List of genes with expression changes in livers of rats fed an iron-deficient diet.

<p>White arrows: up-regulated gene expression, black arrows: down-regulated gene expression, gray arrows: no change.</p

GO terms associated with the genes that were down-regulated in the iron-deficient group.

<p>FDR-corrected <i>P</i>-values were defined by the modified Fisher's exact test with the Benjamini and Hochberg FDR correction. FDR-corrected <i>P</i>-values<0.05 are shaded in gray.</p

Venn diagrams representing the association of down-regulated genes with multiple GO terms.

<p>The resulting complex interdependencies of categories were shared with differentially expressed genes in the case of non-anemic iron deficiency. The genes are represented as gene symbols.</p

GO terms associated with the genes that were up-regulated in the iron-deficient group.

<p>FDR-corrected <i>P</i>-values were defined by the modified Fisher's exact test with the Benjamini and Hochberg FDR correction. FDR-corrected <i>P</i>-values<0.05 are shaded in gray.</p

Total cholesterol, triacylglycerol and total bile acid levels in the liver and total cholesterol, triacylglycerol, glucose and pyruvate levels in serum.

<p>The values represent the mean ± SEM (n = 5).</p

Expression of <i>G6pc</i> and <i>Hamp</i> in the livers of pair-fed and iron-deficient rats.

<p>Liver sections taken from pair-fed (A, C) and iron-deficient (B, D) rats were analyzed by <i>in situ</i> hybridization using <i>G6pc</i> (A, B) and <i>Hamp</i> (C, D) probes. The data are representative of experiments performed using three rats from each group. Scale bar = 50 µm.</p

Venn diagrams representing the association of up-regulated genes with multiple GO terms.

<p>The resulting complex interdependencies of categories were shared with differentially expressed genes in the case of non-anemic iron deficiency. The genes are represented as gene symbols.</p

Body weight, liver weight, liver iron, hemoglobin, TIBC, serum iron and serum ferritin levels.

<p>The values represent the mean ± SEM (n = 5).</p>*<p><i>P</i><0.05 and</p>**<p><i>P</i><0.01 for between-diet group differences.</p

Diet composition.

1<p>Avicel PH101: sulfite cellulose.</p>2<p>Mineral mix S18706 formulated according to AIN-93 without ferric citrate.</p>3<p>Vitamin mix V10037 formulated according to AIN-93.</p

Potential hepatic metabolic changes due to an iron-deficient diet.

<p>Thick arrows: up-regulated gene expression. Broken arrows: down-regulated gene expression.</p