〈Case Reports〉Successful treatment of a necrotizing soft tissue infection with sepsis caused by Aeromonas hydrophila following gastric cancer surgery

A 70-year-old man underwent total gastrectomy and splenectomy for gastric cancer. On the second post-operative day, swelling with redness was noted at the site of drain insertion. The site of redness expanded rapidly and the patient developed purpura with blood blisters complicated by concomitant shock, respiratory failure, and disseminated intravascular coagulation (DIC), leading to a sudden worsening of his general condition. He was diagnosed with a necrotizing soft tissue infection. A drain was inserted through the superficial fascia to initiate irrigation drainage. Aeromonas hydrophila was detected in the subcutaneous effusion and blood cultures. The drainage region was enlarged as needed based on the amount of necrosis. Debridement was performed after his general condition had stabilized. Ninety-two days postoperation, he was transferred to another hospital for skin grafting. Necrotizing soft tissue infections due to A. hydrophila usually have a life-threatening course. However, irrigation drainage through the superficial fascia might be effective when extensive debridement cannot be provided immediately because of a bleeding tendency due to shock or DIC

Ability of bivariate cytokeratin and deoxyribonucleic acid flow cytometry to determine the biologic aggressiveness of resectable non–small cell lung cancer

AbstractObjective: The purpose of this study was to clarify the significance of bivariate cytokeratin and DNA flow cytometry for analysis of the biologic aggressiveness of resectable non-small cell lung cancer. Methods: In 92 patients who underwent curative operations, the DNA ploidy status and S-phase fractions of the cancer cell populations inside the tumors were analyzed by a cytokeratin gating technique with paraffin-embedded specimens and were correlated with the surgical results. Results: Ninety tumors yielded assessable DNA histograms. DNA diploidy was detected in 25 tumors with a mean S-phase fraction of 14.3% ± 4.7%, and DNA aneuploidy was detected in 65 tumors with a mean S-phase fraction of 15.1% ± 7.1%. The 5-year overall and recurrence-free survivals were 73.3% and 70.3%, respectively. Multivariate analysis showed that only TNM staging was a prognostic factor after surgery. There was a negative correlation between the logarithms of S-phase fraction and the disease-free interval for 22 patients with proven recurrence (P =.006). The tumors with high S-phase fractions recurred more rapidly than did those with low S-phase fractions. Conclusion: In a bivariate analysis of cytokeratin and DNA flow cytometry in resectable non-small cell lung cancer, the S-phase fraction appeared to be correlated with the disease-free interval. However, DNA ploidy and S-phase fraction were not predictive of either recurrence or survival after operation. Thus DNA flow cytometry may be of limited use for the analysis of the biologic aggressiveness of lung cancer.J Thorac Cardiovasc Surg 2002;124:293-

The background compensated S phase fraction measurement for cell cycle analysis using flow cytometry : A study on malignant potential in colorectal carcinoma

MultiCycle™ software (Multi-Cycle) is a computer program used to analyze cellular DNA content. This program is useful in mathematically compensating background such as debris, aggregation and sliced nuclei that exist in DNA histograms. Multi-Cycle was used to evaluate S phase fraction (SPF) in colorectal carcinoma cell lines after bromodeoxyuridine labeling. The bromodeoxyuridine labeling index and the SPF were approximate. Also when comparing the same colorectal carcinoma cell lines using Multi-Cycle and a general software, Sum of broadened rectangles model (SOBR), the SPF calculation using Multi-Cycle was significantly lower. To evaluate patient cancers, the SPF was determined using paraffin-embedded materials of colorectal carcinoma. Multi-Cycle showed a low coefficient of variation and SPF. The SOBR was not able to analyze the DNA histogram because of the background. Consequently, Multi-Cycle is indicated as a powerful tool for DNA and cell cycle analysis. When observing colorectal carcinoma from clinicopathological factors, there were no correlations between histological differentiation and DNA ploidy patterns. However, for the clinicopathological factors of invasion beyond the proper muscle, vessel permeation and positive lymph node and liver metastasis, a high DNA aneuploidy was found. Also when comparing the SPF in DNA diploidy and DNA aneuploidy, the proliferative activity of DNA aneuploidy was significantly higher. A high SPF rate was found in DNA aneuploid cancers less than 5.5 cm in size, an invasion beyond the proper muscle and showing positive lymph node metastasis. Furthermore, as the mutual relations between DNA index and SPF have been recognized, DNA aneuploidy can be said to have high malignant potential in the assessment of proliferative activity. Therefore, SPF calculation using Multi-Cycle is recognized as an important indicator in elucidating the malignant potential of colorectal cancer.本文データはCiNiiから複製したものである。departmental bulletin pape