Coupling of ultrathin tapered fibers with high-Q microsphere resonators at cryogenic temperatures and observation of phase-shift transition from undercoupling to overcoupling

We cooled ultrathin tapered fibers to cryogenic temperatures and controllably coupled them with high-Q microsphere resonators at a wavelength close to the optical transition of diamond nitrogen vacancy centers. The 310-nm-diameter tapered fibers were stably nanopositioned close to the microspheres with a positioning stability of approximately 10 nm over a temperature range of 7-28 K. A cavity-induced phase shift was observed in this temperature range, demonstrating a discrete transition from undercoupling to overcoupling

Catalogue of epidermal genes: Genes expressed in the epidermis during larval molt of the silkworm Bombyx mori

<p>Abstract</p> <p>Background</p> <p>The insect cuticle is composed of various proteins and formed during the molt under hormonal regulation, although its precise composition and formation mechanism are largely unknown. The exhaustive catalogue of genes expressed in epidermis at the molt constitutes a massive amount of information from which to draw a complete picture of the molt and cuticle formation in insects. Therefore, we have catalogued a library of full-length cDNAs (designated epM) from epidermal cells during the last larval molt of <it>Bombyx mori</it>.</p> <p>Results</p> <p>Of the 10,368 sequences in the library, we isolated 6,653 usable expressed sequence tags (ESTs), which were categorized into 1,451 nonredundant gene clusters. Seventy-one clusters were considered to be isoforms or premature forms of other clusters. Therefore, we have identified 1,380 putative genes. Of the 6,653 expressed sequences, 48% were derived from 92 cuticular protein genes (RR-1, 24; RR-2, 17; glycine-rich, 29; other classes, 22). A comparison of epM with another epidermal EST data set, epV3 (feeding stage: fifth instar, day 3), showed marked differences in cuticular protein gene. Various types of cuticular proteins are expressed in epM but virtually only RR-1 proteins were expressed in epV3. Cuticular protein genes expressed specifically in epidermis, with several types of expression patterns during the molt, suggest different types of responses to the ecdysteroid pulse. Compared with other <it>Bombyx </it>EST libraries, 13 genes were preferentially included in epM data set. We isolated 290 genes for proteins other than cuticular proteins, whose amino acid sequences retain putative signal peptides, suggesting that they play some role in cuticle formation or in other molting events. Several gene groups were also included in this data set: hormone metabolism, P450, modifier of cuticular protein structure, small-ligand-binding protein, transcription factor, and pigmentation genes.</p> <p>Conclusion</p> <p>We have identified 1,380 genes in epM data set and 13 preferentially expressed genes in epidermis at the molt. The comparison of the epM and other EST libraries clarified the totally different gene expression patterns in epidermis between the molting and feeding stages and many novel tissue- and stage-specifically expressed epidermal genes. These data should further our understanding of cuticle formation and the insect molt.</p

Saari's homographic conjecture for planar equal-mass three-body problem under a strong force potential

Donald Saari conjectured that the $N$-body motion with constant configurational measure is a motion with fixed shape. Here, the configurational measure $\mu$ is a scale invariant product of the moment of inertia $I=\sum_k m_k |q_k|^2$ and the potential function $U=\sum_{i<j} m_i m_j/|q_i-q_j|^\alpha$, $\alpha >0$. Namely, $\mu = I^{\alpha/2}U$. We will show that this conjecture is true for planar equal-mass three-body problem under the strong force potential $\sum_{i<j} 1/|q_i-q_j|^2$

Involvement of Fusobacterium Species in Oral Cancer Progression : A Literature Review Including Other Types of Cancer

Chronic inflammation caused by infections has been suggested to be one of the most important cause of cancers. It has recently been shown that there is correlation between intestinal bacteria and cancer development including metastasis. As over 700 bacterial species exist in an oral cavity, it has been concerning that bacterial infection may cause oral cancer. However, the role of bacteria regarding tumorigenesis of oral cancer remains unclear. Several papers have shown that Fusobacterium species deriving the oral cavities, especially, play a crucial role for the development of colorectal and esophageal cancer. F. nucleatum is a well-known oral bacterium involved in formation of typical dental plaque on human teeth and causing periodontal diseases. The greatest characteristic of F. nucleatum is its ability to adhere to various bacteria and host cells. Interestingly, F. nucleatum is frequently detected in oral cancer tissues. Moreover, detection of F. nucleatum is correlated with the clinical stage of oral cancer. Although the detailed mechanism is still unclear, Fusobacterium species have been suggested to be associated with cell adhesion, tumorigenesis, epithelial-to-mesenchymal transition, inflammasomes, cell cycle, etc. in oral cancer. In this review, we introduce the reports focused on the association of Fusobacterium species with cancer development and progression including oral, esophageal, and colon cancers

Saari's homographic conjecture for planar equal-mass three-body problem in Newton gravity

Saari's homographic conjecture in N-body problem under the Newton gravity is the following; configurational measure \mu=\sqrt{I}U, which is the product of square root of the moment of inertia I=(\sum m_k)^{-1}\sum m_i m_j r_{ij}^2 and the potential function U=\sum m_i m_j/r_{ij}, is constant if and only if the motion is homographic. Where m_k represents mass of body k and r_{ij} represents distance between bodies i and j. We prove this conjecture for planar equal-mass three-body problem. In this work, we use three sets of shape variables. In the first step, we use \zeta=3q_3/(2(q_2-q_1)) where q_k \in \mathbb{C} represents position of body k. Using r_1=r_{23}/r_{12} and r_2=r_{31}/r_{12} in intermediate step, we finally use \mu itself and \rho=I^{3/2}/(r_{12}r_{23}r_{31}). The shape variables \mu and \rho make our proof simple

Vision evaluation by functional observational battery, operant behavior test, and light/dark box test in retinal dystrophic RCS rats versus normal rats

BACKGROUND: Vision plays a key role in some behavior tests for rats. Okayama University-type retinal prosthesis (OUReP) is a photoelectric dye-coupled polyethylene film which generates electric potential in response to light and stimulates nearby neurons. This study aims to assess vision in retinal dystrophic (RCS) rats, in comparison with normal rats, by selected behavior tests. We also examined whether the tests could detect vision changes in RCS rats with dye-coupled film implantation. METHODS: Data sets were 5 normal rats, 4 untreated RCS rats, 7 RCS rats with dye-coupled films implanted at the age of 7 weeks after excluding unsuccessful implantation at autopsy. Behavior tests chosen were landing foot splay and visual forelimb-placing response in the menu of functional observational battery, operant-conditioning lever-press response and light/dark box test. RESULTS: Normal visual placing response was significantly less frequent in untreated RCS rats at the age of 9 and 11 weeks, compared with normal rats (P = 0.0027, chi-square test) while normal response was significantly more frequent at the age of 9 weeks in RCS rats with dye-coupled film implantation, compared with untreated RCS rats (P = 0.0221). In operant-conditioning lever-press test, the correct response rate was significantly lower in untreated RCS rats than in normal rats at the age of 9 weeks (P CONCLUSIONS: Behavior tests of functional observational battery, operant-conditioning lever-press response and light/dark box test discriminated vision between normal rats and RCS rats. The visual placing response and operant-conditioning lever-press test might have sensitivity to detect vision recovery in RCS rats with OUReP implantation