Using the hydrochemical database TANGCHIM to manage groundwater quality data: the case study of a leachate plume from a dumping area

Pollution, landfill, Alpine fluvial valley, TANGCHIM database

Low pressure water vapour plasma treatment of surfaces for biomolecules decontamination.

Abstract Decontamination treatments of surfaces are performed on bacterial spores, albumin and brain homogenate used as models of biological contaminations in a low-pressure, inductively coupled plasma reactor operated with water-vapour-based gas mixtures. It is shown that removal of contamination can be achieved using pure H2O or Ar/H2O mixtures at low temperatures with removal rates comparable to oxygen-based mixtures. Particle fluxes (Ar+ ions, O and H atomic radicals and OH molecular radicals) from water vapour discharge are measured by optical emission spectroscopy and Langmuir probe under several operating conditions. Analysis of particle fluxes and removal rates measurements illustrates the role of ion bombardment associated with O radicals, governing the removal rates of organic matter. Auxiliary role of hydroxyl radicals is discussed on the basis of experimental data. The advantages of a water vapour plasma process are discussed for practical applications in medical devices decontamination.</jats:p

Banca dati idrogeologica TANGRAM©: strumento per elaborazioni quantitative di dati per la valutazione delle acque sotterranee - The hydrogeological well database TANGRAM©: a tool for data processing to support groundwater assessment

At the Department of Earth and Environmental Sciences of the University of Milano-Bicocca (DISAT-UNIMIB), a hydrogeological well database, called TANGRAM©, has been developed and published on line at www.TANGRAM.samit.unimib.it, developing an earlier 1989 DOS version. This package can be used to store, display, and process all data related to water wells, including administrative information, well characteristics, stratigraphic logs, water levels, pumping rates, and other hydrogeological information. Currently, the database contains more than 39.200 wells located in the Italian region of Lombardy (90%), Piedmont (9%) and Valle d'Aosta (1%). TANGRAM© has been created both as a tool for researches and for public administration's administrators who have projects in common with DISAT-UNIMIB. Indeed, transferring wells data from paper into TANGRAM© offers both an easier and more robust way to correlate hydrogeological data and a more organized management of the administrative information. Some Administrations use TANGRAM© regularly as a tool for wells data management (Brescia Province, ARPA Valle Aosta). An innovative aspect of the database is the quantitative extraction of stratigraphic data. In the part of the software intended for research purposes, all well logs are translated into 8-digit alphanumeric codes and the user composes the code interpreting the description at each stratigraphic level. So the stratigraphic well data can be coded, then quantified and processed. This is made possible by attributing a weight to the digits of the code for textures. The program calculates the weighted percentage of the chosen lithology, as related to each individual layer. These extractions are the starting point for subsequent hydrogeological studies: well head protection area, reconstruction of the dynamics of flow, realization of the quarry plans and flux and transport hydrogeological models. The results of a two-dimensional distribution of coarse, medium and fine sized material in the first 80 meters of depth are presented here for a study area located within the Province of Brescia (Italy)

How to manage potential groundwater contaminations by As, Fe and Mn in lower Po Plain: a proposal from the case study of Cremona

The Legislative Decree 152/06 on environmental regulations also governs polluted site remediation (Part IV). A potentially contaminated site is defined when concentrations exceed the limits reported in the Attachment 5 (Title V). Nevertheless, the Legislative Decree 152/06 considers the possibility of higher limits than Attachment 5 for trace elements in the case of natural high contents by the definition of natural background levels. The lower Po Plain is characterized by high natural concentrations of As, Fe and Mn in groundwater and, at the same time, is one of the most important areas for the Italian productive system, and thus, possible direct sources of As, Fe and Mn or indirect anthropogenic influences could also exist. Therefore, an operative tool is required to determine the natural background levels and to understand if measured high concentrations are attributable to natural backgrounds or to anthropogenic sources/influences, and consequently, to decide on the beginning of the administrative procedure requested by the Legislative Decree 152/09 through the site characterization phase. This work presents a proposal of procedure to manage potentially contaminated site by As, Fe and Mn in the lower Po Plain, that was developed in the framework of a scientific collaboration between the Province of Cremona and the University of Milano-Bicocca. The procedure involves the following four steps, preceded by the derivation of natural background levels as step zero: (1) comparing new measurements to natural background levels; (2) testing the presence of direct anthropogenic sources of As, Fe and Mn; (3) excluding false positives and (4) testing the presence of indirect anthropogenic influences on As, Fe and Mn concentrations. The operative application of the proposed procedure is actually under consideration by the Province of Cremona

Preliminary groundwater modelling by considering the interaction with superficial water: Aosta plain case (northern Italy)

The study is developed through scientific cooperation between the University of Milano-Bicocca and the Regional Agency for Environmental Protection (ARPA) of the Valle d'Aosta Region. Its aim is to produce a decision-support tool to help the Public Administration'manage groundwater and public water supply. The study area is the plain of Aosta, between the cities of Aymavilles and Brissogne; in this area groundwater represents the main source of public water supply. The valley is oriented east-west, along the Baltea for a length of 13.1 km and a width of 4.6 km. The textural and hydrogeological properties of the deposits are strictly connected to glacial deposition and to the subsequent sedimentary processes which took place in glacial, lacustrine and fluvial systems. The study is based on available well information in the Aosta plain - including water wells (133) and piezometers (121) - which have been coded and stored in the well database TANGRAM,. The database facilitates interpretation of the well data, and it allows three-dimensional mapping of subsurface hydrogeological characteristics through database codification and ordinary kriging interpolation. The study is designed to achieve two objectives. The first is to provide the Aosta Public Authorities with a well database in order to simplify groundwater management. The second is to provide Public Authorities with a groundwater flow model of the local aquifer. The model integrates surface and subsurface flows in order to fully account for all important stresses, both natural and anthropogenic, on the groundwater system. It provides a tool for testing hypotheses (such as the impact of new wells) and thereby allows science-based management of the aquifer resource

G PROTEIN-COUPLED RECEPTOR DESENSITISATION REGULATES STEM CELL DIFFERENTIATION

G-protein coupled receptors (GPCRs) play a key role in many complex biological processes, including regulation of stem cell pluripotency and differentiation. Signal transduction pathways that are activated during stem cell renewal and differentiation are shared, cross-activated or synergistic with GPCR stimulation [1]. Regulation of GPCR responses involved the activation of desensitization machinery, which started with phosphorylation of agonist-activated receptor by second messenger-dependent and/or GPCR kinases (GRKs)[1]. Besides controlling receptor responsiveness, GRKs can also act as agonist-regulated scaffolds assembling macromolecular signalosomes in the receptor environment, thereby contributing to signal propagation from cytosol to nucleus, and controlling gene transcription machinery [2]. Recent evidence suggests that the desensitization machinery fulfils a vital role in regulating cellular responses to GPCRs, and that changes in expression/functioning of these regulatory proteins may be crucial in the control of cell differentiation program [3]. These data are consistent with the notion that GPCR responsiveness may be differentially regulated during cell differentiation. In our hands, two different cellular models (oligodendrocyte precursor cells, OPCs, and mesenchymal stem cells, MSCs) were used to investigate the role of the GPCR desensitisation machinery in stem cell differentiation. During OPC differentiation, defective control of the membrane receptor GPR17 has been suggested to block cell maturation and impairs remyelination under demyelinating conditions [4]. Here we show, for the first time, a role for Murine double minute 2 (Mdm2), a ligase previously involved in ubiquitination/degradation of p53 protein. In maturing OPCs, the inhibition of Mdm2-p53 interactions increased GRK2 sequestration by Mdm2, leading to impaired GPR17 down-regulation and OPC maturation block. In MSCs, the A2B adenosine receptor (A2BAR) has been recently emerged as the major AR involved in osteoblastogenesis [5]. Proinflammatory cytokines, such as Tumour Necrosis Factor- (TNF-, have been demonstrated to regulate MSC differentiation and bone remodelling. Herein, we show that TNF- diminished GRK2 levels in MSCs, thus blocking A2BAR desensitization. As a result, TNF- enhanced the A2BAR-mediated responses and favoured MSC differentiation to osteoblasts in response to receptor agonists. The findings get new insights for discovering of the signals at the basis of cell differentiation

Hydrogeological conceptual model of a highly impacted watershed: the case study of Oglio river (n Italy)

Oglio River watershed, Italy, water bodies, TANGRAM, groundwater discharge

Promotion of manual drilling in Guinea Bissau: mapping suitable zones and estimating the potential

Guinea Bissau has made large strides in water supply, but still 35% of villages have no access to safe water. UNICEF is promoting alternative solutions to ensure a proper water supply in these small communities, for example manual drilling. For these reason a mapping study to identify suitable zones for these techniques has been carried out in 2016, using existing groundwater data and the experience from local experts. 88% of Guinea Bissau is considered with feasible hydrologeogical conditions for manual drilling. In 63% of the regions where this technique is applicable, it is adequate for villages of small-medium size. Taking into consideration the results of this study, it seems evident that the promotion of manual drilling could be a valid alternative to improve the situation of access to safe water especially in rural areas

The ubiquitin ligase Mdm2 controls oligodendrocyte maturation by intertwining mTOR with G protein-coupled receptor kinase 2 in the regulation of GPR17 receptor desensitization

During oligodendrocyte precursor cell (OPC) differentiation, defective control of the membrane receptor GPR17 has been suggested to block cell maturation and impair remyelination under demyelinating conditions. After the immature oligodendrocyte stage, to enable cells to complete maturation, GPR17 is physiologically down-regulated via phosphorylation/desensitization by G protein-coupled receptor kinases (GRKs); conversely, GRKs are regulated by the "mammalian target of rapamycin" mTOR. However, how GRKs and mTOR are connected to each other in modulating GPR17 function and oligodendrogenesis has remained elusive. Here we show, for the first time, a role for Murine double minute 2 (Mdm2), a ligase previously involved in ubiquitination/degradation of the onco-suppressor p53 protein. In maturing OPCs, both rapamycin and Nutlin-3, a small molecule inhibitor of Mdm2-p53 interactions, increased GRK2 sequestration by Mdm2, leading to impaired GPR17 down-regulation and OPC maturation block. Thus, Mdm2 intertwines mTOR with GRK2 in regulating GPR17 and oligodendrogenesis and represents a novel actor in myelination