boats. Where deprivation is dispersed and then nested within mostly affluent constituencies it does not allow for the political mobilisation among communities of interest that is a necessary condition for pluralist representative democracies

Contestable adulthood: variability and disparity in markers for negotiating the transition to adulthood

Recent research has identified a discreet set of subjective markers that are seen as characterizing the transition to adulthood. The current study challenges this coherence by examining the disparity and variability in young people’s selection of such criteria. Four sentence-completion cues corresponding to four differentcontexts in which adult status might be contested were given to 156 British 16- to 17-year-olds. Their qualitative responses were analyzed to explore patterns whilst capturing some of their richness and diversity. An astonishing amount of variability emerged, both within and between cued contexts.The implications of this variability for how the transition to adulthood is experienced are explored. The argument is made that markers of the transition to adulthood are not merely reflective of the bio–psycho–social development of young people. Rather, adulthood here is seen as an essentially contested concept,located within the discursive interactional environment in which young people participate

Climate change and biological control: the consequences of increasing temperatures on host–parasitoid interactions

The relative thermal requirements and tolerances of hymenopteran parasitoids and their hosts were investigated based on published data. The optimal temperature (Topt) for development of parasitoids was significantly lower than that for their hosts. Given the limited plasticity of insect responses to high temperatures and the proximity of Topt to critical thermal maxima, this suggests that host-parasitoid interactions could be negatively affected by increasing global temperatures. A modelling study of the interactions between the diamondback moth and its parasitoid Diadegma semiclausum in Australia indicated that predicted temperature increases will have a greater negative impact on the distribution of the parasitoid than on its host and that they could lead to its exclusion from some agricultural regions where it is currently important

Behavior as a mechanism of insecticide resistance: evaluation of the evidence

'Behavioral resistance' must be defined in a manner consistent with other mechanisms of resistance and be based on heritable changes. Most claimed cases of behavioral resistance to insecticides are simply aversion behaviors either learned or based on simple repellency or avoidance. Although studies have shown changes in taste/odour receptors (e.g., cockroaches), unequivocal demonstration of behavioral resistance to insecticides is rare. The fundamental problems are:1. Inferring resistance from observations, with little evidence of 'normal' behavior prior to exposure to insecticides.2. Interpreting behaviors as insecticide resistance with no evidence that either resistance is detectable or testing the hypothesis that it is responsible. We suggest a way forward that may not be that novel but would advance our understanding and the field