Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae

Strains of Pseudomonas syringae pv. syringae resistant to copper, streptomycin, or both compounds were recovered from symptomless and diseased tissue of four woody hosts in three nurseries in Oklahoma. In strains resistant to copper and streptomycin (Cu^r Sm^r), resistance to both compounds was cotransferred with a single plasmid which was either 68, 190, or 220 kilobase pairs (kb). All Cu^s Sm^r strains contained a 68-kb conjugative plasmid. Cu^r Sm^s, strains contained one plasmid which varied in size from 60 to 73 kb. All conjugative plasmids which transferred streptomycin resistance contained sequences homologous to the strA and strB Sm^r genes from the broad-host-range plasmid RSF1010. The Sm^r determinant was subsequently cloned from a 68-kb Cu^r Sm^r plasmid designated pPSR1. A restriction map detailing the organization of the homologous Sm^r genes from pPSR1 and RSF1010 and cloned Sm^r genes from P. syringae pv. papulans and Xanthomonas campestris pv. vesicatoria revealed the conservation of all sites studied. The Cu^r genes cloned from P. syringae pv. tomato PT23 and X. campestris pv. vesicatoria XV10 did not hybridize to the Cu^r plasmids identified in the present study, indicating that copper resistance in these P. syringae pv. syringae strains may be conferred by a distinct genetic determinant.Peer reviewedPlant Patholog

3C 40 in Abell 194: can tail radio galaxies exist in quiescent cluster

The definitive version is available at www.blackwell-synergy.com. Copyright Blackwell Publishing DOI : 10.1111/j.1365-2966.2007.12465.xThe nearby cluster Abell 194 hosts two luminous, distorted radio galaxies. Both reside within the cluster’s core region, being separated in projection by only 100 kpc. It is often suggested that tailed radio galaxies such as these reside in clusters that are under formation and are accreting new material from their outskirts. In this paper we study the intriguing appearance of Abell 194, and test whether the cluster and radio source dynamics are consistent with the cluster formation/merger model. We analyse data from the XMM-Newton satellite and previously unpublished observations with the Very Large Array (VLA), as well as presenting new data from the Giant Metre-Wave Radio Telescope (GMRT). The shape of the jets, and the lack of significant stripping of the galaxies’ interstellar media, indicate that the radio galaxies are not moving at the large velocities they would have had if they were falling into the cluster from its outskirts; galaxy velocities of 6 300 km s−1 are adequate instead. A plausible scenario that could explain the observations is that the dynamics of the cluster centre are relatively quiescent, with the dominant system of massive galaxies being bound and orbiting the cluster centre of mass. For plausible jet/plume speeds and densities and the galaxy dynamics implied by this picture of the cluster, we show that the observed jet structures can be explained without invoking a major cluster merger event.Peer reviewe

Eustatic signals in deep-marine sedimentary sequences recovered at ODP Site 978, Alboran Basin, western Mediterranean Sea

A continuous section of Pliocene marine sediments was recovered at Ocean Drilling Program Site 978, located in the Alboran Sea between Spain and Morocco. Three Pliocene subunits have been defined at Site 978: the lowermost (Subunit IC, 129.2 m thick) is characterized by alternating beds of lighter, more calcareous, and darker less calcareous, claystone with bioturbated upper and lower contacts (Type 1 cycles); the middle (Subunit 1B, 67.1 m thick) is composed of relatively homogeneous nannofossil claystone; and the uppermost (Subunit IA, 211.6 m thick) contains abrupt-based darker, terrigenous layers interpreted as turbidites that are interstratified with lighter nannofossil claystone (Type 2 cycles). The rhythmically bedded light and dark layers in Subunit IC correlate with those in the Rosello Composite Section of Sicily, a global reference standard for the Pliocene time scale. These sedimentary cycles are products of variations in precession and resulting continental runoff. Missing cycles occur during eustatic highstands. The shift to more homogeneous sedimentation in Subunit IB is represented in similar-aged sequences throughout the Mediterranean which display evidence of submarine mass wasting. Mediterranean-wide slope degradation was likely a response to rapid sea-level change at approximately 3 Ma. This change in sedimentation style was accompanied by an upsection increase in sediment accumulation rates associated with turbidite influx in Subunit IA. Turbidite frequency throughout the Pliocene section can be linked to eustatic changes in sea level, with turbidite maxima corresponding with mid-sequence downlap surfaces and their associated condensed sections. © 2004, SEPM (Society for Sedimentary Geology)