Supplanting crystallography or supplementing microscopy? A combined approach to the study of an enveloped virus

The recent advances in the resolution obtained by single-particle reconstructions from cryo-electron microscopy (cryo-EM) have led to an increase in studies that combine X-ray crystallographic results with those of electron microscopy (EM). Here, such a combination is described in the determination of the structure of an enveloped animal virus, Semliki Forest virus, at 9 Å resolution. The issues of model bias in determination of the structure, the definition of resolution in a single-particle reconstruction, the effect of the correction of the contrast-transfer function on the structure determined and the use of a high-resolution structure of a subunit in the interpretation of the structure of the complex are addressed

Artificial atmosphere control system

Two-gas control system has been developed which uses existing hardware. Three systems are used for control, monitoring, and safety backup. Pure oxygen will be supplied to maintain safe pressure level should something go wrong

An experimental investigation of the interior noise control effects of propeller synchrophasing

A simplified cylindrical model of an aircraft fuselage is used to investigate the mechanisms of interior noise suppression using synchrophasing techniques. This investigation allows isolation of important parameters to define the characteristics of synchrophasing. The optimum synchrophase angle for maximum noise reduction is found for several interior microphone positions with pure tone source excitation. Noise reductions of up to 30 dB are shown for some microphone positions, however, overall reductions are less. A computer algorithm is developed to decompose the cylinder vibration into modal components over a wide range of synchrophase angles. The circumferential modal response of the shell vibration is shown to govern the transmission of sound into the cylinder rather than localized transmission. As well as investigating synchrophasing, the interior sound field due to sources typical of propellers has been measured and discussed

Numerical simulations of the seasonal/latitudinal variations of atomic oxygen and nitric oxide in the lower thermosphere and mesosphere

A 2-Dimensional zonally-averaged thermospheric model and the global University College London (UCL) thermospheric model have been used to investigate the seasonal, solar activity and geomagnetic variation of atomic oxygen and nitric oxide. The 2-dimensional model includes detailed oxygen and nitrogen chemistry, with appropriate completion of the energy equation, by adding the thermal infrared cooling by O and NO. This solution includes solar and auroral production of odd nitrogen compounds and metastable species. This model has been used for three investigations; firstly, to study the interactions between atmospheric dynamics and minor species transport and density; secondly, to examine the seasonal variations of atomic oxygen and nitric oxide within the upper mesosphere and thermosphere and their response to solar and geomagnetic activity variations; thirdly, to study the factor of 7 to 8 peak nitric oxide density increase as solar F sub 10.7 cm flux increases from 70 to 240 reported from the Solar Mesospheric Explorer. Auroral production of NO is shown to be the dominant source at high latitudes, generating peak NO densities a factor of 10 greater than typical number densities at low latitudes. At low latitudes, the predicted variation of the peak NO density, near 110 km, with the solar F sub 10.7 cm flux is rather smaller than is observed. This is most likely due to an overestimate of the soft X-ray flux at low solar activity, for times of extremely low support number, as occurred in June 1986. As observed on pressure levels, the variation of O density is small. The global circulation during solstice and periods of elevated geomagnetic activity causes depletion of O in regions of upwelling, and enhancements in regions of downwelling

The art of being human : a project for general philosophy of science

Throughout the medieval and modern periods, in various sacred and secular guises, the unification of all forms of knowledge under the rubric of ‘science’ has been taken as the prerogative of humanity as a species. However, as our sense of species privilege has been called increasingly into question, so too has the very salience of ‘humanity’ and ‘science’ as general categories, let alone ones that might bear some essential relationship to each other. After showing how the ascendant Stanford School in the philosophy of science has contributed to this joint demystification of ‘humanity’ and ‘science’, I proceed on a more positive note to a conceptual framework for making sense of science as the art of being human. My understanding of ‘science’ is indebted to the red thread that runs from Christian theology through the Scientific Revolution and Enlightenment to the Humboldtian revival of the university as the site for the synthesis of knowledge as the culmination of self-development. Especially salient to this idea is science‘s epistemic capacity to manage modality (i.e. to determine the conditions under which possibilities can be actualised) and its political capacity to organize humanity into projects of universal concern. However, the challenge facing such an ideal in the twentyfirst century is that the predicate ‘human’ may be projected in three quite distinct ways, governed by what I call ‘ecological’, ‘biomedical’ and ‘cybernetic’ interests. Which one of these future humanities would claim today’s humans as proper ancestors and could these futures co-habit the same world thus become two important questions that general philosophy of science will need to address in the coming years

The genealogy of judgement: towards a deep history of academic freedom

The classical conception of academic freedom associated with Wilhelm von Humboldt and the rise of the modern university has a quite specific cultural foundation that centres on the controversial mental faculty of 'judgement'. This article traces the roots of 'judgement' back to the Protestant Reformation, through its heyday as the signature feature of German idealism, and to its gradual loss of salience as both a philosophical and a psychological concept. This trajectory has been accompanied by a general shrinking in the scope of academic freedom from the promulgation of world-views to the offering of expert opinion

Shearing or Compressing a Soft Glass in 2D: Time-concentration superposition

We report surface shear rheological measurements on dense insoluble monolayers of micron sized colloidal spheres at the oil/water interface and of the protein $\beta$-lactoglobulin at the air/water surface. As expected, the elastic modulus shows a changing character in the response, from a viscous liquid towards an elastic solid as the concentration is increased, and a change from elastic to viscous as the shear frequency is increased. Surprisingly, above a critical packing fraction, the complex elastic modulus curves measured at different concentrations can be superposed to form a master curve, by rescaling the frequency and the magnitude of the modulus. This provides a powerful tool for the extrapolation of the material response function outside the experimentally accessible frequency range. The results are discussed in relation to recent experiments on bulk systems, and indicate that these two dimensional monolayers should be regarded as being close to a soft glass state.Comment: to appear in PR

A regional case in the development of agriculture and crop-processing in northern China from the Neolithic to Bronze Age: archaeobotanical evidence from the Sushui River survey, Shanxi Province

The article presents the results of the analysis of survey archaeobotany samples from the Sushui valley. This provides evidence for changes over time for a region in the proportions of crops, especially rice versus millets. In addition, the composition of samples, both grouped by period and considered on a sample-by-sample basis, are considered as representing routine crop-processing waste, from which it is suggested that typical patterns of routine crop processing (after storage) can be inferred. These patterns, in turn, imply something about processing prior to storage and the social organization of agricultural production, suggesting the hypothesis that crop-processing patterns diversified during the emergence of complex societies with some sites with larger scale practices while others were focused on the household level

Twist and writhe dynamics of stiff filaments

This letter considers the dynamics of a stiff filament, in particular the coupling of twist and bend via writhe. The time dependence of the writhe of a filament is $W_r^2\sim L t^{1/4}$ for a linear filament and $W_r^2\sim t^{1/2} / L$ for a curved filament. Simulations are used to study the relative importance of crankshaft motion and tube like motion in twist dynamics. Fuller's theorem, and its relation with the Berry phase, is reconsidered for open filamentsComment: 7 Pages with 2 figure

Satisfaction Among Ecological Management Workers

This paper explores levels and correlates of job satisfaction for a series of occupations concerned with ecological management in Virginia. To enhance job satisfaction, a careful balance must be maintained between organizational requirements and individual needs. Fortunately, organizational factors over which the manager can exert considerable control (specially, dimensions of bureaucratization) are more consistently related to job satisfaction than are individual factors (extent of job training, evaluation of job training, education, and job tenure)