Studies on cellular immunity against bile proteins in primary biliary cirrhosis by the leukocyte migration inhibition test (microdroplet method).

Cellular immunity against human bile proteins was investigated by the leukocyte migration inhibition test (LMIT) with 13 primary biliary cirrhosis (PBC) patients, 10 chronic aggressive hepatitis (CAH) patients and 21 healthy adults. Hepatic bile taken from patients operated on for lithiasis of the biliary tract was fractionated into five fractions with Sepharose 6B gel. A subtoxic dose of each fraction was determined in the healthy adults, and used as the antigen for LMIT. Out of the 5 fractions, only the third fraction led to an LMIT positive response in 8 out of 11 (73%) PBC patients and in 1 out of 10 (10%) CAH patients. The difference between PBC and CAH was significant (p less than 0.005). The remaining 3 PBC patients with LMIT negative responses were all under D-penicillamine treatment. Antibody to each fraction was prepared in rabbits. Using the antibodies after absorption with human serum, the localization of the antigens which were present in each fraction was investigated immunohistochemically using human liver sections. The antigen to the anti-first fraction antibody was detected specifically in the epithelial cells of the bile ducts and the ductules, and the antigen to the anti-third fraction antibody was detected specifically on the membrane of the bile canalicules. The third fraction was fractionated into three fractions by Sephadex G-200 gel. Only the first of the 3 fractions showed an LMIT positive response in 3 PBC patients, and its molecular weight was determined to be about 500,000. It is concluded that PBC patients develop cellular immunity against canalicular-antigen-containing fractions but not ductal-antigen-containing ones.</p

体育科目「体育を科学する」の開発と実践

本校は筑波大学と連携してオリンピック・パラリンピック教育の推進を教育重点課題として取り組んできている。平成23年に施行されたスポーツ基本法の施行により、スポーツを基盤とした社会の醸成に寄与できる人材の育成について保健体育科への期待も大きい。本稿ではこうした背景を踏まえ、開発・実践してきた2年次一般科目「体育を科学する」について報告する

平成28年度「卒業研究」実践報告

今年度の卒業研究は、本校のSGHの取り組みと連動する形への移行期として、これまでの卒業研究の取り糾みに修正を加えて実施した。また、国際バカロレア「課題論文」(EE) の指導方法を参考に評価基準を設定することで、生徒がその基準に照らし合わせながら、研究論文を執筆できるようにした。SGHの「国際的に活躍できるグローバルリーダーの育成」の視点と総合学科の「生徒のキャリア教育」の視点を一体化させることで、総合学科ならではのSGH課題研究を行うことができる可能性があるということが明らかとなった

Numerical and Experimental Study on the Development of Electric Sensor as for Measurement of Red Blood Cell Deformability in Microchannels

A microsensor that can continuously measure the deformability of a single red blood cell (RBC) in its microchannels using microelectrodes is described in this paper. The time series of the electric resistance is measured using an AC current vs. voltage method as the RBC passes between counter-electrode-type micro-membrane sensors attached to the bottom wall of the microchannel. The RBC is deformed by the shear flow created in the microchannel; the degree of deformation depends on the elastic modulus of the RBC. The resistance distribution, which is unique to the shape of the RBC, is analyzed to obtain the deformability of each cell. First, a numerical simulation of the electric field around the electrodes and RBC is carried out to evaluate the influences of the RBC height position, channel height, distance between the electrodes, electrode width, and RBC shape on the sensor sensitivity. Then, a microsensor was designed and fabricated on the basis of the numerical results. Resistance measurement was carried out using samples of normal RBCs and rigidified (Ca^[2+]-A23186 treated) RBCs. Visualization measurement of the cells’ behavior was carried out using a high-speed camera, and the results were compared with those obtained above to evaluate the performance of the sensor