Analysis of structure and movement of neural growth cone

Growth cone is a sensory and motile part at the tip of a neuron and its main function is path finding. In previous works many chemical cascades and simple structure of growth cone were revealed. However, to understand the whole function of growth cone, deeper research on structure, especially on filopodia, is desired. Here, in this thesis, we developed a new method to segment growth cone and also count and categorize filopodia. Furthermore, evaluation of previous method and this new method were also established. By using this new software, deeper and more detailed research of structure of growth cone is now possible. The method has been tested on real data and evaluated against manually annotated ground truth with promising results

ASCA Observations of OAO 1657-415 and its Dust-Scattered X-Ray Halo

We report on two ASCA observations of the high-mass X-ray binary pulsar OAO 1657-415. A short observation near mid-eclipse caught the source in a low-intensity state, with a weak continuum and iron emission dominated by the 6.4-keV fluorescent line. A later, longer observation found the source in a high-intensity state and covered the uneclipsed through mid-eclipse phases. In the high-intensity state, the non-eclipse spectrum has an absorbed continuum component due to scattering by material near the pulsar and 80 per cent of the fluorescent iron emission comes from less than 19 lt-sec away from the pulsar. We find a dust-scattered X-ray halo whose intensity decays through the eclipse. We use this halo to estimate the distance to the source as 7.1 +/- 1.3 kpc.Comment: Accepted for publication in MNRA

A Search for X-Ray Evidence of a Compact Companion to the Unusual Wolf-Rayet Star HD 50896 (EZ CMa)

We analyze results of a approx.25 ksec ASCA X-ray observation of the unusual Wolf-Rayet star HD 50896 (= EZ CMa). This WN5 star shows optical and ultraviolet variability at a 3.766 day period, which has been interpreted as a possible signature of a compact companion. Our objective was to search for evidence of hard X-rays (greater than or equal to 5 keV) which could be present if the WN5 wind is accreting onto a compact object. The ASCA spectra are dominated by emission below 5 keV and show no significant emission in the harder 5-10 keV range. Weak emission lines are present, and the X-rays arise in an optically thin plasma which spans a range of temperatures from less than or equal to 0.4 keV up to at least approx. 2 keV. Excess X-ray absorption above the interstellar value is present, but the column density is no larger than N(sub H) approx. 10(exp 22)/sq cm. The absorption-corrected X-ray luminosity L(sub x)(0.5 - 10 keV) = 10(exp 32.85) erg/s gives L(sub x)/ L(sub bol) approx. 10(exp -6), a value that is typical of WN stars. No X-ray variability was detected. Our main conclusion is that the X-ray properties of HD 50896 are inconsistent with the behavior expected for wind accretion onto a neutron star or black hole companion. Alternative models based on wind shocks can explain most aspects of the X-ray behavior, and we argue that the hotter plasma near approx. 2 keV could be due to the WR wind shocking onto a normal (nondegenerate) companion

Study of the Long Term Stability of two Anomalous X-ray Pulsars 4U 0142+61 and 1E 1048.1-5937 with ASCA

We present new observations of two anomalous X-ray pulsars (AXP) 4U 0142+61 and 1E 1048.1-5937 made in 1998 with the ASCA. The energy spectra of these two AXPs are found to consist of two components, a power-law and a blackbody emission from the neutron star surface. These observations, when compared to earlier ASCA observations in 1994 show remarkable stability in the intensity, spectral shape and pulse profile. However, we find that the spin-down rate in 1E 1048.1-5937 is not constant. In this source, we have clearly identified three epochs with spin-down rates different from each other and the average value. This has very strong implications for the magnetar hypothesis of AXPs. We also note that the spin-down rate and its variations in 1E 1048.1-5937 are much larger than what can normally be produced by an accretion disk with very low mass accretion rate corresponding to its low X-ray luminosity.Comment: 12 pages, 7 postscript figures included, To appear in the Astrophysical Journa

High resolution X-ray spectrum of the accreting binary X-ray pulsar GX 1+4

We present here high resolution X-ray spectrum of the accreting binary X-ray pulsar GX 1+4 obtained with the High Energy Transmission Grating (HETG) instrument of the Chandra X-ray Observatory. This was supplemented by a simultaneous observation with the Proportional Counter Array (PCA) of the Rossi X-ray Timing Explorer (RXTE). During this observation, the source was in a somewhat low intensity state and the pulse profile with both Chandra and RXTE shows a narrow dip, characteristic of GX 1+4 in medium and low intensity states. The continuum X-ray spectrum obtained with the HETG and PCA can be fitted well with a high energy cutoff power-law model with line of sight absorption. Interestingly, we find that this low state is accompanied by a relatively small absorption column density. A 6.4 keV narrow emission line with an equivalent width of 70 eV is clearly detected in the HETG spectrum. The fluorescence iron line, or at least part of it is produced in the neutral or lowly ionized iron in the circumstellar material that also causes most of the line of sight absorption. In the HETG spectrum, we have found evidence for a weak (equivalent width ~30 eV) emission line at 6.95 keV. This line is identified as Ly_alpha emission line from hydrogen-like iron and the spectrum does not show emission lines from helium-like iron. We discuss various emission regions for the hydrogen-like iron emission line, like gas diffused into the Alfven sphere or an accretion curtain flowing from the inner accretion disk to the magnetic poles.Comment: 15 pages, 4 postscript figures, accepted for publication in The Astrophysical Journa

Quinazolinobenzodiazepine Derivatives, Novobenzomalvins A–C: Fibronectin Expression Regulators from Aspergillus novofumigatus

Three new quinazolinobenzodiazepine derivatives, novobenzomalvins A (1), B (2), and C (3), have been isolated as fibronectin expression regulators from Aspergillus novofumigatus CBS117520. The structures of 1 to 3 were established by spectroscopic and physicochemical analysis, and chemical investigation including the total synthesis of 1. Treatment with novo-benzomalvins A (1), B (2), C (3), and N-methylnovobenzomalvin A (5) increased the expression of fibronectin in normal human neonatal dermal fibroblast cells

X-ray Spectral Study of the Photoionized Stellar Wind in Vela~X-1

We present results from quantitative modeling and spectral analysis of the high mass X-ray binary Vela X-1 obtained with the Chandra HETGS. The spectra exhibit emission lines from H-like and He-like ions driven by photoionization, as well as fluorescent emission lines from several elements in lower charge states. In order to interpret and make full use of the high-quality data, we have developed a simulator, which calculates the ionization and thermal structure of a stellar wind photoionized by an X-ray source, and performs Monte Carlo simulations of X-ray photons propagating through the wind. The emergent spectra are then computed as a function of the viewing angle accurately accounting for photon transport in three dimensions including dynamics. From comparisons of the observed spectra with the simulation results, we are able to find the ionization structure and the geometrical distribution of material in Vela X-1 that can reproduce the observed spectral line intensities and continuum shapes at different orbital phases remarkably well. It is found that a large fraction of X-ray emission lines from highly ionized ions are formed in the region between the neutron star and the companion star. We also find that the fluorescent X-ray lines must be produced in at least three distinct regions --(1)the extended stellar wind, (2)reflection off the stellar photosphere, and (3)in a distribution of dense material partially covering and possibly trailing the neutron star, which may be associated with an accretion wake. Finally, from detailed analysis of the emission lines, we demonstrate that the stellar wind is affected by X-ray photoionization.Comment: 22 pages, 7 tables, 24 figures, accepted for publication in The Astrophysical Journa

Ionization States and Plasma Structures of Mixed-morphology SNRs Observed with ASCA

We present the results of a systematic study using ASCA of the ionization state for six ``mixed-morphology'' supernova emnants (MMSNRs): IC 443, W49B, W28, W44, 3C391, and Kes 27. MMSNRs show centrally filled thermal X-ray emission, which contrasts to shell-like radio morphology, a set of haracteristics at odds with the standard model of SNR evolution (e.g., the Sedov model). We have therefore studied the evolution of the MMSNRs from the ionization conditions inferred from the X-ray spectra, independent of X-ray morphology. We find highly ionized plasmas approaching ionization equilibrium in all the mmsnrs. The degree of ionization is systematically higher than the plasma usually seen in shell-like SNRs. Radial temperature gradients are also observed in five remnants, with cooler plasma toward the limb. In IC 443 and W49B, we find a plasma structure consistent with shell-like SNRs, suggesting that at least some MMSNRs have experienced similar evolution to shell-like SNRs. In addition to the results above, we have discovered an ``overionized'' ionization state in W49B, in addition to that previously found in IC 443. Thermal conduction can cause the hot interior plasma to become overionized by reducing the temperature and density gradients, leading to an interior density increase and temperature decrease. Therefore, we suggest that the ``center-filled'' X-ray morphology develops as the result of thermal conduction, and should arise in all SNRs. This is consistent with the results that MMSNRs are near collisional ionization equilibrium since the conduction timescale is roughly similar to the ionization timescale. Hence, we conclude that MMSNRs are those that have evolved over$\sim10^4$ yr. We call this phase as the ``conduction phase.''Comment: 34 pages, 20 figures, 9 tables, accepted for publication in The Astrophysical Journa

Nature of the Soft Spectral Component in the X-ray Pulsars SMC X-1 and LMC X-4

We present here the results of an investigation of the pulse averaged and pulse phase resolved energy spectra of two high luminosity accretion powered X-ray pulsars SMC X-1 and LMC X-4 made with ASCA. The phase averaged energy spectra definitely show the presence of a soft excess in both the sources. If the soft excess is modeled as a separate black-body or thermal bremsstrahlung type component, pulse phase resolved spectroscopy of SMC X-1 shows that the soft component also has a pulsating nature. Same may be true for LMC X-4, though a very small pulse fraction limits the statistical significance. The pulsating soft component is found to have a nearly sinusoidal profile, dissimilar to the complex profile seen at higher energies, which can be an effect of smearing. Due to very high luminosity of these sources, the size of the emission zone required for the soft component is large (radius ~300-400 km). We show that the pulsating nature of the soft component is difficult to explain if a thermal origin is assumed for it. We further investigated with alternate models, like inversely broken power-law or two different power-law components and found that these models can also be used to explain the excess at low energy. A soft power-law component may be a common feature of the accreting X-ray pulsars, which is difficult to detect because most of the HMXB pulsars are in the Galactic plane and experience large interstellar absorption. In LMC X-4, we have also measured two additonal mid-eclipse times, which confirm the known orbital decay.Comment: 28 pages (AAS preprint), 10 Postscript figures, ApJ accepte